{"id":18608,"date":"2019-01-10T11:51:40","date_gmt":"2019-01-10T03:51:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18608"},"modified":"2023-12-01T16:46:41","modified_gmt":"2023-12-01T08:46:41","slug":"%e6%b8%b8%e9%9b%a2%e7%94%98%e6%b2%b9%e6%aa%a2%e6%b8%ac-free-glycerol-colorimetricfluorometric-assay-kit%e8%b2%a8%e8%99%9fk630-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18608","title":{"rendered":"\u6e38\u96e2\u7518\u6cb9\u6aa2\u6e2c | Free Glycerol Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK630-100 (Abcam ab65337)"},"content":{"rendered":"

(\u4e0d\u540c\u6a23\u54c1\u4f86\u6e90)\u00a0\u6e38\u96e2\u7518\u6cb9\u6bd4\u8272\/<\/strong>\u87a2<\/strong>\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K630-100<\/a>\u00a0\u00a0(Abcam ab65337)\u00a0<\/span><\/p>\n

Free Glycerol Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\u7518\u6cb9\u5ee3\u6cdb\u7528\u65bc\u98df\u54c1\uff0c\u98f2\u6599\uff0c\u6eb6\u5291\uff0c\u85e5\u7269\u548c\u5316\u599d\u54c1\u7b49\u3002\u4eba\u5011\u5c0d\u7528\u65bc\u7814\u7a76\u548c\u958b\u767c\u7684\u7518\u6cb9\u7684\u5b9a\u91cf\u5177\u6709\u5ee3\u6cdb\u7684\u8208\u8da3\u3002 BioVision\u7684\u7518\u6cb9\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u9748\u654f\uff0c\u7c21\u4fbf\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u6e2c\u91cf\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u6e38\u96e2\u7518\u6cb9\u6fc3\u5ea6\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u7518\u6cb9\u88ab\u9176\u4fc3\u6c27\u5316\u4ee5\u7522\u751f\u8207\u63a2\u91dd\u53cd\u61c9\u4ee5\u7522\u751f\u984f\u8272\uff08\u03bb= 570nm\uff09\u548c\u87a2\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u7684\u7522\u7269\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d50pmol-10nmol\uff08\u62161~10000\u03bcM\u7bc4\u570d\uff09\u7684\u7518\u6cb9\u3002<\/p>\n

\"\"<\/p>\n

\"K630\"<\/a><\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k630\u00a0 \u03bb= 570 nm\uff09<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK630-100\u00a0(Abcam ab65337)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit can detect 50 pmol-10 nmol (or 1~10000 \u00b5M range) of glycerol in various samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 40 minutes
\n\u2022 Fast and convenient
\n\u2022 The kit provides an accurate assay for measuring free glycerol in various samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Glycerol Assay Buffer
\n\u2022 Glycerol Probe (in DMSO, anhydrous)
\n\u2022 Glycerol Enzyme Mix (lyophilized)
\n\u2022 Glycerol Standard (100 mM)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
<\/div>\n
\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"<\/div>\n
Iso, T. et al., (2017) Serum levels of fatty acid binding protein 4 and fat metabolic markers in relation to catecholamines following exercise,\u00a0Clinical Biochemistry, Jun.2017, https:\/\/doiorg\/101016\/jclinbiochem201705021<\/a><\/div>\n<\/div>\n
\n
Tsao et al, Interleukin-4 regulates lipid metabolism by inhibiting adipogenesis and promoting lipolysis.\u00a0J. Lipid Res., Mar 2014; 55: 385 – 397.<\/a><\/div>\n<\/div>\n
\n
MacDonald et al., IL-6 and epinephrine have divergent fiber type effects on intramuscular lipolysis.\u00a0J Appl Physiol, Nov 2013; 115: 1457 – 1463.<\/a><\/div>\n<\/div>\n
\n
Tetsuro Izumi et al., Activin Receptor-Like Kinase 7 Suppresses Lipolysis to Accumulate Fat in Obesity Through Downregulation of Peroxisome Proliferator\u2013Activated Receptor\u00a0 and C\/EBP\u03b1.\u00a0Diabetes, Jan 2013; 62: 115 – 123.<\/a><\/div>\n<\/div>\n
\n
\u00d6zlem G\u00f6gebakan et al., Glucose-Dependent Insulinotropic Polypeptide Reduces Fat-Specific Expression and Activity of 11\u03b2-Hydroxysteroid Dehydrogenase Type 1 and Inhibits Release of Free Fatty Acids,\u00a0Diabetes, Feb 2012; 61: 292 – 300<\/a>.<\/div>\n<\/div>\n

 <\/p>\n

\u00a0\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"smart\"<\/a>\"Spectrumlab<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

(\u4e0d\u540c\u6a23\u54c1\u4f86\u6e90)\u00a0\u6e38\u96e2\u7518\u6cb9\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK630-100\u00a0\u00a0(Abcam ab65337)\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,466,3],"tags":[2432],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18608"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18608"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18608\/revisions"}],"predecessor-version":[{"id":41598,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18608\/revisions\/41598"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18608"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18608"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18608"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}