{"id":19942,"date":"2019-05-21T17:19:31","date_gmt":"2019-05-21T09:19:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19942"},"modified":"2023-05-23T12:16:24","modified_gmt":"2023-05-23T04:16:24","slug":"%e8%84%82%e8%a7%a3%ef%bc%883t3-l1%ef%bc%89%e6%af%94%e8%89%b2%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk577-100-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19942","title":{"rendered":"\u8102\u89e3\uff083T3-L1\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK577-100 (ABCAM ab185433)"},"content":{"rendered":"

\u8102\u89e3\uff083T3-L1\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK577-100(ABCAM ab185433)<\/span><\/strong><\/span><\/a><\/span><\/p>\n

Lipolysis (3T3-L1) Colorimetric Assay Kit<\/em>\u00a0<\/em><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8102\u80aa\u5206\u89e3 (Lipolysis) \u662f\u7d30\u80de\u5167\u7518\u6cb9\u4e09\u916f(triglycerides)\u6c34\u89e3\u6210\u7518\u6cb9(glycerol)\u548c\u6e38\u96e2\u8102\u80aa\u9178(free fatty acids)\u3002\u7136\u5f8c\u5c07\u7518\u6cb9\uff08glycerol\uff09\u548c\u6e38\u96e2\u8102\u80aa\u9178(free fatty acids)\u91cb\u653e\u5230\u8840\u6d41(bloodstream)\u6216\u57f9\u990a\u57fa(culture media)\u4e2d\u3002\u8102\u89e3\u4f5c\u7528(Lipolysis) \u57fa\u672c\u4e0a\u5728\u6240\u6709\u7d30\u80de\u4e2d\u767c\u751f\uff0c\u4f46\u5728\u767d\u8272(white adipose tissue)\u548c\u68d5\u8272\u8102\u80aa\u7d44\u7e54(brown adipose tissue)\u4e2d\u6700\u8c50\u5bcc\u3002\u8102\u89e3\u4f5c\u7528(lipolysis)\u7684\u7f3a\u4e4f\u5c0e\u81f4\u7d30\u80de\u5167\u8102\u8cea\u7a4d\u7d2f\u589e\u52a0\uff0c\u5c0e\u81f4\u7570\u5e38\u7684\u7d30\u80de\u751f\u7406\u5b78\uff0c\u9ad8\u8102\u8840\u75c7(hyperlipidemia)\u548c\u80f0\u5cf6\u7d20\u62b5\u6297(insulin resistance)\u3002\u5152\u8336\u915a\u80fa(catecholamines)\u548c\u67d0\u4e9b\u6fc0\u7d20(certain hormones)\u53ef\u8a98\u5c0e\u8102\u80aa\u5206\u89e3\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ec\u5408\u6210\u7684\u5152\u8336\u915a\u80fa(catecholamines)\uff0c\u7570\u4e19\u814e\u4e0a\u817a\u7d20(Isoproterenol)\uff0c\u53ef\u6fc0\u6d3b\u03b2-\u814e\u4e0a\u817a\u7d20\u80fd\u53d7\u9ad4(\u03b2-adrenergic receptors) \u3002\u9019\u5c0e\u81f4\u817a\u82f7\u9178\u74b0\u5316\u9176(adenylate cyclase) \u7684\u6d3b\u5316\uff0c\u5176\u50ac\u5316ATP\u8f49\u5316\u70bacAMP\u3002\u7136\u5f8ccAMP\u4f5c\u70ba\u7b2c\u4e8c\u4fe1\u4f7f\u6fc0\u6d3b\u6fc0\u7d20\u654f\u611f\u6027\u8102\u80aa\u9176\uff0c\u5176\u6c34\u89e3\u7518\u6cb9\u4e09\u916f(triglycerides)\u3002\u80f0\u5cf6\u7d20(insulin)\u53ef\u4ee5\u6291\u5236\u8a72\u9014\u5f91\u3002 BioVision\u76843T3-L1\u8102\u80aa\u5206\u89e3\u8a66\u5291\u76d2\u7c21\u55ae\u6613\u7528\u3002\u8a72\u6e2c\u5b9a\u6cd5\u6e2c\u91cf\u5728\u4f7f\u7528\u6bd4\u8272\u6cd5\u8a98\u5c0e\u8102\u89e3\u5f8c\u5f9e3T3-L1\u7d30\u80de\u91cb\u653e\u7684\u7518\u6cb9(glycerol)\u3002\u984f\u8272\u5f37\u5ea6\u8207\u7518\u6cb9\u7684\u91cf\u6210\u6b63\u6bd4\u3002\u8a72\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c\u5c11\u65bc200pmol\u7684\u7518\u6cb9(glycerol\u3002<\/p>\n

<\/p>\n

3T3-L1 \u7d30\u80de\u57f9\u990a\u57fa\u4e2d\u7518\u6cb9\u6c34\u5e73\u7684\u6e2c\u91cf\u3002<\/strong><\/span><\/div>\n
\n

50 \u03bcl 3T3-L1 \u7d30\u80de\u7528\u8f09\u9ad4\u5c0d\u7167 (H\u00a0<\/span>2<\/span><\/sub>\u00a0O) \u6216 100 nM \u7570\u4e19\u814e\u4e0a\u817a\u7d20\u8655\u7406 0-3 \u5c0f\u6642\u3002<\/span><\/p>\n<\/div>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK577-100(ABCAM ab185433)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 570 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of lipolysis in 3T3-L1 cells or adipocytes
\nScreening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient
\n\u2022 The assay can detect less than 200 pmol of glycerol.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Lipolysis Assay Buffer
\n\u2022 Lipolysis Wash Buffer
\n\u2022 Glycerol Assay Buffer
\n\u2022 Glycerol Probe (in DMSO, Anhydrous)
\n\u2022 Glycerol Enzyme Mix (Lyophilized)
\n\u2022 Glycerol Standard (100 mM)
\n\u2022 Isoproterenol (10 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8102\u89e3\uff083T3-L1\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK577-100(ABCAM ab185433) Lipolysi […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[2175],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19942"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19942"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19942\/revisions"}],"predecessor-version":[{"id":42610,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19942\/revisions\/42610"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19942"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19942"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19942"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}