{"id":20832,"date":"2019-06-10T11:19:41","date_gmt":"2019-06-10T03:19:41","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=20832"},"modified":"2022-11-03T17:08:34","modified_gmt":"2022-11-03T09:08:34","slug":"protein-a-hrp-%e8%9b%8b%e7%99%bdaprotein-a-hrp%e8%b2%a8%e8%99%9f7300-05","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=20832","title":{"rendered":"Protein A-HRP | \u86cb\u767dA(Protein A)-HRP\u8ca8\u865f7300-05"},"content":{"rendered":"

Protein A-HRP\uff0c\u5177HRP\u6a19\u8a8c | <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>7300-05<\/a><\/span> \u00a0<\/strong>\u898f\u683c<\/strong>1.0 ml<\/p>\n

\u86cb\u767dA(Protein A)-HRP<\/strong><\/p>\n

\u86cb\u767dA(Protein A)\u662f\u6700\u521d\u5728\u7d30\u83cc\u91d1\u9ec3\u8272\u8461\u8404\u7403\u83cc(bacterium Staphylococcus aureus)\u7684\u7d30\u80de\u58c1\u4e2d\u767c\u73fe\u768442kD\u8868\u9762\u86cb\u767d (surface protein)\u3002 \u7531\u65bc\u5176\u7d50\u5408\u514d\u75ab\u7403\u86cb\u767d(immunoglobulins)\u7684\u80fd\u529b\uff0c\u5b83\u88ab\u5ee3\u6cdb\u7528\u65bc\u751f\u7269\u5316\u5b78\u7814\u7a76(biochemical research)\u548c\u5de5\u696d(industry)\u3002 \u5b83\u7531\u4e94\u500b\u540c\u6e90\u7684Ig\u7d50\u5408\u7d50\u69cb\u57df(homologous Ig-binding domains)\u7d44\u6210\uff0c\u6298\u758a\u6210\u4e09\u87ba\u65cb\u675f(three-helix bundle)\u3002 \u6bcf\u500b\u7d50\u69cb\u57df\u80fd\u5920\u7d50\u5408\u4f86\u81ea\u8a31\u591a\u54fa\u4e73\u52d5\u7269\u7269\u7a2e\u7684\u86cb\u767d\u8cea\uff0c\u6700\u503c\u5f97\u6ce8\u610f\u7684\u662fIgG(immunoglobulins)\u3002 \u5b83\u7d50\u5408\u5927\u591a\u6578\u514d\u75ab\u7403\u86cb\u767d\u7684Fc\u5340\u5167\u7684\u91cd\u93c8(heavy chain)\u3002\u5c07\u86cb\u767dA\u6a19\u8a8cHRP\uff0c\u53ef\u61c9\u7528\u65bc\u6aa2\u6e2c\u4e00\u6297\uff0c\u4f8b\u5982ELISA\uff0cELISPOT\uff0cIHC\u6216Western\u5370\u8de1 (Western blotting) \u3002 IP\u96a8\u5f8c\u9032\u884cWestern blotting\u61c9\u7528\uff0cHRP\u86cb\u767dA\u5c07\u907f\u514dIgG\u91cd\u93c8\/\u8f15\u93c8(heavy\/light chain)\u554f\u984c\uff0c\u56e0\u70ba\u5b83\u50c5\u8b58\u5225\u5176\u5929\u7136\u5f62\u5f0f\u7684IgG\uff0c\u4e26\u4e14\u7528\u65bcIP\u9776\u6a19\u7684\u6297\u9ad4\u901a\u5e38\u8207\u514d\u75ab\u6c89\u6fb1\u7269\u4e00\u8d77\u716e\u6cb8\u4e26\u8b8a\u6027\u3002<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

Protein A-HRP\uff0c\u5177HRP\u6a19\u8a8c\u3002\u91dd\u5c0dBinds the Fc portion of human IgG1, IgG2, and IgG4 as well as mouse IgG2aand IgG2b; also binds rabbit, porcine, guinea pig, and canine IgG\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baStaphylococcus aureus\u3002\u7de9\u885d\u6db2\u914d\u65b9\u572850% Glycerol\/50% Phosphate buffered saline, pH 7.4\u3002\u7d50\u69cbMonomeric protein lacking cysteine residues; Mr 42 kDa<\/p>\n\n\n\n\n\n\n\n\n\n
Source<\/strong><\/td>\nStaphylococcus aureus<\/em><\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nBinds the Fc portion of human IgG1<\/sub>, IgG2<\/sub>, and IgG4<\/sub>\u00a0as well as mouse IgG2a<\/sub>and IgG2b<\/sub>; also binds rabbit, porcine, guinea pig, and canine IgG<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA \u2013 Quality tested\u00a01-7<\/sup>
\nPurification
\nWB\u00a08<\/sup>
\nIP<\/td>\n<\/tr>\n
Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u5f15\u7528\u6587\u737b\"<\/p>\n

    \n
  1. Sondgeroth K, Blitvich B, Blair C, Terwee J, Junge R, Sauther M, et al. Assessing flavivirus, lentivirus, and herpesvirus exposure in free-ranging ring-tailed lemurs in southwestern Madagascar. J Wildl Dis. 2007;43:40-7. (ELISA)<\/li>\n
  2. Wycoff KL, Belle A, Deppe D, Schaefer L, Maclean JM, Haase S, et al. Recombinant anthrax toxin receptor-Fc fusion proteins produced in plants protect rabbits against inhalational anthrax. Antimicrob Agents Chemother. 2011;55:132-9. (ELISA)<\/li>\n
  3. Duthie MS, Truman RW, Goto W, O’Donnell J, Hay MN, Spencer JS, et al. Insight toward early diagnosis of leprosy through analysis of the developing antibody responses of Mycobacterium leprae-infected armadillos. Clin Vaccine Immunol. 2011;18:254-9. (ELISA)<\/li>\n
  4. Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein. Int J Mol Sci. 2011;12:4975-90. (ELISA)<\/li>\n
  5. Karuppanan K, Duhra-Gill S, Kailemia MJ, Phu ML, Lebrilla CB, Dandekar AM, et al. Expression, purification, and biophysical characterization of a secreted anthrax decoy fusion protein in Nicotiana benthamiana. Int J Mol Sci. 2017;18:89. (ELISA)<\/li>\n
  6. Xiong Y, Li Q, Kailemia MJ, Lebrilla CB, Nandi S, McDonald KA. Glycoform modification of secreted recombinant glycoproteins through kifunensine addition during transient vacuum agroinfiltration. Int J Mol Sci. 2018;19:890. (ELISA)<\/li>\n
  7. Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA. Transient recombinant protein production in glycoengineered Nicotiana benthamiana cell suspension culture. Int J Mol Sci. 2018;19:1205. (ELISA)<\/li>\n
  8. Naskar P, Puri N. Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis. Biol Open. 2017;6:1257-69. (WB)<\/li>\n<\/ol>\n

    SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0<\/span>\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Protein A-HRP\uff0c\u5177HRP\u6a19\u8a8c | \u8ca8\u865f7300-05 \u00a0\u898f\u683c1.0 ml \u86cb\u767dA(Protein […]<\/p>\n","protected":false},"author":3,"featured_media":6751,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2134],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20832"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=20832"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20832\/revisions"}],"predecessor-version":[{"id":35789,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20832\/revisions\/35789"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6751"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20832"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=20832"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=20832"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}