{"id":19376,"date":"2019-04-02T16:47:10","date_gmt":"2019-04-02T08:47:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19376"},"modified":"2022-07-20T14:05:36","modified_gmt":"2022-07-20T06:05:36","slug":"%e7%b2%92%e7%b7%9a%e9%ab%94%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-yeast-mitochondria-isolation-kit%e8%b2%a8%e8%99%9fk259-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19376","title":{"rendered":"\u7c92\u7dda\u9ad4\u5206\u96e2\u8a66\u5291\u76d2 | Yeast Mitochondria Isolation Kit\u8ca8\u865fK259-50"},"content":{"rendered":"

\u7c92\u7dda\u9ad4\u5206\u96e2\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K259-50<\/a><\/span><\/p>\n

Yeast Mitochondria Isolation Kit<\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u7c92\u7dda\u9ad4(Mitochondria)\u662f\u7d30\u80de\u7684\u52d5\u529b\u5ba4\uff0c\u56e0\u70ba\u5b83\u5011\u4ee5\u817a\u82f7\u4e09\u78f7\u9178\uff08adenosine tri-phosphate , ATP\uff09\u7684\u5f62\u5f0f\u7522\u751f\u5927\u90e8\u5206\u80fd\u91cf\u4f9b\u61c9\u3002 \u7c92\u7dda\u9ad4 (Mitochondria) \u662f\u96d9\u819c\u7d30\u80de\u5668\uff1a\u5916\u819c(outer membrane)\u548c\u7a31\u70ba\u5d74 (cristae) \u7684\u6298\u758a\u5167\u819c(folded inner membrane)\u3002 \u5206\u96e2\u7684\u7c92\u7dda\u9ad4\u662f\u7814\u7a76\u7c92\u7dda\u9ad4\u547c\u5438(mitochondrial respiration)\u3001\u547c\u5438\u8907\u5408\u7269\u7d44\u88dd(assembly of the respiratory complexes)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u3001mtDNA\u548cmtRNA\u4ee5\u53ca\u86cb\u767d\u8cea\u5206\u6790\u7684\u6709\u7528\u5de5\u5177\u3002 BioVision\u7684Yeast Mitochondria Isolation\u8a66\u5291\u76d2\u53ef\u4ee5\u5feb\u901f\uff0c\u7c21\u4fbf\u5730\u5f9e\u9175\u6bcd\u7d30\u80de\u4e2d\u7d14\u5316\u7c92\u7dda\u9ad4\uff0c\u5229\u7528\u9175\u6bcd\u7d30\u80de\u58c1\u88c2\u89e3\u548c\u5747\u8cea\u5316\u3002 \u8a72\u7c92\u7dda\u9ad4\u5206\u96e2\u8a66\u5291\u76d2\u7d93\u904e\u5df4\u65af\u5fb7\u7562\u8d64\u9175\u6bcd(Pichia pastoris) \u548c\u91c0\u9152\u9175\u6bcd (Saccharomyces cerevisiae) \u7684\u6aa2\u6e2c\uff0c\u53ef\u7528\u65bc\u5f9e\u5176\u4ed6\u9175\u6bcd\u4e2d\u5206\u96e2\u7c92\u7dda\u9ad4\u3002 \u5728\u53ef\u767c\u9175\u57f9\u990a\u57fa(fermentable media)\u4e0b\uff0c\u7522\u91cf\u70ba~150-200\u03bcg\u7c92\u7dda\u9ad4\u548c\u5728\u4e0d\u53ef\u767c\u9175\u57f9\u990a\u57fa\uff08\u4f8b\u5982\u7518\u6cb9\uff09\u4e0b~200-250\u03bcg\u4f86\u81eaOD~20\u57f9\u990a\u7269\u7684\u7c92\u7dda\u9ad4\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK259-50<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIsolation of highly pure intact mitochondria from yeast cells.
\nMitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
\nWestern blot and ELISA<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple & convenient protocol
\n\u2022 The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Buffer A
\n\u2022 Buffer B
\n\u2022 1 M DTT
\n\u2022 Homogenization Buffer
\n\u2022 Lysis Enzyme Mix
\n\u2022 Storage Buffer
\n\u2022 Protease Inhibitor Cocktail<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7c92\u7dda\u9ad4\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865fK259-50 Yeast Mitochondria Isolation Kit \u7522 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2280],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19376"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19376"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19376\/revisions"}],"predecessor-version":[{"id":19377,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19376\/revisions\/19377"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19376"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19376"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19376"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}