{"id":19119,"date":"2019-02-27T10:07:02","date_gmt":"2019-02-27T02:07:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19119"},"modified":"2023-02-03T11:29:50","modified_gmt":"2023-02-03T03:29:50","slug":"endosome%e5%85%a7%e6%b6%b5%e9%ab%94%e5%88%86%e9%9b%a2%e8%90%83%e5%8f%96-endosome-isolation-and-cell-fractionation-kit-invent%e8%b2%a8%e8%99%9fed-028","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=19119","title":{"rendered":"Endosome\u80de\u5167\u9ad4\u5206\u96e2\u8403\u53d6 | Endosome Isolation and Cell Fractionation Kit Invent\u8ca8\u865fED-028"},"content":{"rendered":"

Endosome\u80de\u5167\u9ad4\u5206\u96e2\u548c\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 |<\/strong> Invent\u8ca8\u865fED-028<\/a><\/span><\/p>\n

\"\"Endosome Isolation and Cell Fractionation Kit<\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Early endosomes\u65e9\u671f\u80de\u5167\u9ad4\u4e3b\u8981\u4f86\u81ea\u5f7c\u6b64\u878d\u5408\u7684\u521d\u7d1a\u5167\u541e\u56ca\u6ce1 (primary endocytic vesicles) \uff0c\u70ba\u665a\u671f\u80de\u5167\u9ad4late endosome maturation\u6210\u719f\u4e4b\u8d77\u9ede\u3002\u9664\u4e86\u5728cell physiology\u6b63\u5e38\u7d30\u80de\u751f\u7406\u5b78\u4e2d\u7684\u4f5c\u7528\u5916\uff0cendocytic processes\u5167\u541e\u904e\u7a0b\u5728\u8af8\u5982Alzheimer\u2019s disease\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5\u548c\u907a\u50b3\u6027\u6eb6\u9176\u9ad4\u8caf\u7a4d\u75c5 (inherited lysosomal storage diseases) \u7b49\u8a31\u591a\u75be\u75c5\u4e2d\u626e\u6f14\u95dc\u9375\u4f5c\u7528\u3002\u7528\u65bc\u5206\u96e2endosomes\u7684\u50b3\u7d71\u65b9\u6cd5\u57fa\u65bc\u5bc6\u5ea6\u68af\u5ea6\u8d85\u901f\u96e2\u5fc3 (density gradient ultracentrifugation)\u3002\u8a72\u65b9\u6848\u9700\u8981\u5927\u91cf\u7684\u8d77\u59cb\u6750\u6599\uff0c\u4e26\u4e14tedious\u7e41\u7463\u4e14time-consuming\u8017\u6642\u3002 Minute\u2122 Endosome\u5206\u96e2\u548c\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 (Endosome Isolation and Cell Fractionation Kit, \u8ca8\u865fED028) \u63d0\u4f9b\u57fa\u65bcspin-column-based\u65cb\u8f49\u67f1\u7684\u5d84\u65b0Endosome\u5206\u96e2\u6280\u8853\uff0c\u8a72\u6280\u8853\u5feb\u901f\u7c21\u4fbf\uff0c\u50c5\u9700\u8981\u8f03\u5c11\u6578\u91cf\u7684\u57f9\u990a\u7d30\u80de\u6216mg\u6beb\u514b\u7d44\u7e54\u4e4b\u6aa2\u9ad4\u91cf\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u6c89\u6fb1\u4e26\u986f\u8457\u5206\u96e2\u4f86\u81ea\u57f9\u990a\u7d30\u80de\u6216\u7d44\u7e54\u7684\u65e9\u671f\u7684endosomes\u3002<\/p>\n

\"ed-028\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Buffer A<\/td>\n15 ml<\/td>\n<\/tr>\n
Buffer B<\/td>\n15 ml<\/td>\n<\/tr>\n
Plastic Rods<\/td>\n2 units<\/td>\n<\/tr>\n
Filter Cartridge<\/td>\n20 units<\/td>\n<\/tr>\n
Tissue Dissociation Beads<\/td>\n2.5 grams<\/td>\n<\/tr>\n
Collection Tubes<\/td>\n20 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

ED-028\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

    \n
  1. Xu et al., (2018) Phosphorylation-Mediated IFN-gR2 MembraneTranslocation Is Required to Activate Macrophage Innate Response.\u00a0 Cell 175, 1\u201316.<\/li>\n
  2. Drelich, A., Judy, B., He, X., Chang, Q., Yu, S., Li, X., … & Ksiazek, T. (2018). Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells.\u00a0Viruses<\/em>,\u00a010<\/em>(10), 563.<\/li>\n
  3. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization.\u00a0medRxiv<\/em>.<\/li>\n
  4. Thapa, N., Chen, M., Horn, H. T., Choi, S., Wen, T., & Anderson, R. A. (2020). Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4.\u00a0Nature Cell Biology<\/i>,\u00a022<\/i>(11), 1357-1370.<\/li>\n
  5. Liang, X. H., Nichols, J. G., Hsu, C. W., & Crooke, S. T. (2021). Hsc70 Facilitates Mannose-6-Phosphate Receptor-Mediated Intracellular Trafficking and Enhances Endosomal Release of Phosphorothioate-Modified Antisense Oligonucleotides.\u00a0nucleic acid therapeutics<\/em>.<\/li>\n
  6. Jin, J., Kim, C., Xia, Q., Gould, T. M., Cao, W., Zhang, H., … & Goronzy, J. J. (2021). Activation of mTORC1 at late endosomes misdirects T cell fate decision in older individuals.\u00a0Science Immunology<\/em>,\u00a06<\/em>(60).<\/li>\n
  7. Gong, B., Guo, Y., Ding,\u00a0S.et<\/a>\u00a0al.A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nat Cell Biol (2021).\u00a0https:\/\/doi.org\/10.1038\/s41556-021-00704-y<\/a><\/li>\n<\/ol>\n

    Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

    \u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |PAGE\u86cb\u767d\u8cea\/\u6838\u9178\u63d0\u53d6<\/a><\/span><\/span><\/p>\n

    \u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

    Endosome\u80de\u5167\u9ad4\u5206\u96e2\u548c\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | Invent\u8ca8\u865fED-028 Endosome Isolati […]<\/p>\n","protected":false},"author":3,"featured_media":19120,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[2330],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19119"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19119"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19119\/revisions"}],"predecessor-version":[{"id":37799,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19119\/revisions\/37799"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19120"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19119"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19119"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19119"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}