{"id":21085,"date":"2019-06-29T16:02:34","date_gmt":"2019-06-29T08:02:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21085"},"modified":"2025-08-05T09:53:42","modified_gmt":"2025-08-05T01:53:42","slug":"malachite-green%e7%a3%b7%e9%85%b8%e6%a0%b9%e6%aa%a2%e6%b8%ac-picolorlock-gold-phosphate-detection-system%e8%b2%a8%e8%99%9f303-0125","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21085","title":{"rendered":"Malachite green\u78f7\u9178\u6839\u6aa2\u6e2c | PiColorLock Gold Phosphate Detection System\u8ca8\u865f303-0125(ABCAM ab270004)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21085\"><strong>\u9ad8\u9748\u654f\u5ea6Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u00a0|\u8ca8\u865f303-0125(ABCAM ab270004)<\/strong><\/a><\/span><\/p>\n<p><span style=\"color: #008000;\"><strong><span style=\"font-size: 12pt;\">PiColorLock Gold Phosphate Detection System<\/span><\/strong><\/span><\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: PiColorLock\u2122\u78f7\u9178\u6839\u6aa2\u6e2c\u8a66\u5291\uff0c\u70ba\u6aa2\u6e2c\u7121\u6a5f\u78f7\u9178\u6839\uff08Pi\uff09\u4e4b\u542b\u91cf\uff1b\u4f7f\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u3002\u53ef\u7528\u65bc\u6e2c\u91cf\u7522\u751fPi\u7684\u4efb\u4f55\u9176\uff0c\u5305\u62ecATPases\u3001GTPases, phosphatases\u3001\u71b1\u4f11\u514b\u86cb\u767d\u53caDNA\u89e3\u65cb\u86cb\u767d\u3002PiColorLock\u2122\u7684\u7368\u7279\u914d\u65b9\u63d0\u9ad8\u4e86\u6e2c\u5b9a\u7dda\u6027\uff0c\u52d5\u614b\u7bc4\u570d\u548c\u984f\u8272\u7a69\u5b9a\u6027\uff1b\u4e5f\u53ef\u4ee5\u7528PiColorLock\u8207\u4e0d\u7a69\u5b9a\u7684\u5e95\u7269\uff08\u4f8b\u5982ATP\uff0cGTP\uff09\u4e00\u8d77\u4f7f\u7528\uff0c\u6240\u8ff0\u5e95\u7269\u8207\u5176\u5b83\u9178\u6027\u67d3\u6599\u57fa\u6aa2\u6e2c\u8a66\u5291\u7522\u751f\u9ad8\u7684\u975e\u9176\u80cc\u666f\u4fe1\u865f\u3002\u6b64\u7522\u54c1\u4e0d\u5177\u653e\u5c04\u6027\u61c9\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\uff0c\u70ba\u9ad8\u9748\u654f\u7684\u78f7\u9178\u6839\u5075\u6e2c\u8a66\u5291\uff0c\u78ba\u4fdd\u7a69\u5b9a\u7684\u7da0\u8272dye-phosphate\u8907\u5408\u7269\u7522\u751f\uff0c\u907f\u514d\u767c\u751f\u53d7\u8cea\u975e\u9175\u7d20\u6c34\u89e3\u5e72\u64fe\u9020\u6210\u7684\u80cc\u666f\u96dc\u8a0a\u3002\u5075\u6e2c\u7bc4\u570d\u5ee3\uff0c\u53ef\u6e1b\u5c11\u6a23\u54c1\u7a00\u91cb\u7684\u5fc5\u8981\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-3193 \" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/09\/picolorlock_web.gif\" alt=\"picolorlock_web\" width=\"298\" height=\"198\" \/><\/p>\n<p><strong>Key benefits:<\/strong><\/p>\n<ul>\n<li>Colorimetric assay \u2013 Simple, scalable and nonradioactive<\/li>\n<li>Compatible with almost any assay buffer \u2013 Flexibility over a wide range of existing assays<\/li>\n<li>Stable reagent formulation \u2013 Long shelf life<\/li>\n<li>Unique accelerator \u2013 Speeds up color development<\/li>\n<li>Unique stabilizer \u2013 Suppresses nonenzymatic backgrounds with acid labile substrates (ATP or GTP)<\/li>\n<li>PiColorLock\u2122-Pi complex is very stable \u2013 No precipitation, results can be measured over several hours; ideal for HTS<\/li>\n<li>Wide linear range \u2013 No inhibition of color development by high concentrations of protein<\/li>\n<li>The unique PiColorLock\u2122 Stabilizer prevents background drift with time.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>Figure 1. Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock\u2122 ensures high stability of the colored dye\u2013phosphate complexes (green color).<\/p>\n<p>&nbsp;<\/p>\n<p>Figure 2. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock\u2122 provides baseline readings.<\/p>\n<p>&nbsp;<\/p>\n<p>Figure 3. PiColorLock\u2122 has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors\u2019 products are linear over a much narrower range of concentrations.<\/p>\n<p>&nbsp;<\/p>\n<table style=\"height: 353px;\" width=\"766\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u7522\u54c1\u578b\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"460\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"112\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">303-0125<br \/>\n303-0500<\/td>\n<td width=\"460\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/phosphate-detection\/picolorlock\/all\/picolorlock-phosphate-detection-system\" target=\"_blank\" rel=\"noopener\">PiColorLock Gold Phosphate Detection System<\/a>\uff08\u9748\u654f\u5ea64\uff0d40uM\uff09<\/td>\n<td style=\"text-align: center;\" width=\"100\">\u6700\u591a1560 assays<br \/>\n\u6700\u591a6250 assays<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"568\"><span style=\"color: #339966;\"><strong>\u95dc\u806f\u5546\u54c1<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">601-9999<\/td>\n<td width=\"460\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/phosphate-detection\/atp-gtp-kits\/all\/10mm-lyophilized-atp\" target=\"_blank\" rel=\"noopener\">10mM Lyophilized ATP<\/a><\/td>\n<td style=\"text-align: center;\" width=\"100\">0.5ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">602-0120<br \/>\n602-0121<br \/>\n602-0122<\/td>\n<td width=\"460\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/phosphate-detection\/atp-gtp-kits\/all\/gtpase-assay-kit\" target=\"_blank\" rel=\"noopener\">High Throughput Colorimetric GTPase Assays<\/a><\/td>\n<td style=\"text-align: center;\" width=\"100\">196 assays<br \/>\n480 assays<br \/>\n5 \u00d7 480 assays<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">501-0015<\/td>\n<td width=\"460\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/phosphate-detection\/affinity-resins\/all\/pibind-resin\" target=\"_blank\" rel=\"noopener\">PiBind resin<\/a>\uff0d\u5feb\u901f\u79fb\u9664\u6a23\u54c1\u4e2d\u6c61\u67d3\u7684\u78f7\u9178\u6839\uff08Pi\uff09<\/td>\n<td style=\"text-align: center;\" width=\"100\">5g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9ad8\u9748\u654f\u5ea6Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u00a0|\u8ca8\u865f303-0125(ABCAM ab270004) P [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":21086,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2101],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21085"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21085"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21085\/revisions"}],"predecessor-version":[{"id":52150,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21085\/revisions\/52150"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21086"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21085"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21085"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21085"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}