{"id":19545,"date":"2019-04-22T11:44:02","date_gmt":"2019-04-22T03:44:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19545"},"modified":"2025-03-21T14:45:01","modified_gmt":"2025-03-21T06:45:01","slug":"hgl5%e6%b0%b8%e7%94%9f%e5%8c%96%e4%ba%ba%e9%a1%86%e7%b2%92%e7%b4%b0%e8%83%9e-immortalized-human-granulosa-cells-hgl5%e8%b2%a8%e8%99%9ft0650","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19545","title":{"rendered":"HGL5\u6c38\u751f\u5316\u4eba\u9846\u7c92\u7d30\u80de | Immortalized Human Granulosa Cells (HGL5)\u8ca8\u865fT0650"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u85e5\u7269\u7be9\u9078\u5e73\u53f0<\/strong> | <\/strong>\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6c38\u751f\u5316\u4eba\u9846\u7c92\u7d30\u80de\uff08HGL5\uff09Immortalized Human Granulosa Cells (HGL5) |\u00a0\u8ca8\u865fT0650<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u5375\u5de2\u9846\u7c92\u7d30\u80de\uff08Human ovarian granulosa cells , HGL5\uff09\u662f\u96cc\u6fc0\u7d20(estrogen)\u548c\u5b55\u916e\u7522\u751f\u7684\u90e8\u4f4d\uff0c\u4f7f\u5176\u6210\u70ba\u7814\u7a76\u985e\u56fa\u9187\u751f\u7269\u5408\u6210(steroid biosynthesis)\uff0c\u985e\u56fa\u9187\u4ee3\u8b1d\u9176(steroid-metabolizing enzymes)\uff0c\u7279\u5b9a\u751f\u9577\u56e0\u5b50(growth factors)\u548c\u4fc3\u6027\u817a\u6fc0\u7d20(gonadotropins)\u7684\u95dc\u9375\u3002\u6c38\u751f\u5316\u4eba\u9846\u7c92\u7d30\u80de\uff08Human ovarian granulosa cells , HGL5\uff09\u8868\u73fe\u51fa\u8207\u539f\u4ee3\u5375\u5de2\u9846\u7c92\u7d30\u80de(primary ovarian granulosa cells)\u4e00\u81f4\u7684\u54c1\u8cea; \u9019\u5305\u62ec\u97ff\u61c9\u86cb\u767d\u6fc0\u9176-A\u6d3b\u5316(protein kinase-A activation)\u7684\u7d30\u80de\u56de\u7e2e(cell retraction)\uff0c\u7522\u751f\u9ec3\u9ad4\u916e(progesterone) \u548c\u96cc\u4e8c\u9187(estradiol)\u7684\u80fd\u529b\uff0c\u4ee5\u53ca\u96cc\u4e8c\u9187(estradiol)\u7522\u751f\u7684\u9176\uff0cP450arom\u3002 \u6240\u6709\u9019\u4e9b\u7279\u5fb5\u4f7f\u5f97\u6c38\u751f\u5316\u4eba\u9846\u7c92\u7d30\u80de\u682a(Immortalized Human Granulosa Cell Line)\u6210\u70ba\u7814\u7a76\u8207\u985e\u56fa\u9187\u751f\u7269\u5408\u6210\u548c\u4ee3\u8b1d\u76f8\u95dc\u7684\u6a5f\u5236\u7684\u6709\u5438\u5f15\u529b\u7684\u6a21\u578b\uff0c\u4ee5\u53ca\u5176\u4ed6\u9014\u5f91\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nOvaries<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nOther<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\n20,000 cells\/cm2<\/sup><\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and transduction with retroviruses carrying human papilloma virus strain 16 (HPV16) oncogenes E6\/E7<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nHuman ovarian granulosa cells are the sites of estrogen and progesterone production, making them critical in studying steroid biosynthesis, steroid-metabolizing enzymes, particular growth factors, and gonadotropins. The Immortalized Human Granulosa Cells (HGL5) exhibits qualities consistent with primary ovarian granulosa cells; this includes cell retraction in response to protein kinase-A activation, the ability to produce progesterone and estradiol, and the enzyme integral to estradiol production, P450arom. All of these characteristics make the Immortalized Human Granulosa Cell Line an attractive model to study mechanisms relating to steroid biosynthesis and metabolism, in addition to other pathways.<\/td>\n<\/tr>\n
Depositor Information<\/strong><\/td>\nUT Southwestern<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Propagation Requirements<\/strong><\/td>\nUse of PriCoat\u2122 T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.<\/strong>\u00a0The base medium for this cell line is Prigrow IV medium available at\u00a0abm<\/strong>, Cat. No.TM004<\/a>. To make the complete growth medium, add the following components to the base medium: 2% Cosmic Calf Serum (Hyclone), 2% Ultroser G (Pall Corporation), 1% ITS plus (Zen-Bio), and Penicillin\/Streptomycin Solution (G255<\/a>) to a final concentration of 1%
\nCarbon dioxide (CO2<\/sub>): 5%, Temperature: 37.0\u00b0C.Low serum media for experiments:<\/strong>\u00a0The base medium for this cell line is Prigrow IV medium available at\u00a0abm<\/strong>, Cat. No.TM004<\/a>. To make the complete growth medium, add the following components to the base medium: 0.2% Ultroser SF (Pall Corporation), 1% ITS plus (Zen-Bio), and Penicillin\/Streptomycin Solution (G255<\/a>) to a final concentration of 1%
\nCarbon dioxide (CO2<\/sub>): 5%, Temperature: 37.0\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Immortalized Human Granulosa Cells (HGL5) \u7d30\u80de\u682a | \u4e0d\u6747\u65b9\u6cd5 : Serial passaging and transduction with retroviruses carrying human papilloma virus strain 16 (HPV16) oncogenes E6\/E7 | \u5668\u5b98\u4f86\u6e90Ovaries | \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIV (<\/u>\u8ca8\u865f<\/u>\u00a0<\/u>TM004<\/a>)<\/u>\u3002\u70ba\u4e86\u88fd\u5099\u5b8c\u6574\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4e0b\u5217\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1aCosmic Calf Serum (Hyclone)\u81f3\u6700\u7d42\u6fc3\u5ea62%\uff0c2% Ultroser G (Pall Corporation)\uff0c1% ITS plus (Zen-Bio)\uff0c\u548cPenicillin\/Streptomycin\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\u6eb6\u6db2\uff08G255\uff09\u81f3\u6700\u7d42\u6fc3\u5ea6\u70ba1\uff05\u3002\u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002<\/p>\n

