{"id":21007,"date":"2019-06-18T16:43:54","date_gmt":"2019-06-18T08:43:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21007"},"modified":"2025-09-26T18:13:37","modified_gmt":"2025-09-26T10:13:37","slug":"streptavidin-hrp-streptomyces-avidinii%e8%b2%a8%e8%99%9f7100-05","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21007","title":{"rendered":"Streptavidin-HRP | Streptomyces avidinii\u8ca8\u865f7100-05 (\u8ca8\u865f\u7570\u52d5\u70ba7105-05)"},"content":{"rendered":"

Streptavidin-HRP | <\/strong>\u8ca8\u865f<\/strong>7100-05 \u00a0<\/strong>\u898f\u683c<\/strong>1ml (HRP\u6a19\u8a8c)\u00a0<\/a><\/span><\/p>\n

\"\" (\u8ca8\u865f\u7570\u52d5\u70ba7105-05)<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

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Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.<\/p>\n

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Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.<\/div>\n
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Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) and then Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.<\/p>\n

Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)<\/p>\n<\/div>\n

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Urine and serum samples were resolved by electrophoresis, transferred to PVDF membrane, and probed with biotinylated anti-human fH followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
\nLane 1 – Purified fH
\nLane 2 – Urine from IgAN patient
\nLane 3 – Serum from IgAN patient
\nLane 4 – Urine from healthy patientImage from Onda K, Ohsawa I, Ohi H, Tamano M, Mano S, Wakabayashi M, et al. Excretion of complement proteins and its activation marker C5b-9 in IgA nephropathy in relation to renal function. BMC Nephrol. 2011;12:64. Figure 4<\/div>\n

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\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Streptavidin-HRP\uff0c\u5177HRP\u6a19\u8a8c\u3002\u91dd\u5c0dBiotin\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baStreptomyces avidinii\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u572850% Glycerol\/50% Phosphate buffered saline, pH 7.4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Source<\/strong><\/td>\nStreptomyces avidinii<\/em><\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nBiotin<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA \u2013 Quality tested\u00a01-7<\/sup>
\nFLISA \u2013 Quality tested
\nFlow Cytometry \u2013 Quality tested\u00a01,2,4,8-15<\/sup>
\nELISpot \u2013 Reported in literature\u00a04,6-8<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a09,10,13,16-19<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a016,18,21<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a011,17,19<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a03,5,20<\/sup>
\nMultiplex \u2013 Reported in literature\u00a022<\/sup>
\nMicroarray \u2013 Reported in literature\u00a023<\/sup>
\nBlocking \u2013 Reported in literature\u00a024<\/sup><\/td>\n<\/tr>\n
Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n

