{"id":19631,"date":"2019-04-29T16:43:43","date_gmt":"2019-04-29T08:43:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19631"},"modified":"2022-07-20T13:59:56","modified_gmt":"2022-07-20T05:59:56","slug":"mouse-anti-chicken-cd8%ce%b2-pe%e6%8a%97%e9%ab%94-southernbiotech-sba%e8%b2%a8%e8%99%9f8280-09","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19631","title":{"rendered":"Mouse Anti-Chicken CD8\u03b2-PE\u6297\u9ad4 | Southernbiotech SBA\u8ca8\u865f8280-09"},"content":{"rendered":"

Mouse Anti-Chicken CD8\u03b2-PE\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>8280-09<\/a><\/span>\u00a0 \u898f\u683c<\/strong>1.0 mL \u00a0(0.1 mg\/mL)<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u5728\u96de\u4e2d\uff0cCD8\u5206\u5b50\u4ee5\u5169\u7a2e\u5f62\u5f0f\u5b58\u5728 – \uff08i\uff09\u5169\u689d\u03b1\u93c8\u7684\u540c\u578b\u4e8c\u805a\u9ad4 (homodimer) \u548c\uff08ii\uff09\u03b1\u934a\u548c\u03b2\u93c8\u7684\u7570\u4e8c\u805a\u9ad4 (heterodimer)\u3002 \u96d6\u7136\u6210\u5e74\u96de\u7684\u80f8\u817a(thymus)\u3001\u813e\u81df(spleen)\u3001\u548c\u8840\u6db2(blood)\u4e2d\u7684\u7d55\u5927\u591a\u6578CD8 +\u7d30\u80de\u5747\u8868\u9054CD8\u03b1-\u548cCD8\u03b2-\u93c8\uff0c\u4f46\u80da\u80ce(embryos)\u548c\u5e7c\u96db\u813e\u81df\u4e2d\u76f8\u5c0d\u5927\u6bd4\u4f8b\u7684CD8 + TCR-\u03b3\u03b4\u7d30\u80de\u50c5\u8868\u9054\u03b1\u00a0 –\u00a0 CD8\u7684\u93c8\u3002 \u5728\u8178\u4e0a\u76ae\u6dcb\u5df4\u7d30\u80de (intestinal epithelial lymphocytes)\u4e2d\uff0c\u5c0f\u9f20\u4e2d\u5b58\u5728\u7684\u4e3b\u8981CD8 + T\u7d30\u80de\u7fa4\u662f\u4fdd\u5b88 (conserved) \u7684\uff0c\u4f46\u5728\u96de\u4e2d\u5b58\u5728TCR-\u03b3\u03b4CD8\u03b1\u03b2\u7d30\u80de\u7fa4\uff0c\u5176\u5728\u56d3\u9f52\u52d5\u7269 (rodents) \u4e2d\u672a\u767c\u73fe\u3002 \u96deCD8\u5728\u5927\u7d0480\uff05\u7684\u80f8\u817a\u7d30\u80de (thymocytes)\uff0c45\uff05\u7684\u8840\u6db2\u55ae\u6838\u7d30\u80de (blood mononuclear cells) \u548c50\uff05\u7684\u813e\u7d30\u80de (spleen cells) \u4e2d\u8868\u9054\uff0c\u4f46\u5728\u6cd5\u6c0f\u56ca(bursa) \u548c\u9aa8\u9ad3 (bone marrow) \u4e2d\u7684\u8868\u9054\u4e0d\u52301\uff05\u3002 \u55ae\u682a\u6297\u9ad4EP42\u8b58\u5225CD8\u03b2\u93c8\u3002Mouse Anti-Chicken CD8\u03b2-PE\u6297\u9ad4\uff0c\u5177PE\u87a2\u5149\u6a19\u8a8c\u3002\u514d\u75ab\u539f\u70baChicken splenocytes\u91dd\u5c0dChicken CD8\u03b2\u5177\u7279\u7570\u6027\u3002Isotype\u70baMouse (BALB\/c) IgG2a<\/sub>\u03ba\u3002Isotype Control Mouse IgG2a<\/sub>-PE<\/a>\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u53ca stabilizer\u3002<\/p>\n

