{"id":18650,"date":"2019-01-14T17:20:43","date_gmt":"2019-01-14T09:20:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18650"},"modified":"2023-07-14T15:16:57","modified_gmt":"2023-07-14T07:16:57","slug":"%e8%b0%b7%e6%b0%a8%e9%86%af%e8%83%ba%e6%aa%a2%e6%b8%ac-glutamine-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk556-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18650","title":{"rendered":"\u9ea9\u91af\u80fa\u9178 (\u8c37\u6c28\u91af) \u6aa2\u6e2c | Glutamine Colorimetric Assay Kit\u8ca8\u865fK556-100 (ABCAM ab197011)"},"content":{"rendered":"

Glutamine\u00a0\u9ea9\u91af\u80fa\u9178 (\u8c37\u6c28\u91af\u80fa) \u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K556-100 (ABCAM ab197011)<\/a><\/span><\/p>\n

Glutamine Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8c37\u6c28\u91af\u80fa\uff08Glutamine,\u00a0<\/em><\/strong>Gln\uff09\u662f\u542b\u6709\u4e0d\u5e36\u96fb\u7684\u91af\u80fa\u4f5c\u70ba\u5074\u93c8\u7684\u6700\u8c50\u5bcc\u7684\u6c28\u57fa\u9178\u4e4b\u4e00\u3002 \u5b83\u901a\u904e\u8c37\u6c28\u9178(glutamate )\u548c\u6c28(\u00a0ammonia)\u7684\u7e2e\u5408\u5408\u6210\u3002 Gln\u88ab\u6b78\u985e\u70ba\u975e\u5fc5\u9700\u6c28\u57fa\u9178(non-essential amino acid)\uff0c\u7136\u800c\uff0cGln\u5728\u5e7e\u7a2e\u751f\u7269\u904e\u7a0b\u4e2d\u662f\u91cd\u8981\u7684\uff0c\u4f8b\u5982\u86cb\u767d\u8cea\u5408\u6210(Protein synthesis)\uff0c\u54fa\u4e73\u52d5\u7269\u814e\u81df\u4e2d \u9178\u5e73\u8861(acid-balance )\u7684\u8abf\u7bc0\u548c\u7d30\u80de\u751f\u9577\u3002 \u5b83\u69cb\u6210\u4e86\u7d30\u80de\u6838\u82f7\u9178(nucleotides )\u548c\u5df1\u7cd6\u80fa(hexosamines)\u5408\u6210\u7684\u4e3b\u8981\u6c2e\u6e90\u3002 \u5bcc\u542b\u8c37\u6c28\u91af\u80fa\u7684\u98f2\u98df\u6709\u76ca\u60a3\u6709\u514b\u7f85\u6069\u75c5(Crohn\u2019s disease)\uff0c\u56b4\u91cd\u71d2\u50b7(severe burns)\uff0c HIV\/AIDS\u827e\u6ecb\u75c5\u6bd2\/\u827e\u6ecb\u75c5\u548c\u764c\u75c7(cancer)\u7684\u60a3\u8005\u3002 BioVision\u7684\u8c37\u6c28\u91af\u80fa\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7c21\u55ae\u9748\u654f\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u6aa2\u6e2c\u5404\u7a2e\u6db2\u9ad4\u548c\u7d44\u7e54\u4e2d\u751f\u7269\u5b78\u76f8\u95dc\u7684Glutamine\u6fc3\u5ea6\u3002 \u8a72\u6e2c\u5b9a\u57fa\u65bc\u8c37\u6c28\u91af\u80fa\u6c34\u89e3\u70baGlutamine\uff0c\u7522\u751f\u7a69\u5b9a\u7684\u4fe1\u865f\uff0c\u5176\u8207\u6a23\u54c1\u4e2d\u7684Glutamine\u7684\u91cf\u6210\u6b63\u6bd4\u3002 \u8a72\u6e2c\u5b9a\u53ef\u4ee5\u5728\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u5c11\u81f325\u03bcM\u7684Glutamine\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK556-100 (ABCAM ab197011)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of Glutamine in various biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Convenient & Rapid Protocol
\n\u2022 This kit can detect as little as 25 \u00b5M of Glutamine in a variety of biological samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Hydrolysis Buffer
\n\u2022 Development Buffer
\n\u2022 Hydrolysis Enzyme Mix (Lyophilized)
\n\u2022 Development Enzyme Mix (Lyophilized)
\n\u2022 Developer (Lyophilized)
\n\u2022 Gln Standard (Lyophilized)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Leandro R. Soria, Hepatic glutamine synthetase augmentation enhances ammonia detoxification.,\u00a0J Inherit Metab Dis, Nov. 2019, 30724386<\/a><\/p>\n

Mokgadi Violet Gwangwa, Effects of glutamine deprivation on oxidative stress and cell survival in breast cell lines.\u00a0Biol Res, Mar 2019; 30917872.<\/a><\/p>\n

Mus, F. et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of\u00a0glnE\u00a0deletion in\u00a0Azotobacter vinelandii,\u00a0Appl. Environ. Microbiol, Oct.2017, online<\/a><\/p>\n

Mus F et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of glnE deletion in Azotobacter vinelandii,\u00a0Appl. Environ. Microbiol., 2017, April 21: epub<\/a><\/p>\n

Lim et al., Targeting Mitochondrial Oxidative Metabolism in Melanoma Causes Metabolic Compensation through Glucose and Glutamine Utilization.\u00a0Cancer Res.,\u00a0Jul 2014; 74: 3535 – 3545.<\/a><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Glutamine\u00a0\u9ea9\u91af\u80fa\u9178 (\u8c37\u6c28\u91af\u80fa) \u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK556-100 (ABCAM ab197 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5380,3],"tags":[2426],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18650"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18650"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18650\/revisions"}],"predecessor-version":[{"id":44027,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18650\/revisions\/44027"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18650"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18650"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18650"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}