\u7528\u65bc\u5be6\u9a57\u7684\u4f4e\u8840\u6e05\u57f9\u990a\u57fa\uff1a\u8a72\u7d30\u80de\u682a\u7684\u57fa\u790e\u57f9\u990a\u57fa\u662fPrigrow IV\u57f9\u990a\u57fa\uff0c\u53ef\u5728abm\uff0cCat No.TM004\u3002 \u70ba\u4e86\u88fd\u5099\u5b8c\u6574\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4ee5\u4e0b\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1a0.2\uff05Ultroser SF\uff08Pall Corporation\uff09\uff0c1\uff05ITS plus\uff08Zen-Bio\uff09\u548c\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\u6eb6\u6db2\uff08G255\uff09\u81f3\u7d42\u6fc3\u5ea6\u70ba1\uff05\u3002\u7d30\u80de\u682a\u9451\u5b9a(Quality Control)<\/strong>\uff081\uff09\u901a\u904ePCR\u5b9a\u91cf\u7684\u75c5\u6bd2\u57fa\u56e0\u7684\u8868\u9054; \uff082\uff09\u7d93\u7531forskolin\u3001cAMP\u3001\u5375\u6ce1\u523a\u6fc0\u7d20\uff08follicle-stimulating hormone , FSH\uff09\u6216\u4fc3\u9ec3\u9ad4\u6fc0\u7d20\uff08luteinizing hormone ,LH\uff09\u8655\u7406\u8a98\u5c0e\u5f8c\u6e2c\u5b9acAMP\u3001\u5b55\u916e (progesterone) \u548c\u96cc\u4e8c\u9187\u7684\u7522\u751f \uff083\uff09\u901a\u904eNorthern\u5370\u8de1 (North blot analysis) \u5206\u6790\u8868\u5fb5p450arom\u8abf\u7bc0\u3002<\/p>\n

\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

Decomplement<\/strong>\u52a0\u71b1\u975e\u6d3b\u5316<\/strong>FBS<\/strong>\uff1a<\/strong><\/p>\n

1.\u8b93FBS\u74f6\u57284\u00b0C\u4e0b\u5b8c\u5168\u89e3\u51cd\u904e\u591c\u3002<\/p>\n

2.\u5c07FBS\u74f6\u7f6e\u65bc56\u00b0C\u7684\u6c34\u6d74\u4e2d30\u5206\u9418\u3002<\/p>\n

Decomplemented FBS\u53ef\u4ee5\u5728-20\u00b0C\u4e0b\u9577\u671f\u4fdd\u5b58\u3002\u907f\u514d\u983b\u7e41\u7684\u51cd\u878d\u5faa\u74b0\u3002\u6bcf2-3\u5929\u66f4\u63db\u4e00\u6b21\u5b8c\u6574\u7684\u5a92\u9ad4\u3002\u4e0d\u8981\u8b93\u4ecb\u8cea\u984f\u8272\u8b8a\u70ba\u9ec3\u8272\u3002<\/p>\n

