{"id":18853,"date":"2019-02-13T15:37:26","date_gmt":"2019-02-13T07:37:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18853"},"modified":"2023-04-24T11:21:02","modified_gmt":"2023-04-24T03:21:02","slug":"sample-deproteinization%e8%84%ab%e8%9b%8b%e7%99%bd%e6%a8%a3%e5%93%81%e8%a3%bd%e5%82%99%e8%a9%a6%e5%8a%91%e7%9b%92ii-%e8%b2%a8%e8%99%9fk823-200","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18853","title":{"rendered":"Sample deproteinization\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2II | \u8ca8\u865fK823-200 (ABCAM, ab204708)"},"content":{"rendered":"

Sample deproteinization<\/strong>\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2II <\/strong>\u00a0| <\/strong>\u8ca8\u865f<\/strong>K823-200<\/a>\u00a0(ABCAM, ab204708)<\/span><\/p>\n

Deproteinizing Sample Preparation Kit II<\/em><\/strong><\/p>\n

Sample type: Tissue Culture Media, Tissue Extracts, Tissue Lysate, Urine, Whole Blood<\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u751f\u7269\u6d41\u9ad4(biofluids)\u3001\u7d30\u80de(cell)\u3001\u7d30\u83cc(bacteria)\u3001\u9175\u6bcd(yeast)\u548c\u7d44\u7e54\u88c2\u89e3\u7269(tissue lysates)\u7684\u7814\u7a76\u5df2\u7d93\u63d0\u4f9b\u4e86\u6709\u95dc\u5176\u751f\u5316\u6210\u5206\u7684\u91cd\u8981\u4fe1\u606f\u3002\u9019\u4e9b\u751f\u7269\u6a23\u54c1\u5305\u542b\u8c50\u5bcc\uff0c\u8907\u96dc\u7684\u8102\u8cea(lipid)\uff0c\u86cb\u767d\u8cea(proteins)\uff0c\u5c0f\u6709\u6a5f\u5206\u5b50(small organic molecules)\uff0c\u96e2\u5b50(ions)\uff0c\u4ee5\u53ca\u53d6\u6c7a\u65bc\u6a23\u54c1\u7684\u5916\u6e90\u5206\u5b50\uff08\u5373\u85e5\u7269\uff09\u3002\u9084\u53ef\u4ee5\u767c\u73fe\u5e7e\u7a2e\u5c0f\u7684\u6709\u6a5f\u5206\u5b50(small organic molecules)\u6e38\u96e2\u548c\/\u6216\u8207\u5176\u4ed6\u5927\u5206\u5b50\u7d50\u5408\u3002\u9019\u4e9b\u5927\u5206\u5b50\uff08lipoproteins\u8102\u86cb\u767d\uff0cproteins\u86cb\u767d\u8cea\uff0cenzymes\u9176\uff09\u5e72\u64fe(interfere)\u6216\u964d\u89e3(degrade)\u4f4elow molecular-weight metabolites\u5206\u5b50\u91cf\u4ee3\u8b1d\u7269\u3002\u56e0\u6b64\uff0c\u901a\u5e38\u9700\u8981\u6a23\u54c1\u812b\u86cb\u767d(sample deproteinization)\u4f86\u88fd\u5099\u6a23\u54c1\u3002\u5316\u5b78\u812b\u86cb\u767d(Chemical deproteinization)\u65b9\u6cd5\u5982\u4e09\u6c2f\u4e59\u9178\uff08Trichloroacetic acid , TCA\uff09\u662f\u5be6\u73fe\u6a23\u54c1\u812b\u86cb\u767d\u7684\u6700\u5e38\u7528\u65b9\u6cd5\u4e4b\u4e00\u3002BioVision\u7684\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2II\uff0c\u63d0\u4f9b\u7121\u6cd5\u4f7f\u7528\u6709\u6a5f\u6eb6\u5291\u6216\u8d85\u6ffe\u6642\u4e4b\u66ff\u4ee3\u5206\u6790\u65b9\u5f0f\uff0c\u89e3\u6c7a\u4ee3\u8b1d\u7269\u4e0d\u7a69\u5b9a\u6027\u5f71\u97ff\u5c0f\u5206\u5b50\u6fc3\u5ea6\u7684\u6e96\u78ba\u6aa2\u6e2c\u548c\u4f30\u8a08\u3002TCA\u901a\u904e\u5927\u5e45\u964d\u4f4e\u6a23\u54c1pH\u4f86\u6c89\u6fb1\u86cb\u767d\u8cea\u3002\u9664\u53bb\u6c89\u6fb1\u7684\u86cb\u767d\u8cea\u5f8c\uff0c\u7528\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684\u4e2d\u548c\u7de9\u885d\u6db2\u4e2d\u548c\u6a23\u54c1\u7684pH\u3002\u8a72\u8a66\u5291\u76d2\u6613\u65bc\u64cd\u4f5c\uff0c\u4f7f\u7528\u65b9\u4fbf\uff0c\u53ef\u7528\u65bc\u4e26\u884c\u88fd\u5099\u5927\u91cf\u6a23\u54c1\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u88fd\u5099\u7684\u6a23\u54c1\u53ef\u76f4\u63a5\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6e2c\u5b9a\u3002<\/p>\n

