{"id":19776,"date":"2019-05-09T15:11:23","date_gmt":"2019-05-09T07:11:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19776"},"modified":"2022-07-20T13:50:58","modified_gmt":"2022-07-20T05:50:58","slug":"mouse-anti-chicken-cd8%ce%b1-pe%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f8220-09","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19776","title":{"rendered":"Mouse Anti-Chicken CD8\u03b1-PE\u6297\u9ad4 | \u8ca8\u865f8220-09"},"content":{"rendered":"

Mouse Anti-Chicken CD8\u03b1-PE\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>8220-09<\/a><\/span>\u00a0 \u00a0<\/strong>\u898f\u683c<\/strong>1.0 ml \u00a0(1.0 mg\/mL)<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0\u5728\u96de\u4e2d\uff0cCD8\u5206\u5b50\u4ee5\u5169\u7a2e\u5f62\u5f0f\u5b58\u5728 – \uff08i\uff09\u5169\u689d\u03b1\u93c8\u7684\u540c\u578b\u4e8c\u805a\u9ad4(homodimer) \u548c\uff08ii\uff09\u03b1\u934a\u548c\u03b2\u93c8\u7684\u7570\u4e8c\u805a\u9ad4 (heterodimer) \u3002 \u96d6\u7136\u6210\u5e74\u96de\u7684\u80f8\u817a(thymus) \uff0c\u813e\u81df(spleen) \u548c\u8840\u6db2(blood)\u4e2d\u7684\u7d55\u5927\u591a\u6578CD8 +\u7d30\u80de\u5747\u8868\u9054CD8\u03b1-\u548cCD8\u03b2-\u93c8\uff0c\u4f46\u80da\u80ce\u548c\u5e7c\u96db\u813e\u81df\u4e2d\u76f8\u5c0d\u5927\u6bd4\u4f8b\u7684CD8 +TCR\u03b3\u03b4\u7d30\u80de\u50c5\u8868\u9054\u03b1-\u93c8\u3002 CD8 \u5728\u8178\u4e0a\u76ae\u6dcb\u5df4\u7d30\u80de\u4e2d\uff0c\u5c0f\u9f20\u4e2d\u5b58\u5728\u7684\u4e3b\u8981CD8 + T\u7d30\u80de\u7fa4\u5177\u4fdd\u5b88\uff0c\u4f46\u662f\u5728\u56d3\u9f52\u52d5\u7269\u4e2d\u6c92\u6709\u767c\u73fe\u96de\u4e2d\u7684TCR\u03b3\u03b4CD8\u03b1\u03b2\u7d30\u80de\u7fa4\u3002 \u96deCD8\u5728\u5927\u7d0480\uff05\u7684\u80f8\u817a\u7d30\u80de (thymocytes) \uff0c15\uff05\u7684\u8840\u6db2\u55ae\u6838\u7d30\u80de(blood mononuclear cells) \u548c50\uff05\u7684\u813e\u7d30\u80de\uff08spleen cells\uff09\u3000\u4e2d\u8868\u9054\uff0c\u4f46\u5728\u6cd5\u6c0f\u56ca\u548c\u9aa8\u9ad3\u4e2d\u7684\u8868\u9054\u4e0d\u52301\uff05\u3002 \u55ae\u514b\u9686\u6297\u9ad4CT-8\u8b58\u5225CD8\u03b1\u93c8\uff0c\u4e26\u4e14\u5df2\u986f\u793a\u51fa\u8207\u706b\u96de\u4e2d\u7684\u591a\u614b\u6027\u6c7a\u5b9a\u7c07\u53cd\u61c9\uff08polymorphic determinant\uff09\u3002<\/p>\n

\"\"<\/p>\n

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8\u03b1-PE (SB Cat. No. 8220-09) and Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nCT-8<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-PE<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nChicken thymocytes and Ig-negative blood leukocytes<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken\/Turkey\/Guinea Fowl\/Pigeon CD8\u03b1<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nIn the chicken, the CD8 molecule is present in two forms – (i) a homodimer of two \u03b1 chains and (ii) a heterodimer of an \u03b1 chain and a \u03b2 chain. While the vast majority of CD8+<\/sup>\u00a0cells in the thymus, spleen, and blood of adult chickens express both CD8\u03b1- and CD8\u03b2-chains, a relatively large proportion of the CD8+<\/sup>\u00a0TCR\u03b3\u03b4 cells in the spleens of embryos and young chicks express only the \u03b1-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+<\/sup>\u00a0T cell populations present in mice are conserved but there is a population of TCR\u03b3\u03b4 CD8\u03b1\u03b2 cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8\u03b1 chain and has been shown to react to a polymorphic determinant in turkey.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nPE (R-phycoerythrin)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide and a stabilizer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.1 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light; Do not freeze
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,9-16,18<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a02-5<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a06-8<\/sup>
\nImmunocytochemistry – Reported in literature\u00a017<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01,17<\/sup>
\nBlocking \u2013 Reported in literature\u00a01<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Mouse Anti-Chicken CD8\u03b1-PE\u6297\u9ad4 | \u8ca8\u865f8220-09\u00a0 \u00a0\u898f\u683c1.0 ml \u00a0(1 […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2208],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19776"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19776"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19776\/revisions"}],"predecessor-version":[{"id":19777,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19776\/revisions\/19777"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19776"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19776"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}