Thawing<\/strong>\u7d30\u80de\u89e3\u51cd\u65b9\u6cd5\uff1a<\/strong>(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f<\/strong>)<\/strong><\/p>\n

1.\u572837\u2103\u6c34\u6d74\u4e2d\u89e3\u51cd\u5c0f\u74f6\uff0c\u76f4\u5230\u53ea\u6709\u4e00\u5c0f\u584a\u51b0\u584a\u3002\u91cd\u8981\u7684\u662f\u4e0d\u8981\u904e\u5ea6\u89e3\u51cd\u51b7\u51cd\u7ba1\uff0c\u56e0\u70baDMSO\u7684\u5b58\u5728\u5c0d\u7d30\u80de\u6709\u6bd2\u3002<\/p>\n

2.\u5c07\u7d30\u80de\u8f49\u79fb\u5230\u542b\u67095ml pre-warmed complete media \u4e4b15ml\u7ba1\u4e2d\u3002<\/p>\n

3.\u5c07\u7d30\u80de\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

4.\u5c0f\u5fc3\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u6253\u64fe\u7d30\u80de\u6c89\u6fb1\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u57281ml\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

5.\u5c07\u7d30\u80de\u57f9\u990a\u572820,000 cells\/cm2<\/sup>\u3002<\/p>\n

Subculture<\/strong>\u7d30\u80de\u7e7c\u4ee3\u65b9\u6cd5<\/strong>:<\/strong>\u00a0(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c<\/strong>)<\/strong><\/p>\n

1.\u7576\u7d30\u80de70-90\uff05 confluency\u6642\uff0c\u5efa\u8b70\u7e7c\u4ee3\u57f9\u990a\u3002<\/p>\n

2.Aspirate old media\u3002\u61f8\u6d6e\u6db2\u4e2d\u7684\u4e00\u4e9b\u7d30\u80de\u4ecd\u7136\u662f\u6d3b\u7d30\u80de\u3002\u6216\u8005\uff0c\u4e5f\u53ef\u4ee5\u6536\u96c6\u542b\u6709\u61f8\u6d6e\u7d30\u80de\u7684\u4e0a\u6e05\u6db2\u4e26\u96e2\u5fc3\uff08Skip to Step 6 in protocol\uff09\u3002<\/p>\n

3.\u4ee51X\u7121\u83ccPBS\u548c\u7a00\u91cb0.25\uff05\u80f0\u86cb\u767d\u9176-EDTA\uff08TM051\uff09\u81f3\u6700\u7d42\u6fc3\u5ea6\u70ba0.05%\u3002Dilute Trypsin-EDTA (TM050) to final concentration of 0.05%<\/p>\n

4.\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u7d30\u80de3-5\u5206\u9418\u4e26\u652a\u62cc\u57f9\u990a\u5bb9\u5668\u76f4\u81f390\uff05\u7684\u7d30\u80de\u812b\u843d\u3002<\/p>\n

5.\u4e2d\u548c\u80f0\u86cb\u767d\u9176:\u6dfb\u52a0\u7b49\u91cf\u4e4btrypsin + PBS\u3002<\/p>\n

6.\u6536\u96c6\u7d30\u80de\u4e26\u4ee5200xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

7.\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

8.\u5efa\u8b70split ratio\u6bd4\u4e0d\u8d85\u904e1\uff1a3\u3002<\/p>\n

Freezing<\/strong>\u7d30\u80de\u51cd\u7d50\uff1a<\/strong>(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f<\/strong>)<\/strong><\/p>\n

Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.<\/p>\n

\u51b7\u51cd\u6db2\u914d\u65b9(Freeze Medium): \u542b\u670920\uff05FBS\u548c10\uff05DMSO\u7684\u5b8c\u5168\u751f\u9577\u57f9\u990a\u57fa\u3002 \u7d30\u80de\u5132\u5b58\u6eab\u5ea6( Storage Temperature): \u6db2\u6c2e\u6c23\u76f8Liquid nitrogen vapour phase\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u85e5\u7269\u7be9\u9078\u5e73\u53f0 | \u7d30\u80de\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5316\u4eba\u9846\u7c92\u7d30\u80de\uff08HGL […]<\/p>\n","protected":false},"author":3,"featured_media":19546,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[2245],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19545"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19545"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19545\/revisions"}],"predecessor-version":[{"id":50823,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19545\/revisions\/50823"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19546"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19545"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19545"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}