{"id":19271,"date":"2019-03-18T15:59:59","date_gmt":"2019-03-18T07:59:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19271"},"modified":"2022-07-20T14:17:50","modified_gmt":"2022-07-20T06:17:50","slug":"recombinant-myostatin-mstn%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9frpb653mu01-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19271","title":{"rendered":"Recombinant Myostatin (MSTN)\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fRPB653Mu01"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Active Myostatin (MSTN)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APB653Mu01<\/a><\/span><\/p>\n

\"Active<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Glial Cell Line Derived Neurotrophic Factor (GDNF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\uff0cOrganism Species <\/strong>Mus musculus (Mouse)\u3002Isoelectric Point6.5\uff0c\u7d14\u5ea6> 90%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays\u7b49\u4e0d\u540c\u7814\u7a76\u3002<\/p>\n

\"Active\"APB653Mu01.png\"<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

\u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg\/mL\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\"\"<\/p>\n

MSTN\u8207FSTL3\u7684\u7d50\u5408\u6d3b\u6027\u3002<\/p>\n

\u808c\u8089\u751f\u9577\u6291\u5236\u7d20\uff08MSTN\uff09\u4e5f\u7a31\u70ba\u751f\u9577\u5206\u5316\u56e0\u5b508\uff08GDF-8\uff09\uff0c\u4e00\u7a2e\u7531\u808c\u7d30\u80de\u7522\u751f\u548c\u91cb\u653e\u7684\u86cb\u767d\u8cea\u3002\u5b83\u6291\u5236\u808c\u8089\u751f\u6210\uff0c\u5305\u62ec\u808c\u7d30\u80de\u751f\u9577\u548c\u5206\u5316\u3002\u808c\u8089\u751f\u9577\u6291\u5236\u7d20\u662f\u5206\u6ccc\u7684\u751f\u9577\u5206\u5316\u56e0\u5b50\uff0c\u662fTGF\u03b2\u86cb\u767d\u5bb6\u65cf\u7684\u6210\u54e1\u3002\u6b64\u5916\uff0cFollistatin Like Protein 3\uff08FSTL3\uff09\u5df2\u88ab\u9451\u5b9a\u70baMSTN\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u56e0\u6b64\u9032\u884c\u7d50\u5408ELISA\u6e2c\u5b9a\u4ee5\u6aa2\u6e2c\u91cd\u7d44\u5c0f\u9f20MSTN\u8207\u91cd\u7d44\u5c0f\u9f20FSTL3\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7c21\u8a00\u4e4b\uff0c\u5c07MSTN\u5728\u542b\u67090.01\uff05BSA\uff08pH7.4\uff09\u7684PBS\u4e2d\u9023\u7e8c\u7a00\u91cb\u3002\u7136\u5f8c\u5c07100\u03bcL\u7684\u91cd\u8907\u6a23\u54c1\u8f49\u79fb\u5230FSTL3\u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u572837\u2103\u4e0b\u5b75\u80b22\u5c0f\u6642\u3002\u7528PBST\u6d17\u6ecc\u5b54\u4e26\u8207\u6297MSTN pAb\u4e00\u8d77incubate1\u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u51fa\u4e26\u6d17\u6ecc3\u6b21\u3002\u8207HRP\u6a19\u8a18\u7684\u7b2c\u4e8c\u6297\u9ad4incubate\u5f8c\uff0c\u5438\u51fa\u5b54\u4e26\u6d17\u6ecc3\u6b21\u3002\u52a0\u5165\u5e95\u7269\u6eb6\u6db2\uff0c\u5c07\u5b54\u572837\u2103\u4e0b\u5b75\u80b215-25\u5206\u9418\u3002\u6700\u5f8c\uff0c\u5411\u5b54\u4e2d\u52a0\u516550\u03bcL\u7d42\u6b62\u6eb6\u6db2\uff0c\u7acb\u5373\u8b80\u53d6450nm\u3002 MSTN\u548cFSTL3\u7684\u7d50\u5408\u6d3b\u6027\u5982\u57161\u6240\u793a\uff0c\u8a72\u4f5c\u7528\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u3002<\/p>\n

 <\/p>\n

Active Myostatin (MSTN)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong>\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Mus musculus (Mouse) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
RPB653Mu02<\/td>\nRecombinant Myostatin (MSTN)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
APB653Mu01<\/td>\nActive Myostatin (MSTN)<\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
RPB653Mu01<\/td>\nRecombinant Myostatin (MSTN)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
CPB653Mu21<\/td>\nOVA Conjugated Myostatin (MSTN)<\/a><\/td>\nImmunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAB653Mu08<\/td>\nPolyclonal Antibody to Myostatin (MSTN)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
PAB653Mu02<\/td>\nPolyclonal Antibody to Myostatin (MSTN)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
PAB653Mu01<\/td>\nPolyclonal Antibody to Myostatin (MSTN)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAB653Mu71<\/td>\nBiotin-Linked Polyclonal Antibody to Myostatin (MSTN)<\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
SEB653Mu<\/td>\nELISA Kit for Myostatin (MSTN)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
CEB653Mu<\/td>\nELISA Kit for Myostatin (MSTN)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
KSB653Mu11<\/td>\nELISA Kit DIY Materials for Myostatin (MSTN)<\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u9644Protein marker \u6216 positive control Active Myostatin (MS […]<\/p>\n","protected":false},"author":3,"featured_media":15646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[2300],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19271"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19271"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19271\/revisions"}],"predecessor-version":[{"id":19273,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19271\/revisions\/19273"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19271"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19271"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19271"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}