{"id":20862,"date":"2019-06-11T16:45:28","date_gmt":"2019-06-11T08:45:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=20862"},"modified":"2022-07-20T11:41:50","modified_gmt":"2022-07-20T03:41:50","slug":"%ce%b2-%e6%be%b1%e7%b2%89%e6%a8%a3%e8%9b%8b%e7%99%bd%e5%89%8d%e9%ab%94%e6%8a%97%e9%ab%94-beta-amyloid-protein-precursor-app%e8%b2%a8%e8%99%9f5045-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=20862","title":{"rendered":"\u03b2-\u6fb1\u7c89\u6a23\u86cb\u767d\u524d\u9ad4\u6297\u9ad4 | beta-amyloid protein precursor , APP\u8ca8\u865f5045-100"},"content":{"rendered":"

\u03b2-\u6fb1\u7c89\u6a23\u86cb\u767d\u524d\u9ad4\uff08beta-amyloid protein precursor , <\/em><\/strong>APP\uff09\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>5045-100<\/a><\/span><\/p>\n

beta-amyloid protein precursor (APP)<\/em><\/strong><\/p>\n

\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u5927\u8166\u76ae\u8cea\u4e2d\u6fb1\u7c89\u6a23\u86cb\u767d-\u03b2\u80bd\uff08amyloid-beta peptide , Abeta\uff09\u7684\u7a4d\u7d2f\u662f\u963f\u723e\u8328\u6d77\u9ed8\u75c5 (Alzheimer’s disease)\u767c\u75c5\u6a5f\u5236\u4e2d\u7684\u95dc\u9375\u4e8b\u4ef6\u3002\u03b2-\u6fb1\u7c89\u6a23\u86cb\u767d\u524d\u9ad4\uff08beta-amyloid protein precursor , APP\uff09\u88ab\u03b2-\u5206\u6ccc\u9176 (beta-secretase) \u5207\u5272\uff0c\u7522\u751f\u86cb\u767d\u8cea\u7684\u53ef\u6eb6\u6027\u884d\u751f\u7269\u548c\u819c\u9328\u5b9a\u768499-\u6c28\u57fa\u9178\u7fa7\u57fa\u672b\u7aef\u7247\u6bb5\uff08membrane anchored 99-amino acid carboxy-terminal fragment , C99\uff09\u3002 C99\u7247\u6bb5\u5145\u7576\u03b3-\u5206\u6ccc\u9176(gamma-secretase)\u7684\u5e95\u7269\u4ee5\u7522\u751f4kDa\u6fb1\u7c89\u6a23\u86cb\u767d-\u03b2\u80bd\uff08amyloid-beta peptide , Abeta\uff09\uff0c\u5176\u6c89\u7a4d\u5728\u6240\u6709\u963f\u723e\u8328\u6d77\u9ed8(Alzheimer’s disease)\u75c5\u60a3\u8005\u7684\u8166\u4e2d\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\n5045-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 \u00b5g<\/td>\n<\/tr>\n
Antibody Target<\/strong><\/td>\nAPP<\/td>\n<\/tr>\n
Alternate Name<\/strong><\/td>\namyloid-b-peptide, amyloid beta peptide, Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, Cerebral vascular amyloid peptide,<\/td>\n<\/tr>\n
Host<\/strong><\/td>\nRabbit<\/td>\n<\/tr>\n
Antibody Type<\/strong><\/td>\nPolyclonal<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nRabbit IgG1<\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nA peptide corresponding to the amino acids 737 to 751 of APP or 85 to 99 of the C99 fragment generated by b-secretase cleavage.<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nP05067<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n351<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nColorless liquid<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 mg\/ml<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\n100 \u00b5g (1 mg\/ml) in 1X PBS containing 0.02% sodium azide.<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography purified via peptide column.<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman, mouse, rat<\/td>\n<\/tr>\n
Application<\/strong><\/td>\nWestern blot, Immunohistochemistry, ELISA<\/td>\n<\/tr>\n
Positive Control<\/strong><\/td>\nMouse and rat brain tissue lysates<\/td>\n<\/tr>\n
Application & Usage<\/strong><\/td>\nImmunohistochemistry: 2 \u03bcg\/ml; Western Blot: 1 \u2013 2 \u03bcg\/ml; ELISA. However, the optimal conditions should be determined individually.<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nThe antibody solution should be gently mixed before use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\u5373\u7528\u578bIHC\u8a66\u5291\u76d2\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>K405-50<\/a><\/span><\/p>\n

