{"id":19472,"date":"2019-04-12T16:39:31","date_gmt":"2019-04-12T08:39:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19472"},"modified":"2022-11-14T15:47:24","modified_gmt":"2022-11-14T07:47:24","slug":"goat-anti-mouse-igg-human-ads-fitc%e6%8a%97%e9%ab%94-southernbiotech%e8%b2%a8%e8%99%9f2015-02","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19472","title":{"rendered":"Goat Anti-Mouse IgG, Human ads-FITC\u6297\u9ad4 | Southernbiotech\u8ca8\u865f1030-02"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=19472\">Goat Anti-Mouse IgG, Human ads-FITC\u6297\u9ad4<strong> | <\/strong><strong>\u8ca8\u865f<\/strong><strong>1030-02 \u00a0<\/strong><strong>\u898f\u683c<\/strong>1.0 mL \u00a0(1.0 mg\/mL)<\/a><\/span><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u00a0Goat Anti-Mouse IgG, Human ads-FITC\u6297\u9ad4\uff0c\u5177FITC\u87a2\u5149\u6a19\u8a8c\u3002\u91dd\u5c0dmouse IgG\u7684\u91cd\u934amouse IgG<sub>1<\/sub>, IgG<sub>2a<\/sub>, IgG<sub>2b<\/sub>, IgG<sub>2c<\/sub>, and IgG<sub>3<\/sub>\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70ba\u5c71\u7f8ahyperimmunized with mouse IgG\u3002Isotype\u70baGoat IgG\u3002Isotype Control <a href=\"https:\/\/www.southernbiotech.com\/?catno=0109-02&amp;type=Polyclonal\">Goat IgG-FITC<\/a>\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.southernbiotech.com\/ImageUtil.aspx?img=\/ProductImages2\/1030-02.02.jpg&amp;op=sb&amp;h=500&amp;w=500\" alt=\"\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/11\/1030-02.jpeg\" alt=\"\" width=\"444\" height=\"294\" \/><\/p>\n<p>Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG, Human ads-FITC (SB Cat. No. 1030-02) and DAPI.<\/p>\n<table width=\"580\">\n<tbody>\n<tr>\n<td width=\"145\"><strong>Specificity<\/strong><\/td>\n<td>Reacts with the heavy chains of mouse IgG<sub>1<\/sub>, IgG<sub>2a<\/sub>, IgG<sub>2b<\/sub>, IgG<sub>2c<\/sub>, and IgG<sub>3<\/sub><\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Source<\/strong><\/td>\n<td>Pooled antisera from goats hyperimmunized with mouse IgG<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Cross Adsorption<\/strong><\/td>\n<td>Mouse IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Purification<\/strong><\/td>\n<td>Affinity chromatography on mouse IgG covalently linked to agarose<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Isotype<\/strong><\/td>\n<td>Goat IgG<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Isotype Control<\/strong><\/td>\n<td><a href=\"https:\/\/www.southernbiotech.com\/?catno=0109-02&amp;type=Polyclonal\">Goat IgG-FITC<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Format\/Conjugate<\/strong><\/td>\n<td>FITC (Fluorescein)<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Buffer Formulation<\/strong><\/td>\n<td>Phosphate buffered saline containing 0.1% sodium azide<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Concentration<\/strong><\/td>\n<td>1.0 mg\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Volume<\/strong><\/td>\n<td>1.0 mL<\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Storage &amp; Handling<\/strong><\/td>\n<td>2-8\u00b0C; Avoid exposure to light<br \/>\nPlease refer to product specific\u00a0<a href=\"https:\/\/www.southernbiotech.com\/Msds\/1030-02.pdf\">SDS<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"145\"><strong>Applications<\/strong><\/td>\n<td>\n<p>Quality tested applications for relevant formats include &#8211;<\/p>\n<p>ELISA\u00a0<sup>1-10<\/sup><br \/>\nFLISA<br \/>\nFlow Cytometry\u00a0<sup>5,11-13<\/sup><\/p>\n<p>Other referenced applications for relevant formats include &#8211;<\/p>\n<p>ELISpot\u00a0<sup>1,3-6<\/sup><br \/>\nImmunohistochemistry-Frozen Sections\u00a0<sup>2,14,15<\/sup><br \/>\nImmunohistochemistry-Paraffin Sections\u00a0<sup>16-18<\/sup><br \/>\nImmunocytochemistry\u00a0<sup>19-22<\/sup><br \/>\nWestern Blot\u00a0<sup>7,21,23-25<\/sup><br \/>\nMultiplex\u00a0<sup>26,27<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<\/strong><strong>References<\/strong><\/p>\n<ol>\n<li>van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-\u03b21 knockout mice. J Immunol. 1999;163:1951-7. (ELISA, ELISPOT)<\/li>\n<li>Dunn LA, Upcroft JA, Fowler EV, Matthews BS, Upcroft P. Orally administered Giardia duodenalis extracts enhance an antigen-specific antibody response. Infect Immun. 2001;69:6503-10. (ELISA, IHC-FS)<\/li>\n<li>Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-\u03b3 production. Int J Exp Path. 2002;83:121-32. (ELISA, ELISPOT)<\/li>\n<li>Hovden A, Cox RJ, Haaheim LR. Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB\/c mice. Scand J Immunol. 2005;62:36-44. (ELISA, ELISPOT)<\/li>\n<li>Conway KL, Kuballa P, Khor B, Zhang M, Shi HN, Virgin HW, et al. ATG5 regulates plasma cell differentiation. Autophagy. 