\"\"<\/p>\n

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8\u03b2-PE (SB Cat. No. 8280-09) and Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02)<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nEP42<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG2a<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG2a<\/sub>-PE<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nChicken splenocytes<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken CD8\u03b2<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nIn the chicken, the CD8 molecule is present in two forms – (i) a homodimer of two \u03b1 chains and (ii) a heterodimer of an \u03b1 chain and a \u03b2 chain. While the vast majority of CD8+<\/sup>\u00a0cells in the thymus, spleen, and blood of adult chickens express both CD8\u03b1- and CD8\u03b2-chains, a relatively large proportion of the CD8+<\/sup>\u00a0TCR-\u03b3\u03b4 cells in the spleens of embryos and young chicks express only the \u03b1-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+<\/sup>\u00a0T cell populations present in mice are conserved but there is a population of TCR-\u03b3\u03b4 CD8\u03b1\u03b2 cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8\u03b2 chain.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nPE (R-phycoerythrin)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide and a stabilizer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.1 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light; Do not freeze
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,2,4-9<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a02,3<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796481<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737bReferences<\/p>\n

    \n
  1. Tregaskes CA, Kong F, Paramithiotis E, Chen CH, Ratcliffe MJ, Davison TF, et al. Identification and analysis of the expression of CD8\u03b1\u03b2 and CD8\u03b1\u03b1 isoforms in chickens reveals a major TCR-\u03b3\u03b4 CD8\u03b1\u03b2 subset of intestinal intraepithelial lymphocytes. J Immunol. 1995;154:4485-94. (Immunogen, FC, IP)<\/li>\n
  2. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)<\/li>\n
  3. Pielsticker C. Investigations on the humoral and cell-mediated immune response in chicken after inoculation with Campylobacter jejuni strains of human and avian origin [thesis]. Hannover (Germany): University of Veterinary Medicine Hannover; 2013. (IHC-FS)<\/li>\n
  4. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)<\/li>\n
  5. van Ginkel FW, van Santen VL, Gulley SL, Toro H. Infectious bronchitis virus in the chicken Harderian gland and lachrymal fluid: viral load, infectivity, immune cell responses, and effects of viral immunodeficiency. Avian Dis. 2008;52:608-17. (FC)<\/li>\n
  6. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek’s disease virus. Viral Immunol. 2009;22:321-7. (FC)<\/li>\n
  7. Norup LR, Dalgaard TS, Friggens NC, S\u00f8rensen P, Juul-Madsen HR. Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli. Poult Sci. 2009;88:543-53. (FC)<\/li>\n
  8. Petrov P, Motobu M, Salmi J, Uchida T, Vainio O. Novel leukocyte protein, Trojan, differentially expressed during thymocyte development. Mol Immunol. 2010;47:1522-8. (FC)<\/li>\n
  9. Mwangi WN, Smith LP, Baigent SJ, Beal RK, Nair V, Smith AL. Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells. PLoS Pathog. 2011;7(5):e1001337. (FC)<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n
    \u00a0Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nClone<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n<\/thead>\n
    8280-08<\/strong><\/a><\/td>\nMouse Anti-Chicken CD8\u03b2-BIOT<\/strong><\/td>\nEP42<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    8280-02<\/strong><\/a><\/td>\nMouse Anti-Chicken CD8\u03b2-FITC<\/strong><\/td>\nEP42<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    8280-09<\/strong><\/a><\/td>\nMouse Anti-Chicken CD8\u03b2-PE<\/strong><\/td>\nEP42<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8280-01<\/strong><\/a><\/td>\nMouse Anti-Chicken CD8\u03b2-UNLB<\/strong><\/td>\nEP42<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Chicken CD8\u03b2-PE\u6297\u9ad4 | \u8ca8\u865f8280-09\u00a0 \u898f\u683c1.0 mL \u00a0(0. […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2236],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19631"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19631"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19631\/revisions"}],"predecessor-version":[{"id":19632,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19631\/revisions\/19632"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19631"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19631"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19631"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}