\"\"<\/p>\n

TCA\u7528\u91cf\u5c0d\u812b\u86cb\u767d\u7684\u5f71\u97ff\uff1a<\/span><\/strong><\/span><\/div>\n
\n

\u6309\u7167\u8a66\u5291\u76d2\u65b9\u6848\uff0c\u7528\u4e0d\u540c\u91cf\u7684 TCA \u8655\u7406\u6df7\u5408\u4eba\u8840\u6e05\u7684\u7b49\u5206\u8a66\u6a23\uff08\u812b\u96e2\u51dd\u584a\uff0c100 \u03bcL\uff09\u3002\u812b\u86cb\u767d\u524d\u7684\u4f30\u8a08\u86cb\u767d\u6fc3\u5ea6\uff1a7.9 g\/dL\u3002\u4f7f\u7528\u5c11\u65bc 10 \u03bcL TCA\/100 \u03bcL \u4eba\u8840\u6e05\u53ef\u53bb\u9664 99% \u4ee5\u4e0a\u7684\u86cb\u767d\u8cea\u3002\u7d05\u8272\u5be6\u7dda=\u7e3d\u86cb\u767d\uff1b\u85cd\u8272\u865b\u7dda = \u7528 TCA \u8655\u7406\u7684\u6a23\u54c1\uff1b\u7da0\u8272\u5be6\u7dda = \u7528\u65cb\u8f49\u67f1\u8655\u7406\u7684\u6a23\u54c1\u3002<\/p>\n

\u4e73\u9178\u5206\u6790\u6bd4\u8f03\uff1a<\/span><\/strong><\/span><\/p>\n

\u4f7f\u7528 L-\u4e73\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09( ab65331<\/a>\u00a0)\u5206\u6790\u4eba\u8840\u6e05\u6a23\u54c1 (100 \u03bcL) \u7684\u4e73\u9178\u6fc3\u5ea6\u3002\u8207\u7d93 TCA \u8655\u7406\u7684\u6a23\u54c1\uff08\u7d05\u7dda\uff09\u76f8\u6bd4\uff0c\u672a\u7d93\u8655\u7406\u7684\u6a23\u54c1\uff08\u7d2b\u7dda\uff09\u986f\u793a\u51fa\u66f4\u9ad8\u7684\u4fe1\u865f\u3002\u7136\u800c\uff0c\u80cc\u666f\u4fe1\u865f\u5728\u672a\u8655\u7406\uff08\u7da0\u7dda\uff09\u548c\u8655\u7406\uff08\u85cd\u7dda\uff09\u6a23\u672c\u4e2d\u975e\u5e38\u76f8\u4f3c\u3002<\/p>\n<\/div>\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK823-200\u00a0(ABCAM, ab204708)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n200 assays<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nUnique tool to prepare samples for the analysis of a variety of small molecules. Proteins are precipitated without using perchloric acid (PCA)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Fast and convenient
\n\u2022 Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 TCA
\n\u2022 Neutralization Solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Sample deproteinization\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2II \u00a0| \u8ca8\u865fK823-200\u00a0(ABCA […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[2381],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18853"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18853"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18853\/revisions"}],"predecessor-version":[{"id":41579,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18853\/revisions\/41579"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18853"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18853"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18853"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}