{"id":21157,"date":"2022-12-07T10:46:35","date_gmt":"2022-12-07T02:46:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21157"},"modified":"2023-07-28T13:49:39","modified_gmt":"2023-07-28T05:49:39","slug":"lb-agar-miller%e9%85%8d%e6%96%b9%e5%9f%b9%e9%a4%8a%e5%9f%ba-%e8%b2%a8%e8%99%9fncm0142a-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21157","title":{"rendered":"LB Agar (Miller\u914d\u65b9)\u57f9\u990a\u57fa | \u8ca8\u865fNCM0142A"},"content":{"rendered":"

6<\/strong>\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/strong><\/a> | \u83cc\u6db2\u6e2c\u91cf<\/strong><\/a>\u00a0<\/strong>(<\/strong>\u5206\u5149\u5149\u5ea6\u8a08<\/strong>)<\/strong>\u00a0<\/strong>\u00a0|\u00a0<\/u>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/strong><\/a>\u00a0|\u00a0<\/strong>Petri dishes <\/strong>\u57f9\u990a\u76bf<\/strong><\/a><\/span><\/p>\n

LB Agar (Miller\u914d\u65b9)\u57f9\u990a\u57fa | \u8ca8\u865fNCM0142A<\/span><\/strong><\/a><\/span><\/p>\n

LB<\/strong>\u57f9\u990a\u57fa<\/strong>\uff08Lysogeny broth<\/strong>\uff0cLB\uff09\u5ee3\u6cdb\u89e3\u91cb\u7232Luria-Bertani\u57f9\u990a\u57fa\uff0c\u4f9d\u64da\u5176\u767c\u660e\u4eba\u8c9d\u723e\u5854\u5c3c\uff08Giuseppe Bertani\uff09 \u7684\u8aaa\u6cd5\uff0c\u9019\u500b\u540d\u5b57\u4f86\u6e90\u65bc\u82f1\u8a9e\u7684 Lysogeny broth\uff0c\u5373\u6eb6\u83cc\u57f9\u990a\u57fa\u3002Luria-Bertani\u57f9\u990a\u57fa\u662f\u5fae\u751f\u7269\u5b78\u5be6\u9a57\u4e2d\u6700\u5e38\u7528\u7684\u71df\u990a\u6027\u57f9\u990a\u57fa\uff0c\u7528\u65bc\u57f9\u990a\u5927\u8178\u687f\u83cc(E. coli<\/em>\u00a0i) \u7b49\u7d30\u83cc\uff0cBroth\u52a0\u5165\u74ca\u8102(Agar)\u88fd\u6210\u7684\u56fa\u614b\u57f9\u990a\u57fa\u3002\u52a0\u5165\u6297\u751f\u7d20\u7684 LB \u57f9\u990a\u57fa(LB Broth) \u53ef\u7528\u65bc\u7be9\u9078\u5927\u8178\u687f\u83cc\u7232\u5bbf\u4e3b\u7684cloning\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> LB Agar\uff08Miller\uff09\u662f\u4e00\u7a2e\u71df\u990a\u8c50\u5bcc\u7684\u57f9\u990a\u57fa\uff0c\u8a2d\u8a08\u7528\u65bc\u57f9\u990a\u91cd\u7d44\u83cc(recombinant strains) \u7684\u7d14\u57f9\u990a\u7269\uff0c\u5b83\u57fa\u65bcMiller\u63cf\u8ff0\u7684\u914d\u65b9\u3002\u5927\u8178\u687f\u83cc\u5728LB\u57f9\u990a\u57fa\u4e2d\u751f\u9577\u81f3\u5c0d\u6578\u665a\u671f (late log phase)\u3002 \u4e00\u4e9bplasmid vectors\u8907\u88fd\u5230high copy number\u4e26\u4e14\u4e0d\u9700\u8981\u9078\u64c7\u6027\u64f4\u589e\u3002 \u4e00\u4e9b\u8f09\u9ad4\u4e0d\u80fd\u5982\u6b64\u81ea\u7531\u5730\u8907\u5236\u4e26\u4e14\u9700\u8981\u88ab\u9078\u64c7\u6027\u5730\u64f4\u589e\u3002 \u53ef\u52a0\u5165\u6c2f\u9ef4\u7d20(Chloramphenicol)\u4ee5\u6291\u5236\u5bbf\u4e3b\u5408\u6210(host synthesis)\u4e26\u9632\u6b62\u7d30\u83cc\u67d3\u8272\u9ad4(bacterial chromosome)\u7684\u8907\u88fd\u3002LB Agar\uff0cMiller\u542b\u670910 g \/ L\u7684\u6c2f\u5316\u9209\uff0c\u8207Lennox\u914d\u65b9\u4e2d\u7684\u542b\u91cf\u4e0d\u540c\u3002 \u9019\u5141\u8a31\u7814\u7a76\u4eba\u54e1\u70ba\u7279\u5b9a\u83cc\u682a\u9078\u64c7\u6700\u4f73\u9e7d\u6fc3\u5ea6\u3002 \u57f9\u990a\u57fa\u53ef\u4ee5\u7121\u83cc\u88dc\u5145\u8461\u8404\u7cd6glucose\u6216\u6297\u751f\u7d20antibiotics\u3002\u8a3b\u660e: Product was previously known as Acumedia Product: 7213 LB Agar (Miller); and LabM Product: LAB168 LB Agar (Miller)\u3002\u6c2f\u5316\u9209\u7684\u91cf\u7d93\u5e38\u96a8\u7d30\u83cc\u7684\u751f\u9577\u9700\u6c42\u4e0d\u540c\u800c\u6709\u6240\u6539\u8b8aLB-Miller (10 g\/L NaCl)\u3001LB-Lennox (5 g\/L NaCl)\u3001LB-Luria (0.5 g\/L NaCl)<\/p>\n

