{"id":19488,"date":"2019-04-16T12:11:44","date_gmt":"2019-04-16T04:11:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19488"},"modified":"2025-03-21T14:45:21","modified_gmt":"2025-03-21T06:45:21","slug":"%e5%88%9d%e4%bb%a3%e8%83%b0%e8%85%ba%e6%98%9f%e7%8b%80%e7%b4%b0%e8%83%9e-human-primary-pancreatic-stellate-cells-%e8%b2%a8%e8%99%9ft4215","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19488","title":{"rendered":"\u521d\u4ee3\u80f0\u817a\u661f\u72c0\u7d30\u80de |Human Primary Pancreatic Stellate Cells (\u8ca8\u865fT4215)"},"content":{"rendered":"

A<\/strong>bmgood <\/strong>\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (Primary cell)<\/strong> |<\/strong>\u8ca8\u865f<\/strong>T4215\u00a0\u00a0 106 <\/sup>cells \/ 1.0 ml<\/strong><\/p>\n

\u521d\u4ee3\u80f0\u817a\u661f\u72c0\u7d30\u80deHuman Primary Pancreatic Stellate Cells\u00a0<\/strong>(<\/strong>\u8ca8\u865f<\/strong>T4215<\/strong>)<\/strong><\/a><\/p>\n

Human Primary Pancreatic Stellate Cells<\/strong>\u7d30\u80de\u751f\u9577\u7279\u6027Adherent \/ \u5f62\u614bMyofibroblast-like | \u4f86\u6e90Pancreas | \u5efa\u8b70\u57f9\u990a\u5bc6\u5ea6\u00a015,000-20,000 cells\/cm^2
\n| T4215\u7684\u500d\u589e\u6642\u9593\u7d04\u70ba48-72\u5c0f\u6642\u3002\u8a72\u7d30\u80de\u7cfb\u7684\u5efa\u8b70\u7e7c\u4ee3\u6bd4\u4f8b\u70ba1\uff1a2\uff0c\u6211\u5011\u5efa\u8b70\u5728\u7d30\u80de\u9054\u5230\u7d0480\uff05\u6eff\u6642\uff0c\u518d\u9032\u884c\u7d30\u80de\u7e7c\u4ee3\u57f9\u990a\uff0c Medium\u61c9\u6bcf2-3\u5929\u66f4\u65b0\u4e00\u6b21\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2Prigrow X series (TM4215<\/a><\/span>)\u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nPancreas<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nMyofibroblast-like<\/td>\n<\/tr>\n
Disease<\/strong><\/td>\nNormal<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Cell Type Characterization<\/strong><\/td>\n\u00a0\"\"<\/td>\n<\/tr>\n
Image<\/strong><\/td>\n\u00a0\"\"<\/td>\n<\/tr>\n
Propagation Requirements<\/strong><\/td>\nUse of PriCoat\u2122 T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.<\/strong>\u00a0The base medium for this cell line is Prigrow X series medium available at\u00a0abm<\/strong>, Cat. No.\u00a0TM4215<\/a>.
\nCarbon dioxide (CO2<\/sub>): 5%, Temperature: 37.0\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a>PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2<\/a><\/span><\/p>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

Huo, Z\u00a0et al<\/i>. “Atorvastatin enhances endothelial adherens junctions through promoting VE-PTP gene transcription and reducing VE-cadherin-Y731 phosphorylation.” Vascul Pharmacol. : (2018).\u00a0DOI: 10.1016\/j.vph.2018.06.003<\/a>.\u00a0PubMed: 29894844<\/a>. Application: Vascular homeostasis.<\/p>\n

Hybertson, B.M.\u00a0et al<\/i>. “Electrochemical plasma activated aqueous chemotherapeutics.” US Patent : (2017).<\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood \u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell) |\u8ca8\u865fT4215\u00a0\u00a0 106 cells \/ […]<\/p>\n","protected":false},"author":3,"featured_media":19489,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[2255],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19488"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19488"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19488\/revisions"}],"predecessor-version":[{"id":50824,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19488\/revisions\/50824"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19489"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19488"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19488"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}