Ready-to-use IHC kit (Biotin free), One-Step HRP Polymer anti-Mouse, Rat and Rabbit IgG (H+L) with DAB<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08Immunohistochemistry , IHC\uff09\/\u514d\u75ab\u7d30\u80de\u5316\u5b78\uff08Immunocytochemistry , ICC\uff09\u662f\u901a\u904e\u4f7f\u7528\u6a19\u8a18\u7684\u6297\u9ad4\u4f5c\u70ba\u7279\u7570\u6027\u8a66\u5291\uff0c\u901a\u904e\u6297\u539f – \u6297\u9ad4\u76f8\u4e92\u4f5c\u7528\u4f86\u5b9a\u4f4d\u7d44\u7e54\u5207\u7247\/\u7d30\u80de\u4e2d\u7684\u6297\u539f\uff0c\u6297\u539f – \u6297\u9ad4\u76f8\u4e92\u4f5c\u7528\u901a\u904e\u8af8\u5982\u87a2\u5149\u67d3\u6599(fluorescent dye) \uff0c\u9176(enzyme,) \uff0c\u653e\u5c04\u6027\u5143\u7d20(radioactive element) \u6216\u81a0\u9ad4\u91d1\u7684\u6a19\u8a18\u7269\u7b49\u8b93\u8868\u73fe\u53ef\u8996\u5316\u3002 \u901a\u5e38\u4f7f\u7528\u5e7e\u7a2eIHC\u6280\u8853\uff0c\u4f8b\u5982\u6a19\u8a18\u7684\u751f\u7269\u7d20\u4e8c\u6297\u93c8\u9ef4\u6297\u751f\u7269\u7d20\u86cb\u767d – \u904e\u6c27\u5316\u7269\u9176 (labeled biotin secondary antibody streptavidin-peroxidase, HRP) \uff0c\u6297HRP-HRP\uff0cABC\u50ac\u5316\u7684\u4fe1\u865f\u653e\u5927\uff0cpolymer system\u805a\u5408\u7269\u7cfb\u7d71\uff08\u4e00\u6b65\u6216\u5169\u6b65\uff09\u7b49\uff0c\u4ee5\u6aa2\u6e2c\u7d44\u7e54\u548c\u7d30\u80de\u4e2d\u7684\u6297\u539f\u3002 BioVision\u7684\u5373\u7528\u578bIHC\u8a66\u5291\u76d2\u63a1\u7528\u805a\u5408\u7269\u6280\u8853( polymer system)\uff0c\u53ef\u63d0\u9ad8\u9748\u654f\u5ea6\u548c\u6aa2\u6e2c\u9748\u654f\u5ea6\u3002 IBSC\u4e00\u6b65\u6cd5\uff0c\u91dd\u5c0d\u6297\u5c0f\u9f20\uff0c\u5927\u9f20\u548c\u5154IgG\uff08H + L\uff09 polymer\uff0c\u4e0d\u540c\u65bc\u514d\u75ab\u751f\u7269\u7d20\/\u6297\u751f\u7269\u7d20\u86cb\u767d\u7684\u7cfb\u7d71 (biotin\/avidin free system)\uff0c\u53ef\u4ee5\u5c0d\u819c (membrane) \uff0c\u7d30\u80de\u8cea (cytoplasmic) \u548c\u6838\u6297\u539f(nuclear antigens) \u76f4\u63a5\u9032\u884c\u67d3\u8272\u3002 \u5b83\u70ba\u7528\u6236\u63d0\u4f9b\u4e86\u5feb\u901f\u4e14\u6613\u65bc\u4f7f\u7528\u7684IHC\u6aa2\u6e2c\u7cfb\u7d71\u3002<\/p>\n

\u00a0\"\"<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK405-50<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 tests<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMouse, rat, and rabbit<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nA biotin\/avidin free system that stains membrane, cytoplasmic and nuclear antigens. Immunohistochemistry (IHC) and immunocytochemistry (ICC).<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 3 hours
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive and stable
\n\u2022 The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Ready-to-use, peroxidase block, hydrogen peroxide
\n\u2022 Ready-to-use protein blocking solutions
\n\u2022 Primary antibody dilution buffer
\n\u2022 IBSC-one-step HRP-anti-mouse, rat, and rabbit polymer
\n\u2022 Reagent BS (ready to use buffer & substrate)
\n\u2022 Reagent C (conc. DAB chromogen)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n2-8\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u03b2-\u6fb1\u7c89\u6a23\u86cb\u767d\u524d\u9ad4\uff08beta-amyloid protein precursor , APP\uff09\u6297\u9ad4 | \u8ca8\u865f5 […]<\/p>\n","protected":false},"author":3,"featured_media":5871,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2129,2128],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20862"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=20862"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20862\/revisions"}],"predecessor-version":[{"id":20865,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20862\/revisions\/20865"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5871"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20862"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=20862"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=20862"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}