{"id":19506,"date":"2019-04-17T17:33:10","date_gmt":"2019-04-17T09:33:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19506"},"modified":"2025-12-31T14:56:46","modified_gmt":"2025-12-31T06:56:46","slug":"nadp-nadph%e5%ae%9a%e9%87%8f%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk347-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19506","title":{"rendered":"NADP \/ NADPH\u5b9a\u91cf\u8a66\u5291\u76d2 | Biovision\u8ca8\u865fK347-100 (ABCAM ab65349)"},"content":{"rendered":"

NADP \/ NADPH\u5b9a\u91cf\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K347-100 (ABCAM ab65349)<\/a><\/span><\/p>\n

NADP\/NADPH Quantitation Colorimetric Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u7159\u9170\u80fa\u6838\u82f7\u9178 (nicotinamide nucleotides) \u7684\u6e2c\u5b9a\u5728\u7d30\u80de\u6216\u7d44\u7e54\u7684\u80fd\u91cf\u8f49\u5316\u548c\u6c27\u5316\u9084\u539f\u72c0\u614b\u7684\u7814\u7a76\u4e2d\u6301\u7e8c\u53d7\u5230\u95dc\u6ce8\u3002 NADP \/ NADPH\u5b9a\u91cf\u8a66\u5291\u76d2\u70ba\u7d30\u80de\u5167\u6838\u82f7\u9178\u7684\u9748\u654f\u6aa2\u6e2c\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u5de5\u5177\uff1aNADP\uff0cNADPH\u53ca\u5176\u6bd4\u4f8b\u3002 \u7cfb\u7d71\u4e2d\u7684\u9176\u7279\u7570\u6027\u8b58\u5225\u9176\u5faa\u74b0\u53cd\u61c9\u4e2d\u7684NADP \/ NADPH\uff08\u5b83\u4e0d\u8b58\u5225NAD + \/ NADH\uff09\u3002 \u7121\u9700\u5f9e\u6a23\u54c1\u6df7\u5408\u7269\u4e2d\u7d14\u5316NADP \/ NADPH\u3002 \u9176\u5faa\u74b0\u53cd\u61c9\u986f\u8457\u63d0\u9ad8\u4e86\u6aa2\u6e2c\u9748\u654f\u5ea6\u3002 \u53ef\u4ee5\u4f7f\u7528plate reader\u5728OD450nm\u4e0b\u5b9a\u91cf\u3002<\/p>\n

\"\"\"Functional<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK347-100 (ABCAM ab65349)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nConvenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~2 hours
\n\u2022 Fast and convenient
\n\u2022 Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio
\n\u2022 Enzymes in the system specifically recognize NADP\/NADPH in an enzyme cycling reaction (It does not recognize NAD\u1da7\/NADH)<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 NADP\/NADPH Extraction Buffer
\n\u2022 NADP Cycling Buffer
\n\u2022 NADP Cycling Enzyme Mix
\n\u2022 NADPH Developer
\n\u2022 Stop Solution
\n\u2022 NADPH Standard (MW:833.36)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Hu J et al., (2017) Tumor grade related expression of neuroglobin is negatively regulated by PPAR\u03b3 and confers antioxidant activity in glioma progression, Redox Biol, 2017, 12:682-689<\/p>\n

Wu J et al., (2017) Redox imbalance and mitochondrial abnormalities in the diabetic lung, Redox Biol, 2017, 11:51-59<\/p>\n

Chaman et al., ERK2-Pyruvate Kinase Axis Permits Phorbol 12-Myristate 13-Acetate-induced Megakaryocyte Differentiation in K562 Cells.\u00a0 J. Biol. Chem., Sep 2015; 290: 23803 – 23815.<\/p>\n

Zhao et al., Regenerative Neurogenesis After Ischemic Stroke Promoted by Nicotinamide Phosphoribosyltransferase\u2013Nicotinamide Adenine Dinucleotide Cascade. Stroke, Jul 2015; 46: 1966 – 1974.<\/p>\n

Li et al., Mitofusin-2-mediated tethering of mitochondria and endoplasmic reticulum promotes cell cycle arrest of vascular smooth muscle cells in G0\/G1 phase. Acta Biochim Biophys Sin, Jun 2015; 47: 441 – 450.<\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

NADP \/ NADPH\u5b9a\u91cf\u8a66\u5291\u76d2 | \u8ca8\u865fK347-100 (ABCAM ab65349) NADP\/NAD […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,3],"tags":[2252],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19506"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19506"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19506\/revisions"}],"predecessor-version":[{"id":54331,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19506\/revisions\/54331"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19506"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19506"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19506"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}