{"id":19326,"date":"2019-03-22T11:17:19","date_gmt":"2019-03-22T03:17:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19326"},"modified":"2024-02-16T16:04:40","modified_gmt":"2024-02-16T08:04:40","slug":"%e8%84%82%e8%82%aa%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e5%88%86%e6%9e%90-lipase-activity-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fk722-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19326","title":{"rendered":"\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790 | Lipase Activity Colorimetric Assay Kit \u8ca8\u865fK722-100 (ABCAM ab102524)"},"content":{"rendered":"

\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K722-100 (ABCAM ab102524)<\/a><\/span><\/p>\n

Lipase Activity Colorimetric Assay Kit<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8102\u80aa\u9176\u5728\u751f\u7269\u9ad4\u4e2d\u7684\u81b3\u98df\u8102\u8cea\uff08\u4f8b\u5982\u8102\u80aa\u548c\u6cb9\uff09\u7684\u6d88\u5316\uff0c\u904b\u8f38\u548c\u52a0\u5de5\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u5728\u4eba\u985e\u4e2d\uff0c\u80f0\u8102\u80aa\u9176\u662f\u901a\u904e\u5c07\u7518\u6cb9\u4e09\u916f\u8f49\u5316\u70ba\u7518\u6cb9\u55ae\u916fmonoglyceride\u548c\u6e38\u96e2\u8102\u80aa\u9178free fatty acid\u4f86\u5206\u89e3\u6d88\u5316\u7cfb\u7d71\u4e2d\u7684\u8102\u80aa\u7684\u95dc\u9375\u9176\u3002 \u80f0\u8102\u80aa\u9176\u76e3\u6e2c\u4e5f\u7528\u65bc\u5e6b\u52a9\u8a3a\u65b7\u514b\u7f85\u6069\u75c5 (Crohn’s disease)\uff0c\u56ca\u6027\u7e96\u7dad\u5316(cystic fibrosis)\u548c\u4e73\u7cdc\u7009(celiac disease)\u3002 \u80f0\u817a\u640d\u50b7\u53ef\u572824\u81f348\u5c0f\u6642\u5167\u8868\u73fe\u51fa\u8840\u6e05\u8102\u80aa\u9176\u6c34\u5e73\u589e\u52a05-10\u500d\u3002 \u5728BioVision\u7684\u8102\u80aa\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7518\u6cb9\u4e09\u916f\u5e95\u7269\u5f62\u6210\u7518\u6cb9glycerol\uff0c\u7518\u6cb9\u901a\u904e\u76e3\u6e2cOxiRed\u63a2\u91dd\u5438\u5149\u5ea6\uff08\u03bb= 570nm\uff09\u7684\u76f8\u95dc\u8b8a\u5316\u9032\u884c\u9176\u4fc3\u5b9a\u91cf\u3002 \u8a72\u6e2c\u5b9a\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\u4e14\u53ef\u9760\uff0c\u4e26\u4e14\u9069\u7528\u65bc\u8102\u80aa\u9176\u7684\u9ad8\u901a\u91cf\u6d3b\u6027\u7be9\u9078\u3002 \u8a72\u8a66\u5291\u76d2\u6aa2\u6e2c\u6bcf\u5b54\u4f4e\u81f30.02 mU\u7684\u8102\u80aa\u9176\u6d3b\u6027lipase activity\u3002\u00a0<\/strong><\/p>\n

<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 (ab102524)<\/strong><\/span><\/div>\n
\n

