{"id":21288,"date":"2019-07-11T13:26:56","date_gmt":"2019-07-11T05:26:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21288"},"modified":"2023-03-24T10:28:11","modified_gmt":"2023-03-24T02:28:11","slug":"dna-da-tailing-kit-%e8%b2%a8%e8%99%9f-e009","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21288","title":{"rendered":"DNA dA-Tailing Kit | \u8ca8\u865f E009"},"content":{"rendered":"

DNA dA-Tailing Kit | \u8ca8\u865f E009<\/a><\/span><\/strong><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: DNA dA-Tailing Kit\u6709\u6548\u5730\u5c07\u975e\u6a21\u677fdAMP\uff08non-template dAMP , dA\uff09\u6dfb\u52a0\u5230\u5e73\u672b\u7aefDNA\u7247\u6bb5 (blunt-ended DNA fragment) \u76843’\u672b\u7aef\u3002\u8a72\u647b\u5165\u76843′-dA(incorporated 3\u2019-dA) \u9632\u6b62\u591a\u806f\u9ad4(concatemer)\u5f62\u6210\u4e26\u88fd\u5099DNA\u7247\u6bb5(DNA fragment)\u7528\u65bc\u96a8\u5f8c\u9023\u63a5\u5177\u6709\u4e92\u88dc3′-dT\u7a81\u51fa\u7aef(3\u2019-dT overhangs)\u7684\u929c\u63a5\u5b50\u6216\u514b\u9686\u8f09\u9ad4\u3002\u8a72\u8a66\u5291\u76d2\u4e2d\u7684Klenow\u7247\u6bb5\uff083’\u21925’Exo-\uff09\u901a\u904e\u5176DNA\u805a\u5408\u9176\u6d3b\u6027(DNA polymerase activity)\u6dfb\u52a03′-dA\u3002\u9019\u7a2e\u9176\u7f3a\u4e4f5’\u21923’\u6838\u9178\u5916\u5207\u9176\u6d3b\u6027\uff0c\u4e26\u4e14\u5177\u6709\u6709\u6548\u6d88\u9664\u56fa\u6709\u76843’\u21925’\u5916\u5207\u6838\u9178\u9176\u6d3b\u6027\u7684\u7a81\u8b8a\uff0c\u5f9e\u800c\u9632\u6b62\u9019\u7a2e3′-dA\u7684\u964d\u89e3(degradation)\u3002\u8a72\u8a66\u5291\u76d2\u5df2\u7d93\u904e\u512a\u5316\u6e96\u5099\u5177\u517c\u5bb9\u7684overhangs\u3002\u53ef\u70ba\u4e0b\u4e00\u6b65\u88fd\u5099NGS (next generation sequencing) DNA\u6a23\u54c1\u88fd\u5099\uff0c Klenow\u7247\u6bb5\uff083’\u21925’Exo-\uff09\u9176\u4e5f\u53ef\u4f5c\u70ba\u7368\u7acb\u8cfc\u8cb7\uff08\u8ca8\u865fE038\uff09\u3002<\/p>\n\n\n\n\n\n\n\n
Application<\/strong><\/td>\n\n

\u00b7\u00a0 DNA sample preparation<\/p>\n

\u00b7\u00a0 dA-Tailing of 1 – 5 \u03bcg of blunt ended DNA<\/td>\n<\/tr>\n

Image<\/strong><\/td>\n<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore all components at -20\u00b0C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n
Notes<\/strong><\/td>\n\n

One unit is defined as the amount of Klenow Fragment (3\u2019\u21925\u2019 Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37\u00b0C using poly(dA-dT):poly(dA-dT) as a template:primer.<\/p>\n

This product is distributed for laboratory research only.
\nCaution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DNA dA-Tailing Kit | \u8ca8\u865f E009 \u7522\u54c1\u4ecb\u7d39: DNA dA-Tailing Kit\u6709\u6548 […]<\/p>\n","protected":false},"author":3,"featured_media":21291,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2076],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21288"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21288"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21288\/revisions"}],"predecessor-version":[{"id":40449,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21288\/revisions\/40449"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21291"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21288"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21288"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21288"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}