{"id":19627,"date":"2019-04-29T16:28:06","date_gmt":"2019-04-29T08:28:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19627"},"modified":"2022-07-20T14:00:07","modified_gmt":"2022-07-20T06:00:07","slug":"mouse-anti-chicken-cd4-fitc%e6%8a%97%e9%ab%94-southernbiotech-sba%e8%b2%a8%e8%99%9f8210-02","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19627","title":{"rendered":"Mouse Anti-Chicken CD4-FITC\u6297\u9ad4 | Southernbiotech SBA\u8ca8\u865f8210-02"},"content":{"rendered":"

Mouse Anti-Chicken CD4-FITC\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>8210-02\u00a0 <\/a><\/span>\u898f\u683c<\/strong>1.0 mL \u00a0(0.5 mg\/mL)<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0CD4\u662fI\u578b\u8de8\u819c\u7cd6\u86cb\u767d (type I transmembrane glycoprotein)\uff0c\u5728\u7d0470\uff05\u7684\u80f8\u817a\u7d30\u80de (thymocytes)\uff0c10\uff05\u7684\u813e\u7d30\u80de (spleen cells)\u548c45\uff05\u7684\u5916\u5468\u8840\u6dcb\u5df4\u7d30\u80de(peripheral blood lymphocytes)\u4e0a\u8868\u9054\uff0c\u4f46\u4e0d\u52301\uff05\u7684\u6cd5\u6c0f\u56ca\u7d30\u80de (spleen cells)\u3002 \u55ae\u682a\u6297\u9ad4CT-4\u6291\u5236PHA\u548cCon A\u8a98\u5c0e\u7684\u813e\u7d30\u80de(splenocytes)\u589e\u6b96\u53cd\u61c9\u548cPWM\u8a98\u5c0e\u7684IL-2\u7522\u751f\u3002 This antibody also reacts with turkey and quail as demonstrated by flow cytometry.\u00a0\u00a0\u00a0Mouse Anti-Chicken CD4-FITC\u6297\u9ad4\uff0c\u5177FITC\u87a2\u5149\u6a19\u8a8c\u3002\u514d\u75ab\u539f\u70baChicken thymocytes and Ig-negative blood leukocytes\u91dd\u5c0dChicken\/Turkey\/Quail\/Pigeon CD4\u5177\u7279\u7570\u6027\u3002Isotype\u70baMouse (BALB\/c) IgG1<\/sub>\u03ba\u3002Isotype Control Mouse IgG1<\/sub>-FITC<\/a>\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n

\"\"\"\"<\/p>\n

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8210-02) and Mouse Anti-Chicken CD3-PE (SB Cat. No. 8200-09).<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nCT-4<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-FITC<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nChicken thymocytes and Ig-negative blood leukocytes<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken\/Turkey\/Quail\/Pigeon CD4<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nCD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10% of spleen cells, and 45% of peripheral blood lymphocytes but less than 1% of bursal cells. The monoclonal antibody CT-4 inhibits both PHA- and Con A-induced proliferative responses of splenocytes and PWM-induced IL-2 production. This antibody also reacts with turkey and quail as demonstrated by flow cytometry.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nFITC (Fluorescein)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,6-19<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a02-4<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a05<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01<\/sup>
\nBlocking \u2013 Reported in literature\u00a01<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796428<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737bReferences<\/strong><\/p>\n

    \n
  1. Chan MM, Chen CH, Ager LL, Cooper MD. Identification of the avian homologues of mammalian CD4 and CD8 antigens. J Immunol. 1988;140:2133-8. (Immunogen, FC, IP, Block)<\/li>\n
  2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)<\/li>\n
  3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. (IHC-FS)<\/li>\n
  4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. (IHC-FS)<\/li>\n
  5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. (IHC-PS)<\/li>\n
  6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8\u03b1 molecule. Poult Sci. 1999;78:1526-31. (FC, Turkey Reactivity)<\/li>\n
  7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. (FC)<\/li>\n
  8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74. (FC, Turkey Reactivity)<\/li>\n
  9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)<\/li>\n
  10. Tyka\u0142owski B, Stenzel T, Mikulski D, Jankowski J, Zdu\u0144czyk Z, Ju\u015bkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7. (FC)<\/li>\n
  11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. (FC)<\/li>\n
  12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137. (FC, Quail Reactivity)<\/li>\n
  13. Revajov\u00e1 V, Slaminkov\u00e1 Z, Gre\u0161\u00e1kov\u00e1 L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)<\/li>\n
  14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. (FC)<\/li>\n
  15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. (FC)<\/li>\n
  16. Laniewski P, Kuczkowski M, Chrz\u0105stek K, Wo\u017aniak A, Wyszy\u0144ska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. (FC)<\/li>\n
  17. Kj\u00e6rup RM, Dalgaard TS, Norup LR, Hamzic E, S\u00f8rensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. (FC)<\/li>\n
  18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. (FC)<\/li>\n
  19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33. (FC, Pigeon Reactivity)<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    \u00a0Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nClone<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n<\/thead>\n
    8210-30<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>\u00a0488<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-31<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>\u00a0647<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-27<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>\u00a0700<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-11<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-APC<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-08<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-BIOT<\/strong><\/td>\nCT-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    8210-02<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-FITC<\/strong><\/td>\nCT-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    8210-26<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-Pacific Blue\u2122<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-17<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-PE\/CY7<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-09<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-PE<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-13<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-SPRD<\/strong><\/td>\nCT-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    8210-01<\/strong><\/a><\/td>\nMouse Anti-Chicken CD4-UNLB<\/strong><\/td>\nCT-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Chicken CD4-FITC\u6297\u9ad4 | \u8ca8\u865f8210-02\u00a0 \u898f\u683c1.0 mL \u00a0(0 […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2237],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19627"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19627"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19627\/revisions"}],"predecessor-version":[{"id":33203,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19627\/revisions\/33203"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19627"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19627"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}