{"id":19895,"date":"2019-05-20T16:59:39","date_gmt":"2019-05-20T08:59:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19895"},"modified":"2022-07-20T13:47:36","modified_gmt":"2022-07-20T05:47:36","slug":"hsa-mir-3614-mirna%e6%85%a2%e7%97%85%e6%af%92%e8%a1%a8%e7%8f%be%e8%bc%89%e9%ab%94-lentimira-gfp-hsa-mir-3614-vector-%e8%b2%a8%e8%99%9fmh10493","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19895","title":{"rendered":"(hsa-mir-3614) miRNA\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | LentimiRa-GFP-hsa-mir-3614 Vector \u8ca8\u865fmh10493"},"content":{"rendered":"

mir-34a\u00a0<\/a>\u00a0|<\/span>\u00a0mir-7-1<\/strong>\u00a0<\/span><\/a>\u00a0|\u00a0mir-3614\u00a0<\/a>\u00a0|\u00a0mir-21\u00a0<\/a>\u00a0|\u00a0mir-185\u00a0<\/a>\u00a0<\/strong><\/span><\/strong><\/p>\n

hsa-mir-3614\u00a0miRNA\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | LentimiRa-GFP-hsa-mir-3614 Vector \u8ca8\u865fmh10493<\/a>\u00a0<\/span><\/span><\/p>\n

hsa-mir-3614\u00a0 miRNA (miRNA ID MI0016004) \u8f09\u9ad4\u8cc7\u6599pLenti-III-mir-GFP<\/span><\/p>\n

\u91cd\u7d44\u6162\u75c5\u6bd2\u8868\u9054\u8f09\u9ad4 (Recombinant lentiviral expression vectors) \u662f\u7528\u65bcmiRNA\u905e\u9001 (miRNA delivery) \u7684\u6700\u5ee3\u6cdb\u4f7f\u7528\u7684\u905e\u9001\u8f09\u9ad4\uff0c\u7531\u65bc\u5176\u9ad8\u6548\u8f49\u5c0e(high efficiency transduction) \u548c\u7a69\u5b9a\u6574\u5408 (stable integration)\u3002abm\u63d0\u4f9b\u4e86\u6700\u5168\u9762\u7684miRNA\u6162\u75c5\u6bd2\u8868\u9054\u8f09\u9ad4\uff08lentimira\uff09\u9078\u64c7\u3002 \u6e2c\u8a66\u4e86\u6bcf\u7a2e\u8f09\u9ad4\u7684\u529f\u80fd\u6027\uff0c\u5728\u8cea\u9ad4\u8f09\u9ad4\u7cfb\u5217\u7522\u54c1\uff0c\u6216\u662f\u548cready-to-use\u5373\u7528\u578b\u75c5\u6bd2\u9846\u7c92\u5f62\u5f0f\uff0cCMV\u555f\u52d5\u5b50\u4e0b\u542b\u6709GFP\u5831\u544a\u57fa\u56e0(constitutive CMV promoter)\u3002\u5c0d\u65bc\u6bcf\u7a2emiRNA\uff0c\u5929\u7136pri-miRNA\uff08native pri-miRNA ~400-500bp\uff09\u7684\u8a2d\u8a08\uff0c\u6b64\u8a2d\u8a08\u63d0\u4f9b\u55ae\u4e00\u5e38\u898bmiRNA\u9aa8\u67b6\u7684\u57fa\u56e0\u6291\u5236\uff0c\u5177\u66f4\u66f4\u9ad8\u6c34\u5e73\u7684\u9776\u57fa\u56e0\u6291\u5236 (target gene suppression)\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:\u00a0<\/strong> hsa-mir-3614\u00a0<\/span>\u00a0miRNA (miRNA ID MI0016004)<\/u>\u00a0\u67b6\u69cb\u65bcpLenti-III-mir-GFP<\/a><\/span>\u8868\u73fe\u8f09\u9ad4\u4e0a\uff0c\u5177\u6709GFP\u5831\u5c0e\u57fa\u56e0\uff0c\u69cb\u6210LentimiRa-GFP-hsa-mir-3614 Vector\u3002<\/strong>miRNA\u6162\u75c5\u6bd2\u8f09\u9ad4\uff0c\u53ef\u7d50\u5408packaging mix (\u6539\u826f\u5f0f\u00a0\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u00a0<\/a>LV003<\/span>)\u00a0<\/strong>\u4ee5\u5728\u591a\u7a2e\u5bbf\u4e3b\u7d30\u80de\u6216\u52d5\u7269\u6a21\u578b\u4e2d\u904e\u8868\u9054\u76ee\u6a19miRNA\u3002 \u8a72\u69cb\u5efa\u9ad4\u542b\u6709\u7528\u65bc\u76e3\u6e2c\u8f49\u67d3\u6548\u7387\u7684GFP\u5831\u544a\u57fa\u56e0(GFP reporter )\uff0c\u53ef\u4ee5Monitor\u8f49\u67d3\u6548\u7387\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u8f49\u67d3 (non-viral plasmid transfection) \u7acb\u5373\u8868\u9054\u76ee\u6a19miRNA\uff0c\u6216\u7d93\u7531\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\uff0c\u5be6\u73fe\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u7684\u8868\u9054\u76ee\u6a19miRNA\u3002Control vector\u00a0pLenti-III-mir-GFP Control Vector<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Application<\/strong><\/td>\n\n

Direct non-viral plasmid transfection for immediate expression<\/p>\n

Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n

miRNA ID<\/strong><\/td>\nMI0016004<\/td>\n<\/tr>\n
miRNA Name<\/strong><\/td>\nhsa-mir-3614<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nThis miRNA lentiviral vector is part of\u00a0abm<\/strong>\u2019s Lentivirus Expression System and can be packaged into virus using an appropriate packaging mix to over-express your miRNA of interest in a wide range of host cells or animal models. This construct contains a GFP reporter for monitoring transfection efficiency.<\/td>\n<\/tr>\n
Accession No.<\/strong><\/td>\nMI0016004<\/a><\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Insert Type<\/strong><\/td>\nPre-miRNA<\/td>\n<\/tr>\n
Reporter<\/strong><\/td>\nGFP<\/td>\n<\/tr>\n
Vector Name<\/strong><\/td>\npLenti-III-mir-GFP<\/a><\/td>\n<\/tr>\n
Vector Map<\/strong><\/td>\n\u00a0\"\"<\/td>\n<\/tr>\n
Viral Titer<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Control Vector<\/strong><\/td>\npLenti-III-mir-GFP Control Vector<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\n\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0\u6539\u826f\u5f0f\u00a0\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u00a0<\/a>LV003 |\u00a0\u00a0Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a>\u00a0|\u00a0Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316)\u00a0<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

mir-34a\u00a0\u00a0|\u00a0mir-7-1\u00a0\u00a0|\u00a0mir-3614\u00a0\u00a0|\u00a0mir-21\u00a0\u00a0|\u00a0mir-185\u00a0\u00a0 h […]<\/p>\n","protected":false},"author":3,"featured_media":19853,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[116],"tags":[2182],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19895"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19895"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19895\/revisions"}],"predecessor-version":[{"id":19939,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19895\/revisions\/19939"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19853"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19895"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19895"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19895"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}