{"id":19235,"date":"2019-03-14T16:14:32","date_gmt":"2019-03-14T08:14:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19235"},"modified":"2022-07-20T14:18:50","modified_gmt":"2022-07-20T06:18:50","slug":"ezclick-global-rna%e5%90%88%e6%88%90%e6%aa%a2%e6%b8%ac-global-rna-synthesis-assay-kit-facsmicroscopy%e8%b2%a8%e8%99%9fk718-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19235","title":{"rendered":"EZClick\u2122 Global RNA\u5408\u6210\u6aa2\u6e2c| Global RNA Synthesis Assay Kit (FACS\/Microscopy)\u8ca8\u865fK718-100"},"content":{"rendered":"

EZClick\u2122 Global RNA<\/strong>\u5408\u6210\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08FACS \/\u986f\u5fae\u93e1\uff09-\u7d05\u8272\u87a2\u5149 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K718-100<\/a><\/span><\/p>\n

EZClick\u2122 Global RNA Synthesis Assay Kit (FACS\/Microscopy), Red Fluorescence<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>RNA\u5728\u57fa\u56e0\u7684coding\u7de8\u78bc\uff0cdecoding\u89e3\u78bc\u548cregulation\u8abf\u7bc0\u4ee5\u53ca\u6240\u6709\u6d3b\u7d30\u80de\u4e2d\u7684protein expression\u86cb\u767d\u8cea\u8868\u9054\u4e2d\u8d77\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u5728\u5404\u7a2e\u751f\u7406\u689d\u4ef6\u4e0b\u6aa2\u6e2c\u65b0\u5408\u6210\u7684RNA\u6216RNA\u6c34\u5e73\u7684\u8b8a\u5316\uff0c\u6216\u7531\u75be\u75c5\uff0c\u74b0\u5883\u640d\u5bb3\u6216\u85e5\u7269\u6cbb\u7642\u5f15\u8d77\u7684\u80fd\u529b\u662f\u6bd2\u7406\u5b78\u5206\u6790\u7684\u91cd\u8981\u65b9\u9762\u3002\u8a31\u591a\u6297\u764c\u85e5\u7269\u6291\u5236\u8f49\u9304(transcription)\uff0c\u4e26\u4e14\u5927\u591a\u6578\u8f49\u9304\u6291\u5236\u5291\u5177\u6709\u6709\u7528\u7684physiological conditions\u85e5\u7406\u5b78\u6027\u8cea\u3002 Biovision\u7684EZClick\u2122 Global \u00a0RNA\u5408\u6210\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u800c\u5f37\u5927\u7684\u5de5\u5177\uff0c\u7528\u65bc\u5728\u6d3b\u7d30\u80de\u4e2dtemporally\u548cspatially\u6aa2\u6e2cGlobal RNA\u8f49\u9304\u6216\u76f4\u63a5\u6aa2\u6e2cRNA\u6c34\u5e73\u3002\u53ef\u4ee5\u901a\u904e\u7c21\u55ae\u7684\u7a0b\u5e8f\u6aa2\u6e2c\u5f9e\u982d\u5408\u6210\u7684RNA\uff0c\u800c\u7121\u9700\u4f7f\u7528\u653e\u5c04\u6027\u6a19\u8a18(radiolabeling) \u6216\u6297\u9ad4\u3002\u539f\u7406\u70ba\u5c07\u7d30\u80de\u6ef2\u900f\u60275-EU\uff085-Bromo-uridine, \u4e59\u7094\u57fa\u5c3f\u82f7\uff09\u647b\u5165\u65b0\u751fRNA\u4e2d\uff0c\u4f46\u4e0d\u647b\u5165DNA\u4e2d\uff0c\u66ff\u4ee3\u5929\u7136\u5c3f\u82f7\u985e\u4f3c\u7269 (uridine analog) \u3002 5-EU\u53ef\u7528\u4f5cBrU\uff085-\u6eb4 – \u5c3f\u82f7\uff09\u7684\u66ff\u4ee3\u7269\uff0c\u4ee5\u6e2c\u91cf\u589e\u6b96\u7d30\u80de (proliferating cells.) \u4e2d\u5f9e\u982d\u5408\u6210\u7684RNA\u3002\u901a\u904e\u542b\u6709\u758a\u6c2e\u5316\u7269\u7684\u67d3\u6599(azide-containing dye) \u5316\u5b78\u6aa2\u6e2cModified RNA\uff0c\u5176\u80fd\u5920\u8207\u5176\u4ed6\u63a2\u91dd\u9032\u884c\u591a\u91cd\u5206\u6790\uff0c\u6216\u6aa2\u6e2cRNA\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\u4ee5\u7372\u5f97\u66f4\u6df1\u5165\u7684\u751f\u7269\u5b78\u898b\u89e3\u3002\u8a66\u5291\u76d2\u53ef\u901a\u904eFACS\u5206\u6790\u6216\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u6e2c\u3002\u6291\u5236\u5291RNA\u5408\u6210 (\u653e\u7dda\u83cc\u7d20D) \u4f5c\u70ba\u5be6\u9a57\u5c0d\u7167\u7d44\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK718-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Flow Cytometry (Ex\/Em 480\/(530\/590) nm) and Fluorescence Microscopy
\n\u2022 Sample type- Adherent and suspension cells
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- This assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFlow Cytometry (Ex\/Em 480\/(530\/590) nm) and Fluorescence Microscopy<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nSimple, fast, does not require lengthy incubation times<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 EZClick\u2122 Wash Buffer (10X)
\n\u2022 Fixative Solution
\n\u2022 Permeabilization Buffer (10X)
\n\u2022 EZClick\u2122 RNA Label (100X)
\n\u2022 Copper Reagent (100X)
\n\u2022 EZClick\u2122 Fluorescent Azide (100X)
\n\u2022 Reducing Agent (20X)
\n\u2022 DAPI (1000X)
\n\u2022 Actinomycin D (100X)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

EZClick\u2122 Global RNA\u5408\u6210\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08FACS \/\u986f\u5fae\u93e1\uff09-\u7d05\u8272\u87a2\u5149 | \u8ca8\u865fK718-100 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2307],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19235"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19235"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19235\/revisions"}],"predecessor-version":[{"id":19236,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19235\/revisions\/19236"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19235"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19235"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}