{"id":18934,"date":"2019-02-19T17:07:28","date_gmt":"2019-02-19T09:07:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18934"},"modified":"2022-07-20T14:35:33","modified_gmt":"2022-07-20T06:35:33","slug":"caspglow%e8%9e%a2%e5%85%89%e7%b4%a0%e6%b4%bb%e6%80%a7caspase-9%e6%9f%93%e8%89%b2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk189","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18934","title":{"rendered":"CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-9\u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK189"},"content":{"rendered":"

CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-9\u67d3\u8272\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K189<\/a><\/span><\/p>\n

CaspGLOW\u2122 Fluorescein Active Caspase-9 Staining Ki-<\/em><\/strong>Detects activated Caspase-9 by FACS, FL or plate reader <\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1aCaspases<\/strong>\u534a\u80f1\u5929\u51ac\u9176\u7684\u6fc0\u6d3b\u5728apoptosis\u7d30\u80de\u51cb\u4ea1\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-9\u67d3\u8272\u8a66\u5291\u76d2\uff0c\u70ba\u6d3b\u7d30\u80de\u4e2d\u6d3b\u5316\u7684caspase-9\u7684\u9748\u654f\u6aa2\u6e2c\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u65b9\u6cd5\u3002 \u8a72\u6e2c\u5b9a\u4f7f\u7528\u8207FITC\u7db4\u5408\u7684\u534a\u80f1\u5929\u51ac\u9176-9\u6291\u5236\u5291LEHD-FMK\uff08FITC-LEHD-FMK\uff09\u4f5c\u70ba\u87a2\u5149\u6a19\u8a18\u7269\u3002 FITC-LEHD-FMK\u662f\u7d30\u80de\u53ef\u6ef2\u900f\u7684\u3001\u7121\u6bd2\u7684\u3001\u4e26\u4e14\u5728\u51cb\u4ea1\u7d30\u80de\u4e2d\u4e0d\u53ef\u9006\u5730\u7d50\u5408\u6d3b\u5316\u7684\u80f1\u5929\u86cb\u767d\u9176-9\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nFluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nSensitive detection of activated caspase-9 in living cells.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes only 1-2 hours
\n\u2022 Fast and convenient
\n\u2022 The FITC label allows for direct detection of the activated caspases in apoptotic cells<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 FITC-LEHD-FMK
\n\u2022 Wash Buffer
\n\u2022 Z-VAD-FMK<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Azad et al., Major Pathways of Polymyxin-Induced Apoptosis in Rat Kidney Proximal Tubular Cells. Antimicrob. Agents Chemother., Apr 2015; 59: 2136 – 2143.<\/p>\n

D\u00e9sio Aur\u00e9lio Farias-de-Oliveira et al., Caspase-8 and caspase-9 mediate thymocyte apoptosis in Trypanosoma cruzi acutely infected mice. J. Leukoc. Biol., Feb 2013; 93: 227 – 234<\/p>\n

Zhnag, X. et. al. Interferon-Description: {gamma} Exacerbates Dry Eye\u2013Induced Apoptosis in Conjunctiva through Dual Apoptotic Pathways. Invest. Ophthalmol. Vis. Sci., Aug 2011; 52: 6279 – 6285.<\/p>\n

Jamadar-Shroff V et al (2009) Clin. Cancer Res.; 15: 1269 – 1276.<\/p>\n

Ito, E. et al. Potential Use of Cetrimonium Bromide as an Apoptosis-Promoting Anticancer Agent for Head and Neck Cancer. Mol. Pharmacol. 2009; 76: 969-983.<\/p>\n

 <\/p>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-9\u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK189 CaspGLOW\u2122 Fluores […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2365],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18934"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18934"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18934\/revisions"}],"predecessor-version":[{"id":18935,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18934\/revisions\/18935"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18934"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18934"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18934"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}