{"id":19468,"date":"2019-04-11T17:23:23","date_gmt":"2019-04-11T09:23:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19468"},"modified":"2022-07-20T14:03:04","modified_gmt":"2022-07-20T06:03:04","slug":"mouse-igg-unlb-southernbiotech%e8%b2%a8%e8%99%9f0107-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19468","title":{"rendered":"Mouse IgG-UNLB | Southernbiotech\u8ca8\u865f0107-01"},"content":{"rendered":"

\"\"<\/p>\n

Mouse IgG-UNLB | <\/strong>\u8ca8\u865f<\/strong>0107-01 \u00a0<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Goat Anti-Human IgG(H+L)-FITC \u6297\u9ad4\uff0c\u4e0d\u5177\u6a19\u8a8c\u3002\u4f86\u6e90\u70baNormal mouse serum\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Borate buffered saline, pH 8.2\u30025.0 mg\/mL<\/p>\n

\"\"<\/p>\n

ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Source<\/strong><\/td>\nNormal mouse serum<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n5.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n2.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

Flow Cytometry\u00a07-10<\/sup>
\nELISA\u00a01-6<\/sup>
\nFLISA<\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

Immunohistochemistry-Paraffin Sections\u00a011<\/sup>
\nImmunocytochemistry\u00a012<\/sup>
\nWestern Blot\u00a013,14<\/sup>
\nImmunoprecipitation\u00a015,16<\/sup>
\nChromatin Immunoprecipitation\u00a017,18<\/sup>
\nBlocking\u00a019,20<\/sup>
\nIn vitro<\/em>\u00a0Control\u00a022-25<\/sup>
\nIn vivo<\/em>\u00a0Control\u00a02,9,21<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2732898<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u5f15\u7528\u6587\u737bReferences<\/strong><\/p>\n

    \n
  1. Kuklin N, Daheshia M, Karem K, Manickan E, Rouse BT. Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization. J Virol. 1997;71:3138-45. (ELISA)<\/li>\n
  2. Cupples AL. Addition of an anti-DNA antibody enhances plasmid DNA vaccination against herpes simplex virus [thesis]. Knoxville (USA): University of Tennessee; 2004. (ELISA, in vivo control)<\/li>\n
  3. Genestier L, Taillardet M, Mondiere P, Gheit H, Bella C, Defrance T. TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses. J Immunol. 2007;178:7779-86. (ELISA)<\/li>\n
  4. Wang Y, Diamond B. B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice. J Clin Invest. 2008;118:2896-907. (ELISA)<\/li>\n
  5. Szymczak WA, Davis MJ, Lundy SK, Dufaud C, Olszewski M, Pirofski L. X-linked immunodeficient mice exhibit enhanced susceptibility to Cryptococcus neoformans infection. Mbio. 2013;4(4): e00265-13. (ELISA)<\/li>\n
  6. Mann JF, McKay PF, Fiserova A, Klein K, Cope A, Rogers P, et al. Enhanced immunogenicity of an HIV-1 DNA vaccine delivered with electroporation via combined intramuscular and intradermal routes. J Virol. 2014;88:6959-69. (ELISA)<\/li>\n
  7. Nataf S, Davoust N, Ames RS, Barnum SR. Human T cells express the C5a receptor and are chemoattracted to C5a. J Immunol. 1999;162:4018-23. (FC)<\/li>\n
  8. Butler JE, Moore MB, Presnell SR, Chan H, Chalupny NJ, Lutz CT. Proteasome regulation of ULBP1 transcription. J Immunol. 2009;182:6600-9. (FC)<\/li>\n
  9. O’Neill AF, Dearling JL, Wang Y, Tupper T, Sun Y, Aster JC, et al. Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody. Clin Cancer Res. 2014;20:678-87. (FC, in vivo control)<\/li>\n
  10. Silvescu CI, Sackstein R. G-CSF induces membrane expression of a myeloperoxidase glycovariant that operates as an E-selectin ligand on human myeloid cells. Proc Natl Acad Sci USA. 2014;111:10696-701. (FC)<\/li>\n
  11. Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (IHC-PS)<\/li>\n
  12. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)<\/li>\n
  13. Lintomen L, Elsas MI, Maximiano ES, de Paula Neto HA, Joseph D, Vargaftig BB, et al. Allergenic sensitization prevents upregulation of haemopoiesis by cyclo-oxygenase inhibitors in mice. Br J Pharmacol. 2002;135:1315-23. (WB)<\/li>\n
  14. Xu J, Cui G, Esmailian F, Plunkett M, Marelli D, Ardehali A, et al. Atrial extracellular matrix remodeling and the maintenance of atrial fibrillation. Circulation. 2004;109:363-8. (WB)<\/li>\n
  15. Inagaki T, Iwaski S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10\/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77. (IP)<\/li>\n
  16. Li G, Nguyen CC, Ryckman BJ, Britt WJ, Kamil JP. A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism. Proc Natl Acad Sci USA. 2015;112:4471-6. (IP)<\/li>\n
  17. Breslin MB, Wang H, Pierce A, Aucoin R, Lan MS. Neurogenin 3 recruits CBP co-activator to facilitate histone H3\/H4 acetylation in the target gene INSM1. FEBS Lett. 2007;581:949-54. (ChIP)<\/li>\n
  18. Tao Y, Sharif N, Zeng B, Cai Y, Guo Y. Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3. Int J Clin Exp Pathol. 2015;8:12564-70. (ChIP)<\/li>\n
  19. B\u00fchring H, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007;1106:262-71. (Block)<\/li>\n
  20. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (Block)<\/li>\n
  21. Arumugam T, Deng D, Bover L, Wang H, Logsdon CD, Ramachandran V. New blocking antibodies against novel AGR2-C4.4A pathway reduce growth and metastasis of pancreatic tumors and increase survival in mice. Mol Cancer Ther. 2015;14:941-51. (In vivo control)<\/li>\n
  22. Nakajima H, Samaridis J, Angman L, Colonna M. Cutting edge: Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor \u03b3-chain. J Immunol. 1999;162:5-8. (In vitro control)<\/li>\n
  23. Milbauer LC, Enenstein JA, Roney M, Solovey A, Bodempudi V, Nichols TC, et al. Blood outgrowth endothelial cell migration and trapping in vivo: a window into gene therapy. Transl Res. 2009;153:179-89. (In vitro control)<\/li>\n
  24. Gibb DR, Shikh ME, Kang D, Rowe WJ, El Sayed R, Cichy J, et al. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J Exp Med. 2010;207:623-35. (In vitro control)<\/li>\n
  25. Xu G, Wen X, Hong Y, Du H, Zhang X, Song J, et al. An anti-transferrin receptor antibody enhanced the growth inhibitory effects of chemotherapeutic drugs on human glioma cells. Int Immunopharmacol. 2011;11:1844-9. (In vitro control)<\/li>\n
  26. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (Size exclusion chromatography control). (Size exclusion chromatography control)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse IgG-UNLB | \u8ca8\u865f0107-01 \u00a0 \u7522\u54c1\u4ecb\u7d39: \u00a0Goat Anti-Human IgG […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[2258],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19468"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19468"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19468\/revisions"}],"predecessor-version":[{"id":19470,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19468\/revisions\/19470"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19468"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19468"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}