{"id":21172,"date":"2019-07-10T10:07:30","date_gmt":"2019-07-10T02:07:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21172"},"modified":"2022-07-20T11:13:56","modified_gmt":"2022-07-20T03:13:56","slug":"dnafectin2100-%e5%a4%9a%e8%81%9a%e9%99%bd%e9%9b%a2%e5%ad%90%e8%84%82%e8%b3%aa%e9%ab%94liposomes-transfection-reagent-%e8%b2%a8%e8%99%9fg2100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21172","title":{"rendered":"DNAfectin\u21222100 | \u591a\u805a\u967d\u96e2\u5b50&\u8102\u8cea\u9ad4(liposomes) Transfection Reagent \u8ca8\u865fG2100"},"content":{"rendered":"

DNAfectin\u21222100 Transfection Reagent\u00a0 | \u8ca8\u865fG2100 \u00a01ml (1.0mg\/1.0ml )<\/a><\/span><\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"DNAfectin\u21222100 Transfection Reagent\u662f\u4e00\u7a2e\u7368\u7279\u7684\u591a\u805a\u967d\u96e2\u5b50(polycations)\u548c\u8102\u8cea\u9ad4(liposomes)\u914d\u65b9\uff0c\u53ef\u4ee5\u9ad8\u6548\uff0c\u6709\u6548\u5730\u8f49\u67d3\u771f\u6838\u7d30\u80de(eukaryotic cells)\u3002 DNAfectin\u2122\u662f\u8d85\u904e15\u7a2e\u4e0d\u540c\u7d30\u80de\u7cfb\u7684\u6700\u4f73\u8f49\u67d3\u8a66\u5291\uff0c\u5305\u62ec\u96e3\u4ee5\u8f49\u67d3\u7684\u7d30\u80de\uff0c\u61f8\u6d6e\u7d30\u80de (suspension)\u548c\u539f\u4ee3\u7d30\u80de(primary cells)\u3002 \u9019\u7a2e\u8f49\u67d3\u8a66\u5291\u975e\u5e38\u9069\u5408\u4efb\u4f55\u8f49\u67d3\u9700\u6c42\u30025ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA\u3002<\/p>\n

\"\"DNAfectin\u2122 Transfection Reagent is a special and unique formulation of polycations, offering a high transfection efficiency with low cytotoxicity for many cell types, including primary cells. In contrast, DNAfectin\u2122 Plus is a nanoparticle-based transfection reagent. This innovative technology allows DNAfectin\u2122 Plus to achieve superior and reproducible transgene expression levels with no cytotoxic effects. DNAfectin\u2122 Plus also offers a simple protocol that is\u00a0serum-compatible<\/strong>.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CELL LINE<\/th>\nCELL LINE SPECIES\/TISSUE<\/th>\nNUCLEIC ACID<\/th>\nREAGENT<\/th>\nTRANSFECTION TYPE<\/th>\nTRANSFECTION EFFICIENCY(%)<\/th>\n<\/tr>\n
293T<\/a><\/td>\nHuman Embryonic Kidney<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n100%<\/td>\n<\/tr>\n
Jurkat<\/a><\/td>\nHuman T Lymphoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n25%<\/td>\n<\/tr>\n
SHEP<\/a><\/td>\nHuman Neuroblastoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n90%<\/td>\n<\/tr>\n
BOSC23<\/a><\/td>\nHuman Kidney<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n90%<\/td>\n<\/tr>\n
HT1080<\/a><\/td>\nHuman Fibrosarcoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%<\/td>\n<\/tr>\n
C33A<\/a><\/td>\nHuman Cervical Carcinoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n90%<\/td>\n<\/tr>\n
MRC5<\/a><\/td>\nHuman Primary Fibroblast<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%<\/td>\n<\/tr>\n
MDK-Telo<\/a><\/td>\nDog Kidney Cells+Telo<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%<\/td>\n<\/tr>\n
B16<\/a><\/td>\nMurine Melanoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%<\/td>\n<\/tr>\n
CB3<\/a><\/td>\nMouse Erytholeukemia<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n25%<\/td>\n<\/tr>\n
HuVEC<\/a><\/td>\nHuman Ubilical Vein<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n20%<\/td>\n<\/tr>\n
HepG2<\/a><\/td>\nHuman Liver Carcinoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n40%<\/td>\n<\/tr>\n
MMRU<\/a><\/td>\nHuman Melanoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n75%<\/td>\n<\/tr>\n
MMAN<\/a><\/td>\nHuman Melanoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%<\/td>\n<\/tr>\n
MDA-MB-231<\/a><\/td>\nHuman Breast Carcinoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n85%<\/td>\n<\/tr>\n
HeLa<\/a><\/td>\nHuman Cervical Carcinoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%-90%<\/td>\n<\/tr>\n
A2780<\/a><\/td>\nHuman Ovarian Carcinoma<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n70%-75%<\/td>\n<\/tr>\n
L6<\/a><\/td>\nRat Myoblast<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n60%-70%<\/td>\n<\/tr>\n
Rabbit-telo<\/a><\/td>\nTelomerase-Rabbit Cells<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n50%<\/td>\n<\/tr>\n
USO2 (T-USO2)<\/a><\/td>\nHuman Osteosarcoma (tet-inducible)<\/td>\nDNA<\/td>\nDNAfectinTM<\/sup>\u00a02100 Transfection Reagent<\/td>\nTransient<\/td>\n80%-90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e4\u00bf\u0083\u00e9\u008a\u00b7\u00e6\u00b4\u00bb\u00e5\u008b\u0095\"\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DNAfectin\u21222100 Transfection Reagent\u00a0 | \u8ca8\u865fG2100 \u00a01ml (1. […]<\/p>\n","protected":false},"author":3,"featured_media":21173,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[2080],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21172"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21172"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21172\/revisions"}],"predecessor-version":[{"id":28713,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21172\/revisions\/28713"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21173"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21172"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21172"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21172"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}