\u80f0\u86cb\u767d\u8156 (Tryptone )————————————- 10.0 g \/ L<\/p>\n

\u9175\u6bcd\u62bd\u63d0\u7269(Yeast Extract ) ———-5.0g \/ L<\/p>\n

Sodium Chloride ————————10.0g \/ L<\/p>\n

Agar _________________15.0 g\/L<\/p>\n

Preparation<\/strong>\u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u5c0740\u514b\u57f9\u990a\u57fa\u6eb6\u65bc1\u5347\u7d14\u6de8\u6c34\u4e2d\u3002\u5982\u6709\u5fc5\u8981\uff0c\u7d93\u5e38\u652a\u62cc\u52a0\u71b1\u4ee5\u5b8c\u5168\u6eb6\u89e3Medium\u3002\u5728121\u2103\u4e0b\u9ad8\u58d3\u6ec5\u83cc15\u5206\u9418\u3002\u7b49\u51b7\u53bb\u5f8c\uff0c\u6700\u7d427.0 \u00b1 0.2 at 25\u00b0C, 25\u2103\u5206\u88dd\u65bcPetri dish\u57f9\u990a\u76bf\u4e2d\u3002<\/p>\n

\u6aa2\u9a57\u65b9\u6cd5\uff1a\u6aa2\u9ad4\u7d93\u7cfb\uf99c\u7a00\u91cb\u5f8c\uff0c\u4ee5\u5e73\u677f\u8a08\uf969\u57f9\u990a\u57fa\u57f9\u990a\u53ca\u8a08\uf969\u4e4b\u65b9\u6cd5\u3002 2.1. \u5de5\u4f5c\u74b0\u5883\uff1a\u5de5\u4f5c\u5e73\u53f0\u9808\u5bec\u655e\u3001\u6f54\u6de8\u3001\u5149\u7dda\uf97c\u597d\uff0c\u64cd\u4f5c\u5e73\u53f0\u5149\ufa01\u70ba 100 \u544e\u71ed\u5149\u4ee5\u4e0a\uff0c\u5bc6\u9589\u5ba4\u5167\u63db\u6c23\uf97c\u597d\uff0c\u5118\u53ef\u80fd\u6c92\u6709\u7070\u5875\u53ca\uf9ca \u52d5\u7a7a\u6c23\u3002\u6bcf 15 \u5206\u9418\uf918\u83cc\uf969\uf967\u5f97\u8d85\u904e 15 CFU\/\u57f9\u990a\u76bf\u3002<\/p>\n\n\n\n\n\n\n\n\n
Product #<\/strong><\/td>\nProduct Description<\/strong><\/td>\n<\/tr>\n
NCM0142A<\/td>\nLB Agar (Miller), 500 g<\/td>\n<\/tr>\n
NCM0142B<\/td>\nLB Agar (Miller), 5 kg<\/td>\n<\/tr>\n
NCM0142C<\/td>\nLB Agar (Miller), 10 kg<\/td>\n<\/tr>\n
NCM0142D<\/td>\nLB Agar (Miller), 25 kg (European only size)<\/td>\n<\/tr>\n
NCM0142E<\/td>\nLB Agar (Miller), 50 kg (US only size)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u66f4\u591a\u8cc7\u6599\u4f86\u81eaFDA\u751f\u83cc\u6578\u4e4b\u6aa2\u9a57 (\u53c3\u8003\u65b9\u6cd5) <\/a><\/span><\/p>\n

\u6587\u737bReferences<\/p>\n

    \n
  1. European Pharmacopoeia 9th Edition (2017) 2. United States Pharmacopeia National Formulary 2017: USP 40 NF 35 3. Japanese Pharmacopeia 17th Edition (2017) 4. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061. 5. Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl\u2019s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.<\/li>\n<\/ol>\n

    \u66f4\u591a\u76f8\u95dc\u5546\u54c1 \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| Agarose |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u6e96\u96fb\u6cf3\u69fd<\/a>| 3M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    6\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1 | \u83cc\u6db2\u6e2c\u91cf\u00a0(\u5206\u5149\u5149\u5ea6\u8a08)\u00a0\u00a0|\u00a0\u6ec5\u83cc\u91dc\u00a0 |\u00a0\u6c34\u6d74\u69fdWaterBath\u00a0|\u00a0\u96fb\u5b50\u5929\u5e73\u00a0| […]<\/p>\n","protected":false},"author":3,"featured_media":7149,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[5086],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21157"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21157"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21157\/revisions"}],"predecessor-version":[{"id":44528,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21157\/revisions\/44528"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7149"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21157"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21157"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21157"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}