{"id":19424,"date":"2019-04-08T15:34:44","date_gmt":"2019-04-08T07:34:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19424"},"modified":"2022-11-23T14:04:28","modified_gmt":"2022-11-23T06:04:28","slug":"%e4%b9%b3%e9%85%b8%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92ii-lactate-colorimetric-assay-kit-ii-%e8%b2%a8%e8%99%9fk627-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19424","title":{"rendered":"\u4e73\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II Lactate Colorimetric Assay Kit II | \u8ca8\u865fK627-100 (Abcam ab65331)"},"content":{"rendered":"

\u4e73\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II Lactate Colorimetric Assay Kit II\u00a0<\/strong>|\u00a0<\/strong>\u8ca8\u865fK627-100<\/strong>\u00a0 0.02 mM – 10 mM\u4e73\u9178\u00a0\u00a0(Abcam ab65331)<\/span><\/span><\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u4e73\u9178\uff08CH 3 CH\uff08OH\uff09COO-\uff09\u5728\u8a31\u591a\u751f\u7269\u904e\u7a0b\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u7570\u5e38\u9ad8\u6fc3\u5ea6\u7684\u4e73\u9178\u8207\u75be\u75c5\u72c0\u614b\u5982\u7cd6\u5c3f\u75c5\u548c\u4e73\u9178\u9178\u4e2d\u6bd2\u7b49\u6709\u95dc.L\uff08+\uff09 – \u4e73\u9178\u9e7d\u662f\u4eba\u9ad4\u4e2d\u9593\u4ee3\u8b1d\u4e2d\u5f62\u6210\u7684\u4e3b\u8981\u4e73\u9178\u9e7d\u7acb\u9ad4\u7570\u69cb\u9ad4\uff0c\u5b58\u5728\u65bc\u8840\u6db2\u4e2d\u3002 D\uff08 – \uff09 – \u4e73\u9178\u9e7d\u4e5f\u5b58\u5728\uff0c\u4f46\u50c5\u5728L\uff08+\uff09 – \u4e73\u9178\u9e7d\u6fc3\u5ea6\u7684\u7d041-5\uff05\u3002 \u5728\u4e73\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2\u4e2d\uff0c\u4e73\u9178\u9e7d\u88ab\u4e73\u9178\u812b\u6c2b\u9176\u6c27\u5316\uff0c\u7522\u751f\u8207\u63a2\u91dd\u76f8\u4e92\u4f5c\u7528\u7522\u751f\u984f\u8272\uff08\u03bbmax= 450nm\uff09\u7684\u7522\u7269\u3002 \u8a72\u8a66\u5291\u76d2\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\u4e2d\u7684L\uff08+\uff09 – \u4e73\u9178\u9e7d\uff0c\u4f8b\u5982\u8840\u6e05\u6216\u8840\u6f3f\uff0c\u7d30\u80de\uff0c\u57f9\u990a\u7269\u548c\u767c\u9175\u57f9\u990a\u57fa\u3002 \u4e0d\u9700\u8981\u9810\u8655\u7406\u6216\u7d14\u5316\u6a23\u54c1\u3002 \u5b83\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u52300.02 mM – 10 mM\u4e73\u9178\u3002<\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n

\"\"\"\"\"\"<\/p>\n

\"k627\"<\/a><\/p>\n

\"\u5100\u5668\u884c\u92b7<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k627\u03bb= 450 nm\uff09<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK627-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nKit detects 0.02 mM \u2013 10 mM lactate in various samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~40 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains the necessary reagents for accurate measurement of Lactate<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Lactate Assay Buffer
\n\u2022 Lactate Enzyme Mix
\n\u2022 L(+)-Lactate Standard (100 mM)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u5f15\u7528\u6587\u737b\"<\/p>\n

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Zhimin Ou, A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism.\u00a0Cell Rep, March 2019;\u00a0\u00a030865888.<\/a><\/div>\n<\/div>\n
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Mariana Guerra-Maupome, Aerosol vaccination with Bacille Calmette-Guerin induces a trained innate immune phenotype in calves.\u00a0PLoS One, Feb 2019;\u00a030794653.<\/a><\/div>\n<\/div>\n
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Myung Jin Son, A novel and safe small molecule enhances hair follicle regeneration by facilitating metabolic reprogramming.\u00a0Exp Mol Med, Dec 2018;\u00a030523246.<\/a><\/div>\n<\/div>\n
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Qi Cao, Quantification of Hepatic Lipid Using 7.0T Proton Magnetic Resonance Spectroscopy and Computed Tomography in Mild Alcoholic Steatotic Mice.\u00a0J Liver, Dec 2018;\u00a0\u00a030906674.<\/a><\/div>\n<\/div>\n
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Manuela Verri, Plasma energy substrates at two stages of Alzheimer\u2019s disease in humans.\u00a0Int J Immunopathol Pharmacol, Dec 2018;\u00a0PMC6309029.<\/a><\/div>\n<\/div>\n<\/div>\n
Ling, Z. et al., (2017) miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancer,\u00a0Oncology Reports, Jul.2017, 38(3), 1621-1628<\/a><\/div>\n<\/div>\n
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Sun, X. et al., (2017) MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2,\u00a0Bioscience reports, Jun.2017, 37(3), BSR20160404<\/a><\/div>\n<\/div>\n
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Elmorsy, E. et al. (2017) Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics\u00a0Hum Exp Toxicol. 2017 Jun;36(6):616-625.<\/a><\/div>\n<\/div>\n
\n
Sun, Lucia et al. (2017) MicroRNA-143 suppresses oral squamous cell carcinoma (OSCC) cell growth, invasion and glucose metabolism through targeting Hexokinase2,\u00a0Biosci Rep. 2017 Jun 8;37(3).<\/a><\/div>\n<\/div>\n
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Miyata, Saori et al. (2017) Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation,\u00a0Int J Nanomedicine. 2017 Apr 4;12:2703-2716.<\/a><\/div>\n<\/div>\n

\u00a0<\/strong><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e73\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II Lactate Colorimetric Assay Kit II\u00a0|\u00a0\u8ca8\u865fK627- […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2267],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19424"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19424"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19424\/revisions"}],"predecessor-version":[{"id":24344,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19424\/revisions\/24344"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19424"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19424"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19424"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}