{"id":21348,"date":"2019-07-16T12:00:51","date_gmt":"2019-07-16T04:00:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21348"},"modified":"2023-11-24T13:30:05","modified_gmt":"2023-11-24T05:30:05","slug":"%e7%b2%92%e8%85%ba%e9%ab%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-mitochondria-isolation-kit-invent%e8%b2%a8%e8%99%9fmp-007","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21348","title":{"rendered":"\u7c92\u817a\u9ad4\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44 |Mitochondria Isolation Kit Invent\u8ca8\u865fMP-007"},"content":{"rendered":"

\u00a0Minute\u2122<\/a><\/span><\/strong>\u7c92\u817a\u9ad4\u5206\u96e2\u8a66\u5291\u76d2\/ <\/strong>Mitochondria Isolation Kit | <\/span>\u8ca8\u865f<\/strong>MP-007<\/strong><\/a>\u00a0(10-40 X 106<\/sup> cells )<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: Minute\u2122\u7c92\u817a\u9ad4\u5206\u96e2\u8a66\u5291\u76d2(Mitochondria Isolation Kit, MP-007) \uff0c\u53ef\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u548c\u7d44\u7e54\uff1b\u5176\u5546\u54c1\u5177\u6709\u512a\u5316\u7684\u7de9\u885d\u6db2(optimized buffers)\u548c\u86cb\u767d\u8cea\u63d0\u53d6\u6ffe\u82af\u7d44\u6210(protein extraction filter cartridges)\uff0c\u914d\u67092.0 ml\u6536\u96c6\u7ba1\u3002 \u8a72\u8a66\u5291\u76d2\u65e8\u5728\u5f9e\u57f9\u990a\u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u6216\u7d44\u7e54\u4e2d\u5feb\u901f\u5206\u96e2\u5929\u7136\u7c92\u817a\u9ad4\u86cb\u767d(native mitochondria proteins)\u3002 \u86cb\u767d\u8cea\u63d0\u53d6\u6ffe\u7b52 (filter cartridges)\u8a2d\u8a08\uff0c\u4f7f\u86cb\u767d\u8cea\u5206\u96e2\u7a0b\u5e8f\u7c21\u55ae\u4e26\u4e14\u6709\u6548\u63d0\u9ad8\u7522\u7387\u3002 \u8a72\u7a0b\u5e8f\u53ef\u5728\u4e0d\u523030\u5206\u9418\u5167\u5b8c\u6210\u3002 \u8207\u8a31\u591a\u5546\u696d\u7dda\u7c92\u9ad4\u88fd\u5099\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u63d0\u4f9b\u591a\u7a2e\u8d77\u59cb\u7d30\u80de\u548c\u5206\u96e2\u7684\u7c92\u817a\u9ad4\u3002 \u7de9\u6c96\u5291\u4e0d\u542b\u6d17\u6ecc\u5291\u548cEDTA\u3002 \u4e0d\u9700\u8981Dounce\u52fb\u6f3f\u5668\u6216\u7d44\u7e54\u6df7\u5408\u5668\u3002<\/p>\n

\"\"<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Buffer A<\/td>\n15 ml<\/td>\n<\/tr>\n
Buffer B<\/td>\n30 ml<\/td>\n<\/tr>\n
Protein Extraction Filter Cartridges<\/td>\n50 units<\/td>\n<\/tr>\n
Collection Tubes with Caps<\/td>\n50 units<\/td>\n<\/tr>\n
Plastic Rods<\/td>\n2 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute\u2122 Mitochondria Isolation Kit.<\/p>\n

    \n
  1. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues, respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues, respectively.<\/li>\n
  2. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048, a nuclear envelope marker protein. abcam, Cambridge, MA).<\/li>\n
  3. Densitometry measurement of mitochondrial marker signals.<\/li>\n<\/ol>\n

