{"id":19330,"date":"2019-03-22T11:32:59","date_gmt":"2019-03-22T03:32:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19330"},"modified":"2023-03-17T13:13:42","modified_gmt":"2023-03-17T05:13:42","slug":"%e8%84%82%e8%82%aa%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e5%88%86%e6%9e%90-lipase-activity-colorimetric-assay-kit-ii-%e8%b2%a8%e8%99%9fk723-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19330","title":{"rendered":"\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790 | Lipase Activity Colorimetric Assay Kit II \u8ca8\u865fK723-100 (ABCAM ab102525)"},"content":{"rendered":"

\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K723-100<\/a>\u00a0\u00a0\u00a0(ABCAM ab102525)\u00a0<\/span><\/p>\n

Lipase Activity Colorimetric Assay Kit II<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u8102\u80aa\u9176 (Lipase ) \u662f\u916f\u9176 (\u00a0esterases) \u7684\u4e9e\u985e (\u00a0subclass) \uff0c\u5176\u50ac\u5316\u8102\u8cea\u5e95\u7269\u7684\u916f\u9375\u7684\u6c34\u89e3\u3002 \u5728\u5927\u591a\u6578\u751f\u7269\u9ad4\u4e2d\uff0c\u8102\u80aa\u9176\u5728\u6d88\u5316(\u00a0digestion)\uff0c\u904b\u8f38 (transport) \u548c\u52a0\u5de5\u81b3\u98df\u8102\u8cea\uff08\u4f8b\u5982\u00a0triglycerides\u7518\u6cb9\u4e09\u916f\uff0c\u00a0fats\u8102\u80aa\uff0c\u00a0oils\u6cb9\uff09\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u5728\u4eba\u985e\u4e2d\uff0c\u80f0\u8102\u80aa\u9176 (pancreatic lipase) \u662f\u8ca0\u8cac\u901a\u904e\u5c07\u7518\u6cb9\u4e09\u916f\u8f49\u8b8a\u70ba\u7518\u6cb9\u55ae\u916f\u548c\u6e38\u96e2\u8102\u80aa\u9178\u4f86\u6d88\u5316\u7cfb\u7d71\u4e2d\u7684\u8102\u80aa\u7684\u95dc\u9375\u9176\u3002\u5728\u80f0\u817a\u640d\u50b7\u671f\u9593\uff0c\u8102\u80aa\u9176\u6c34\u5e73\u572824\u301c48\u5c0f\u6642\u5167\u53ef\u4e0a\u53475\u301c10\u500d\u3002 \u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u53ef\u9760\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u5feb\u901f\u5206\u6790\u6a23\u54c1\u4e2d\u7684\u8102\u80aa\u9176\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7279\u5b9a\u5e95\u7269\u4ee5\u7522\u751f\u8207DTNB\u63a2\u91dd\u53cd\u61c9\u4ee5\u7522\u751f\u984f\u8272\uff08\u03bb= 412nm\uff09\u7684\u7522\u7269\u3002 \u8a72\u8a66\u5291\u76d2\u4e5f\u9069\u7528\u65bc\u9ad8\u901a\u91cf\u5206\u6790\u3002<\/p>\n

\"\"<\/p>\n

\u5728K722-100\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7518\u6cb9\u4e09\u9178\u916f\u5e95\u7269\u4ee5\u5f62\u6210\u7518\u6cb9\uff0c\u53ef\u901a\u904e\u76e3\u6e2cOxiRed\u63a2\u91dd\u5438\u5149\u5ea6\uff08\u03bb= 570nm\uff09\u7684\u76f8\u95dc\u8b8a\u5316\u4ee5\u9176\u6cd5\u5b9a\u91cf\u3002 \u5728K723-100\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7279\u5b9a\u7684\u5e95\u7269\u4ee5\u751f\u6210\u7522\u7269\uff0c\u7136\u5f8c\u8207DTNB\u63a2\u91dd\u53cd\u61c9\u751f\u6210\u984f\u8272\uff08\u03bb= 412 nm\uff09\u3002 \u57fa\u672c\u4e0a\uff0c\u5169\u500b\u5957\u4ef6\u7684\u539f\u7406\u662f\u76f8\u540c\u7684\u3002 \u4f46\u662f\uff0c\u6bcf\u7a2e\u8a66\u5291\u76d2\u4f7f\u7528\u7684\u8102\u80aa\u9176\u5e95\u7269\u548c\u6aa2\u6e2c\u63a2\u91dd\u5747\u4e0d\u540c\u3002<\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k723 \u03bb=\u00a0412 nm\uff09<\/span><\/p>\n

\"K723\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK723-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (412 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 60-90 minutes
\n\u2022 Fast and convenient
\n\u2022 Sensitive assays for measuring Lipase activity in various biological samples and the kit is also suitable for high throughput analyses.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Lipase Assay Buffer
\n\u2022 DTNB Probe
\n\u2022 Lipase Substrate
\n\u2022 TNB Standard (5 mM)
\n\u2022 Lipase Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK723-100\u00a0\u00a0\u00a0(ABCAM ab102525)\u00a0 Lipase Ac […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2291],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19330"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19330"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19330\/revisions"}],"predecessor-version":[{"id":40228,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19330\/revisions\/40228"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19330"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19330"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19330"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}