{"id":19241,"date":"2019-03-15T13:01:17","date_gmt":"2019-03-15T05:01:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19241"},"modified":"2022-07-20T14:18:35","modified_gmt":"2022-07-20T06:18:35","slug":"goat-anti-mouse-igm-fitc%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f1021-02-%e8%a6%8f%e6%a0%bc1-0-mg-1-0-mgml","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19241","title":{"rendered":"Goat Anti-Mouse IgM-FITC\u6297\u9ad4 | \u8ca8\u865f1021-02 \u898f\u683c1.0 mg (1.0 mg\/mL)"},"content":{"rendered":"

Goat Anti-Mouse IgM-FITC\u6297\u9ad4 | \u8ca8\u865f1021-02<\/span> \u00a0<\/strong>\u898f\u683c<\/strong>1.0 mg \u00a0(1.0 mg\/mL)<\/a><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0 Goat Anti-Mouse IgM-FITC\u6297\u9ad4 \uff0c\u5177\u87a2\u5149\u6a19\u8a8c\u3002\u91dd\u5c0dmouse IgM\u91cd\u934a\u548c\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70ba\u5c71\u7f8a\u6297\u8840\u6e05\u3002Cross Adsorption: Mouse IgG and IgA<\/p>\n

Isotype\u70baGoat IgG\u3002Isotype ControlGoat IgG-FITC<\/a> \u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n

\"Goat<\/p>\n

Frozen mouse skeletal muscle tissue was stained with anti-myosin heavy chain and anti-myosin heavy chain type I followed by Goat Anti-Mouse IgG, Human ads-AF555 (SB Cat. No. 1030-32) and Goat Anti-Mouse IgM-FITC (SB Cat. No. 1021-02).Image from Cortez-Toledo O, Schnair C, Sangngern P, Metzger D, Chao LC. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS One. 2017;12(2):e0171268. Figure 3(a)<\/p>\n

\"\"<\/p>\n

FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM-FITC (SB Cat. No. 1021-02).<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with the heavy chain of mouse IgM<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with mouse IgM<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nMouse IgG and IgA; may react with immunoglobulins from other species<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on mouse IgM covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nGoat IgG-FITC<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nFITC (Fluorescein)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA\u00a01-6<\/sup>
\nFLISA
\nFlow Cytometry\u00a04,7-17<\/sup><\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

ELISpot\u00a06<\/sup>
\nImmunohistochemistry-Frozen Sections\u00a018,19<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a020<\/sup>
\nImmunocytochemistry\u00a021,22<\/sup>
\nWestern Blot\u00a023,24<\/sup>
\nStimulation\u00a025,26<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

    \n
  1. Gass JN, Gifford NM, Brewer JW. Activation of an unfolded protein response during differentiation of antibody-secreting B cells. J Biol Chem. 2002;277:49047-54. (ELISA)<\/li>\n
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  26. \u00dcbelhart R, Bach MP, Eschbach C, Wossning T, Reth M, Jumaa H. N-linked glycosylation selectively regulates autonomous precursor BCR function. Nat Immunol. 2010;11:759-65. (Stim)<\/li>\n<\/ol>\n

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    Goat Anti-Mouse IgM-FITC\u6297\u9ad4 | \u8ca8\u865f1021-02 \u00a0\u898f\u683c1.0 mg \u00a0(1.0 […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[2305],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19241"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19241"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19241\/revisions"}],"predecessor-version":[{"id":19244,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19241\/revisions\/19244"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19241"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19241"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}