2013;9:528-37. (ELISA, ELISPOT, FC)<\/li>\n<li>Koutsonanos DG, Esser ES, McMaster SR, Kalluri P, Lee J, Prausnitz MR, et al. Enhanced immune responses by skin vaccination with influenza subunit vaccine in young hosts. Vaccine. 2015;33:4675-82. (ELISA, ELISPOT)<\/li>\n<li>Yolken RH, Jones-Brando L, Dunigan DD, Kannan G, Dickerson F, Severance E, et al. Chlorovirus ATCV-1 is part of the human oropharyngeal virome and is associated with changes in cognitive functions in humans and mice. Proc Natl Acad Sci USA. 2014;111:16106-11. (ELISA, WB)<\/li>\n<li>Zhou Y, Maharaj PD, Mallajosyula JK, McCormick AA, Kearney CM. In planta production of flock house virus transencapsidated RNA and its potential use as a vaccine. Mol Biotechnol. 2015;57:325-36. (ELISA)<\/li>\n<li>Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)<\/li>\n<li>Schubert RD, Hu Y, Kumar G, Szeto S, Abraham P, Winderl J, et al. IFN-\u03b2 treatment requires B cells for efficacy in neuroautoimmunity. J Immunol. 2015;194:2110-6. (ELISA)<\/li>\n<li>Chen J, Yin H, Xu J, Wang Q, Edelblum KL, Sciammas R, et al. Reversing endogenous alloreactive B cell GC responses with anti-CD154 or CTLA-4Ig. Am J Transplant. 2013;13:2280-92. (FC)<\/li>\n<li>Maxwell P, Melendez-Rodr\u00edguez F, Matchett KB, Aragones J, Ben-Califa N, Jaekel H, et al. Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol. 2015;168:429-42. (FC)<\/li>\n<li>Brady AM, Spencer BL, Falsey AR, Nahm MH. Blood collection tubes influence serum ficolin-1 and ficolin-2 levels. Clin Vaccine Immunol. 2014;21:51-5. (FC)<\/li>\n<li>Csencsits K, Burrell BE, Lu G, Eichwald EJ, Stahl GL, Bishop DK. The classical complement pathway in transplantation: unanticipated protective effects of C1q and role in inductive antibody therapy. Am J Transplant. 2008;8:1622-30. (IHC-FS)<\/li>\n<li>Sic H, Kraus H, Madl J, Flittner K, von M\u00fcnchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS)<\/li>\n<li>Zheng P, Zhao YX, Zhang AD, Kang C, Chen HC, Jin ML. Pathologic analysis of the brain from Streptococcus suis type 2 experimentally infected pigs. Vet Pathol. 2009;46:531-5. (IHC-PS)<\/li>\n<li>McPhee CG, Sproule TJ, Shin D, Bubier JA, Schott WH, Steinbuck MP, et al. MHC class I family proteins retard systemic lupus erythematosus autoimmunity and B cell lymphomagenesis. J Immunol. 2011;187:4695-704. (IHC-PS)<\/li>\n<li>Vujasinovic T, Pribic J, Kanjer K, Milosevic NT, Tomasevic Z, Milovanovic Z, et al. Gray-level co-occurrence matrix texture analysis of breast tumor images in prognosis of distant metastasis risk. Microsc Microanal. 2015;21:646-54. (IHC-PS)<\/li>\n<li>Sj\u00f6berg M, Garoff H. Interactions between the transmembrane segments of the alphavirus E1 and E2 proteins play a role in virus budding and fusion. J Virol. 2003;77:3441-50. (ICC)<\/li>\n<li>Roberts MS, Woods AJ, Dale TC, van der Sluijs P, Norman JC. Protein kinase B\/Akt acts via glycogen synthase kinase 3 to regulate recycling of \u03b1v\u03b23 and \u03b15\u03b21 integrins. Mol Cell Biol. 2004;24:1505-15. (ICC)<\/li>\n<li>Ma Y, Yates J, Liang Y, Lemon SM, Yi M. NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly. J Virol. 2008;82:7624-39. (ICC, WB)<\/li>\n<li>Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)<\/li>\n<li>Friedman J, Alam SM, Shen X, Xia S, Stewart S, Anasti K, et al. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648. (WB)<\/li>\n<li>Bender BJ, Coen DM, Strang BL. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol. 2014;88:11738-47. (WB)<\/li>\n<li>Hethorn WR, Ciarlone SL, Filonova I, Rogers JT, Aguirre D, Ramirez RA, et al. Reelin supplementation recovers synaptic plasticity and cognitive deficits in a mouse model for Angelman syndrome. Eur J Neurosci. 2015;41:1372-80. (WB)<\/li>\n<li>Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (Multiplex)<\/li>\n<li>Brummelman J, Helm K, Hamstra H, van der Ley P, Boog CJ, Han WG, et al. Modulation of the CD4+ T cell response after acellular pertussis vaccination in the presence of TLR4 ligation. Vaccine. 2015;33:1483-91. (Multiplex)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Goat Anti-Mouse IgG, Human ads-FITC\u6297\u9ad4 | \u8ca8\u865f1030-02 \u00a0\u898f\u683c1. [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4542,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[1940,2257],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19472"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19472"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19472\/revisions"}],"predecessor-version":[{"id":36025,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19472\/revisions\/36025"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4542"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19472"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19472"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19472"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}