{"id":18685,"date":"2019-01-16T17:06:21","date_gmt":"2019-01-16T09:06:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18685"},"modified":"2022-07-20T14:55:55","modified_gmt":"2022-07-20T06:55:55","slug":"%e9%82%84%e5%8e%9f%e5%9e%8b%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bd%ef%bc%88gsh%ef%bc%89%e6%aa%a2%e6%b8%ac-reduced-glutathione-gsh-assay-kit%e8%b2%a8%e8%99%9fk740-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18685","title":{"rendered":"\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c | Reduced Glutathione (GSH) Assay Kit\u8ca8\u865fK740-100"},"content":{"rendered":"

PicoProbe\u2122<\/strong>\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08<\/strong>\u87a2<\/strong>\u5149\uff09 | <\/strong>\u8ca8\u865f<\/strong>K740-100<\/a><\/span><\/p>\n

PicoProbe\u2122 Reduced Glutathione (GSH) Assay Kit (Fluorometric) It specifically detects reduced glutathione. Oxidized GSSG is not detected.<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Glutathione\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u662f\u4e00\u7a2e\u00a0thiol-containing tripeptide\u542b\u5df0\u57fa\u7684\u4e09\u80bd\uff0c\u662f\u751f\u547d\u7cfb\u7d71\u4e2d\u7684\u95dc\u9375\u6297\u6c27\u5316\u5291\u3002 \u7d30\u80de\u5167GSH\u72c0\u614b\u4f3c\u4e4e\u662f\u7d30\u80de\u7e3d\u9ad4\u5065\u5eb7\u72c0\u6cc1\u7684\u6307\u6a19\u3002 BioVision\u7684PicoProbe\u2122\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bcGSH\u548c\u87a2\u5149\u5718\u5b58\u5728\u7684specific enzymatic cycling method\u00a0\u7279\u5b9a\u9176\u5faa\u74b0\u65b9\u6cd5\u3002 \u87a2\u5149\u5718\u7684\u9084\u539f\u7522\u751f\u7a69\u5b9a\u7684\u87a2\u5149\u7522\u7269\uff0c\u5176\u87a2\u5149\u8207\u6a23\u54c1\u4e2dGSH\u7684\u91cf\u6210\u6b63\u6bd4\uff0cfollowed kinetically\u52d5\u529b\u5b78\uff08Ex \/ Em = 535 \/ 587nm\uff09\u3002 \u6211\u5011\u7684\u6aa2\u6e2c\u65b9\u6cd5\u662f\u5e02\u5834\u4e0a\u6700\u5177\u7279\u7570\u6027\u548c\u6700\u654f\u611f\u7684\u3002 \u00a0Oxidized Glutathione\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\u4e0d\u6703\u5e72\u64fe\u6e2c\u5b9a\u3002 \u8a72\u6e2c\u5b9a\u7c21\u55ae\uff0c\u53ef\u91cd\u8907\uff0c\u4e26\u4e14\u53ef\u4ee5\u5728100\u03bcl\u53cd\u61c9\u4e2d\u7279\u7570\u6027\u5730\u6aa2\u6e2c\u4f4e\u81f38pmol \/\u5b54\u7684\u9084\u539f\u5f62\u5f0f\u7684\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u3002<\/p>\n

\"\"\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK740-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (535\/587 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method- Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes only ~1-2 hours
\n\u2022 Fast and convenient
\n\u2022 Specific: does not detect oxidized GSSG
\n\u2022 Sensitive: detects as low as 8 pmol GSH<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 GSH Assay Buffer
\n\u2022 PicoProbeTM (in DMSO)
\n\u2022 Substrate Mix A
\n\u2022 Substrate Mix B
\n\u2022 Enzyme Mix A
\n\u2022 Enzyme Mix B
\n\u2022 Enzyme Mix C
\n\u2022 Sulfosalicylic Acid (SSA, 1 gram)
\n\u2022 GSH Standard<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\u00a0<\/strong><\/p>\n

\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

PicoProbe\u2122\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09 | \u8ca8\u865fK740-100 PicoProbe\u2122 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[122],"tags":[2419],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18685"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18685"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18685\/revisions"}],"predecessor-version":[{"id":18688,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18685\/revisions\/18688"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18685"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18685"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}