\u6587\u737b\u767c\u8868<\/p>\n

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  1. Hamilton AM, Kearney JF. Effects of IgM allotype suppression on serum IgM levels, B-1 and B-2 cells, and antibody responses in allotype heterozygous F1 mice. Dev Immunol. 1994;4:27-41. (ELISA, FC, IHC-FS)<\/li>\n
  2. Gulbranson-Judge A, Tybulewicz VL, Walters AE, Toellner K, MacLennan IC, Turner M. Defective immunoglobulin class switching in Vav-deficient mice is attributable to compromised T cell help. Eur J Immunol. 1999;29:477-87. (ELISA, FC)<\/li>\n
  3. Utani A, Nomizu M, Matsuura H, Kato K, Kobayashi T, Takeda U, et al. A unique sequence of the laminin \u03b13 G domain binds to heparin and promotes cell adhesion through syndecan-2 and -4. J Biol Chem. 2001;276:28779-88. (ELISA, WB)<\/li>\n
  4. Taylor DK, Ito E, Thorn M, Sundar K, Tedder T, Spatz LA. Loss of tolerance of anti-dsDNA B cells in mice overexpressing CD19. Mol Immunol. 2006;43:1776-90. (ELISA, ELISPOT, FC)<\/li>\n
  5. Doern A, Cao X, Sereno A, Reyes CL, Altshuler A, Huang F, et al. Characterization of inhibitory anti-insulin-like growth factor receptor antibodies with different epitope specificity and ligand-blocking properties: implications for mechanism of action in vivo. J Biol Chem. 2009;284:10254-67. (ELISA, WB)<\/li>\n
  6. Vandenberg CJ, Josefsson EC, Campbell KJ, James C, Lawlor KE, Kile BT, et al. Loss of Bak enhances lymphocytosis but does not ameliorate thrombocytopaenia in BCL-2 transgenic mice. Cell Death Differ. 2014;21:676-84. (ELISA, ELISPOT)<\/li>\n
  7. Dunand CJ, Leon PE, Kaur K, Tan GS, Zheng N, Andrews S, et al. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains. J Clin Invest. 2015;125:1255-68. (ELISA, ELISPOT)<\/li>\n
  8. Ju ZL, Shi GY, Zuo JX, Zhang JW, Sun J. Unexpected development of autoimmunity in BAFF-R-mutant MRL-lpr mice. Immunology. 2006;120:281-9. (ELISPOT, FC)<\/li>\n
  9. Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, et al. Follicular dendritic cell (FDC) precursors in primary lymphoid tissues. J Immunol. 1998;160:1078-84. (FC, IHC-FS)<\/li>\n
  10. Brakebusch C, Fillatreau S, Potocnik AJ, Bungartz G, Wilhelm P, Svensson M, et al. \u03b21 integrin is not essential for hematopoiesis but is necessary for the T cell-dependent IgM antibody response. Immunity. 2002;16:465-77. (FC, IHC-FS)<\/li>\n
  11. Moura IC, Arcos-Fajardo M, Sadaka C, Leroy V, Benhamou M, Novak J, et al. Glycosylation and size of IgA1 are essential for interaction with mesangial transferrin receptor in IgA nephropathy. J Am Soc Nephrol. 2004;15:622-34. (FC, ICC)<\/li>\n
  12. Tsuneto M, Yamazaki H, Yoshino M, Yamada T, Hayashi S. Ascorbic acid promotes osteoclastogenesis from embryonic stem cells. Biochem Biophys Res Commun. 2005;335:1239-46. (FC, Sep)<\/li>\n
  13. Zotos D, Coquet JM, Zhang Y, Light A, D’Costa K, Kallies A, et al. IL-21 regulates germinal center B cell differentiation and proliferation through a B cell-intrinsic mechanism. J Exp Med. 2010;207:365-78. (FC, IHC-FS)<\/li>\n
  14. Thorum SC, Comstock SS, Hester SN, Shunk JM, Monaco MH, Donovan SM. Impact of diet on development of bronchial-associated immunity in the neonatal piglet. Vet Immunol Immunopathol. 2013;151:63-72. (FC)<\/li>\n
  15. Jung S, Schickel J, Kern A, Knapp A, Eftekhari P, Da Silva S, et al. Chronic bacterial infection activates autoreactive B cells and induces isotype switching and autoantigen-driven mutations. Eur J Immunol. 2016;46:131-46. (FC)<\/li>\n
  16. Nosanchuk JD, Valadon P, Feldmesser M, Casadevall A. Melanization of Cryptococcus neoformans in murine infection. Mol Cell Biol. 1999;19:745-50. (IHC-FS, IHC-PS)<\/li>\n
  17. Zannettino AC, Holding CA, Diamond P, Atkins GJ, Kostakis P, Farrugia A, et al. Osteoprotegerin (OPG) is localized to the Weibel-Palade bodies of human vascular endothelial cells and is physically associated with von Willebrand factor. J Cell Physiol. 2005;204:714-23. (IHC-FS, ICC)<\/li>\n
  18. Ji F, Liu Z, Cao J, Li N, Liu Z, Zuo J, et al. B cell response is required for granuloma formation in the early infection of Schistosoma japonicum. PLoS One. 2008;3(3):e1724. (IHC-FS, IHC-PS)<\/li>\n
  19. Piersma B, de Rond S, Werker PM, Boo S, Hinz B, van Beuge MM, et al. YAP1 is a driver of myofibroblast differentiation in normal and diseased fibroblasts. Am J Pathol. 2015;185:3326-37. (IHC-FS, ICC)<\/li>\n
  20. Ren B, Li J, Genschmer K, Hollingshead SK, Briles DE. The absence of PspA or presence of antibody to PspA facilitates the complement-dependent phagocytosis of pneumococci in vitro. Clin Vaccine Immunol. 2012;19:1574-82. (WB)<\/li>\n
  21. El Karoui K, Viau A, Dellis O, Bagattin A, Nguyen C, Baron W, et al. Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2. Nat Commun. 2016;7:10330. (IHC-PS)<\/li>\n
  22. Piccioli D, Tavarini S, Borgogni E, Steri V, Nuti S, Sammicheli C, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. Blood. 2007;109:5371-9. (Multiplex)<\/li>\n
  23. Timmerman P, Barderas R, Desmet J, Altschuh D, Shochat S, Hollestelle MJ, et al. A combinatorial approach for the design of complementarity-determining region-derived peptidomimetics with in vitro anti-tumoral activity. J Biol Chem. 2009;284:34126-34. (Microarray)<\/li>\n
  24. Irie-Maezono R, Tsuyama S. Immunohistochemical analysis of the acid secretion potency in gastric parietal cells. CellBio. 2013;2:179-85. (Block)<\/li>\n<\/ol>\n

     <\/p>\n

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    Streptavidin-HRP | \u8ca8\u865f7100-05 \u00a0\u898f\u683c1ml (HRP\u6a19\u8a8c)\u00a0 (\u8ca8\u865f\u7570\u52d5\u70ba7105 […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[2116],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21007"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21007"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21007\/revisions"}],"predecessor-version":[{"id":52991,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21007\/revisions\/52991"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21007"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21007"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21007"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}