    \u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> | Endosome\u80de\u5167\u9ad4\u5206\u96e2<\/a> | \u51dd\u81a0\u86cb\u767d\u8cea\/\u6838\u9178\u63d0\u53d6<\/a> |<\/span><\/p>\n

    \u5f15\u7528\u6587\u737b<\/a><\/span><\/p>\n

    Gou, R. et al. (2014). Resveratrol suppresses oxidized low-density lipoprotein-induced macrophage apoptosis through inhibition of intracellular reactive oxygen species generation, lox-1 and the p38 MAPK pathway. Cellular Physiology and Biochemistry. 34:603-616.<\/p>\n

    Hu, H., Zhu, W., Qin, J., Chen, M., Gong, L., Li, L., … & Ye, D. (2016). Acetylation of PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology.<\/p>\n

    Gao, G., Wang, Z., Lu, L., Duan, C., Wang, X., & Yang, H. (2017). Morphological analysis of mitochondria for evaluating the toxicity of \u03b1-synuclein in transgenic mice and isolated preparations by atomic force microscopy. Biomedicine & Pharmacotherapy.<\/p>\n

    Zhang, L., Liu, J., Zhou, F., Wang, W., & Chen, N. (2018). PGC-1\u03b1 ameliorates kidney fibrosis in mice with diabetic kidney disease through an antioxidative mechanism. Molecular medicine reports.<\/p>\n

    Wu, Y., Gao, W. N., Xue, Y. N., Zhang, L. C., Zhang, J. J., Lu, S. Y., … & Sun, L. K. (2018). SIRT3 aggravates metformin-induced energy stress and apoptosis in ovarian cancer cells. Experimental Cell Research.<\/p>\n

    Shen, L., Sun, B., Sheng, J., Yu, S., Li, Y., Xu, H., … & Sun, L. (2018). PGC1\u03b1 promotes cisplatin resistance in human ovarian carcinoma cells through upregulation of mitochondrial biogenesis. International Journal of Oncology.<\/p>\n

    Han, W., Cao, F., Gao, X. J., Wang, H. B., Chen, F., Cai, S. J., … & Ding, H. Z. ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. International Journal of Oncology.<\/p>\n

    Ouyang, J., Zeng, Z., Fang, H., Li, F., Zhang, X., & Tan, W. (2019). SIRT3 inactivation promotes acute kidney injury through elevated acetylation of SOD2 and p53. Journal of Surgical Research, 233, 221-230.<\/p>\n

    Zhou, K., Yao, Y. L., He, Z. C., Chen, C., Zhang, X. N., Yang, K. D., … & Niu, Q. (2018). VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells. Cell Death & Disease, 9(10), 988.<\/p>\n

    Xue, Y. N., Yu, B. B., Li, J. L., Guo, R., Zhang, L. C., Sun, L. K., … & Li, Y. (2018). Zinc and p53 Disrupt Mitochondrial Binding of HK2 by Phosphorylating VDAC1. Experimental Cell Research.<\/p>\n

    Chen, X. Y., Ren, H. H., Wang, D., Chen, Y., Qu, C. J., Pan, Z. H., … & Li, D. F. (2019). Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells. Oxidative Medicine and Cellular Longevity, 2019.<\/p>\n

    Ma, J., Chen, L., He, X. X., Wang, Y. J., Yu, H. L., He, Z. X., … & Zhu, X. J. (2019). Functional prediction and characterization of Dip2 gene in mice. Cell biology international.<\/p>\n

    Cai, M., He, P., & Fang, D. L. Hypoxia\u2011induced mitochondrial translocation of DNM1L increases mitochondrial fission and triggers mPTP opening in HCC cells via activation of HK2. Oncology Reports.<\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u00a0Minute\u2122\u7c92\u817a\u9ad4\u5206\u96e2\u8a66\u5291\u76d2\/ Mitochondria Isolation Kit | \u8ca8\u865fMP-007 […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2065],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21348"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21348"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21348\/revisions"}],"predecessor-version":[{"id":47326,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21348\/revisions\/47326"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21348"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21348"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21348"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}