\u4ee5\u9e7d\u6c34\u3001\u5229\u62c9\u9b6f\u80bd\u3001exendin-4 \u6216\u897f\u4ed6\u5217\u6c40\u6cbb\u7642 75 \u5929\u5f8c\u6e2c\u91cf\u8840\u6f3f\u8102\u80aa\u9176\u6c34\u5e73\uff08\u4f7f\u7528 ab102524\uff09\u3002 ND\uff0c\u6b63\u5e38\u98f2\u98df\uff1b HFD\uff0c\u9ad8\u8102\u98f2\u98df\u3002 p\u22640.05\uff0c*\uff1b p\u22640.01\uff0c**\uff0cn = 3-7 \u96bb\u5c0f\u9f20\u3002<\/span>\u8207\u670d\u7528\u9e7d\u6c34\u7684\u52d5\u7269\u76f8\u6bd4\uff0c\u670d\u7528\u4e09\u7a2e\u85e5\u7269\u7684\u6b63\u5e38\u98f2\u98df\u5c0f\u9f20\u7684\u8840\u6f3f\u8102\u80aa\u9176\u6d3b\u6027\u6c92\u6709\u53ef\u6aa2\u6e2c\u5230\u7684\u5dee\u7570\u3002\u540c\u6a23\uff0c\u8207\u6b63\u5e38\u98f2\u98df<\/span>\u76f8\u6bd4\uff0c<\/span><\/em>\u5728\u9ad8\u8102\u98f2\u98df\u4e2d\u7d66\u4e88\u9e7d\u6c34\u7684\u5c0f\u9f20\u8840\u6f3f\u8102\u80aa\u9176\u6d3b\u6027\u6c92\u6709\u986f\u8457\u8b8a\u5316 \u3002\u6b64\u5916\uff0c\u5229\u62c9\u9b6f\u80bd\u548cExendin-4\u8207\u9ad8\u8102\u98f2\u98df\u5408\u4f75\u7d66\u85e5\u4e5f\u672a\u80fd\u5f71\u97ff\u8840\u6f3f\u8102\u80aa\u9176\u6d3b\u6027\u3002\u8207\u65bd\u7528\u9e7d\u6c34\u7684\u52d5\u7269\u76f8\u6bd4\uff0c\u6211\u5011\u89c0\u5bdf\u5230\u7dad\u6301\u6b63\u5e38\u98f2\u98df\u4e26\u65bd\u7528\u4e09\u7a2e\u85e5\u7269\u4e2d\u7684\u4efb\u4f55\u4e00\u7a2e\u7684\u52d5\u7269\u7684\u8840\u6f3f\u8102\u80aa\u9176\u6d3b\u6027\u6c92\u6709\u53ef\u6aa2\u6e2c\u5230\u7684\u8b8a\u5316\u3002<\/span>\u5716\u7247\u4f86\u81ea Mondragon A \u7b49\u4eba\uff0cPLoS One\u3002 2014\uff1b9(8):e104873\u3002\u57163\u3002\uff1b doi\uff1a10.1371\/journal.pone.0104873\u3002\u6839\u64da\u77e5\u8b58\u5171\u4eab\u6388\u6b0a\u8907\u88fd http:\/\/creativecommons.org\/licenses\/by\/4.0\/<\/em><\/p>\n<\/div>\n

\u5728K722-100\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7518\u6cb9\u4e09\u9178\u916f\u5e95\u7269\u4ee5\u5f62\u6210\u7518\u6cb9\uff0c\u53ef\u901a\u904e\u76e3\u6e2cOxiRed\u63a2\u91dd\u5438\u5149\u5ea6\uff08\u03bb= 570nm\uff09\u7684\u76f8\u95dc\u8b8a\u5316\u4ee5\u9176\u6cd5\u5b9a\u91cf\u3002 \u5728K723-100\u4e2d\uff0c\u8102\u80aa\u9176\u6c34\u89e3\u7279\u5b9a\u7684\u5e95\u7269\u4ee5\u751f\u6210\u7522\u7269\uff0c\u7136\u5f8c\u8207DTNB\u63a2\u91dd\u53cd\u61c9\u751f\u6210\u984f\u8272\uff08\u03bb= 412 nm\uff09\u3002 \u57fa\u672c\u4e0a\uff0c\u5169\u500b\u5957\u4ef6\u7684\u539f\u7406\u662f\u76f8\u540c\u7684\u3002 \u4f46\u662f\uff0c\u6bcf\u7a2e\u8a66\u5291\u76d2\u4f7f\u7528\u7684\u8102\u80aa\u9176\u5e95\u7269\u548c\u6aa2\u6e2c\u63a2\u91dd\u5747\u4e0d\u540c\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK722-100\u00a0(ABCAM ab102524)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis kit detects lipase activity as low as 10mU per well.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 60-90 minutes
\n\u2022 Fast and convenient
\n\u2022 Sensitive assays for measuring Lipase activity in various biological samples.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Lipase Assay Buffer
\n\u2022 OxiRed Probe
\n\u2022 Dimethylsulfoxide (DMSO)
\n\u2022 Enzyme Mix (lyophilized)
\n\u2022 Lipase Substrate
\n\u2022 Glycerol Standard (100mM)
\n\u2022 Lipase Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n
Tilokwattanothai, A. et al., (2017) Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat,\u00a0Chiang Mai Veterinary Journal, 2017, 15(1): 25-35<\/a><\/div>\n<\/div>\n
\n
Anuwat T et al., (2017) Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat,\u00a0Chiang Mai Veterinary Journal, 2017, 15(1): 25-35<\/a><\/div>\n<\/div>\n
\n
Cui K et al., (2017) Ontogenetic development of digestive enzymes in larval and juvenile crimson snapper Lutjanus erythopterus (Bloch 1790),\u00a0Aquaculture Res, 2017, 48(8):4533-4544<\/a><\/div>\n<\/div>\n
\n
Kumar M., Naresh et al. (2016) Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase\u00a0Biosci Rep. 2016 Jul 15;36(4).<\/a><\/div>\n<\/div>\n
\n
Pruszynska-Oszmalek et al., In ovo injection of prebiotics and synbiotics affects the digestive potency of the pancreas in growing chickens.\u00a0Poultry Science,\u00a0<\/strong>Aug 2015; 94: 1909 – 1916.<\/a><\/div>\n<\/div>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8102\u80aa\u9176\u6d3b\u6027\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK722-100 (ABCAM ab102524) Lipase Activ […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,3],"tags":[2292],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19326"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19326"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19326\/revisions"}],"predecessor-version":[{"id":48734,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19326\/revisions\/48734"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19326"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19326"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19326"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}