{"id":18533,"date":"2019-01-04T13:22:43","date_gmt":"2019-01-04T05:22:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18533"},"modified":"2022-07-20T15:04:34","modified_gmt":"2022-07-20T07:04:34","slug":"asfv%e9%9d%9e%e6%b4%b2%e8%b1%ac%e7%98%9f%e6%aa%a2%e6%b8%ac%e5%a5%97%e7%b5%84-african-swine-fever-virus-elisa-%e8%b2%a8%e8%99%9fasfs-5p","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18533","title":{"rendered":"ASFV\u975e\u6d32\u8c6c\u761f\u6aa2\u6e2c\u5957\u7d44 |African swine fever virus ELISA \u8ca8\u865fASFS-5P"},"content":{"rendered":"

ASFV\u975e\u6d32\u8c6c\u761f\u6aa2\u6e2c\u5957\u7d44 |\u8ca8\u865fASFS-5P<\/a><\/span><\/p>\n

Multi-antigen indirect ELISA kit for the detection of antibodies against P32, P62 and P72 of the ASF virus<\/p>\n

\u975e\u6d32\u8c6c\u761f\u75c5\u6bd2(African swine fever virus\uff1bASFV)\u3002\u5c6c\u65bcAsfaviridae\u75c5\u6bd2\u79d1\uff0cAsfivirus\u5c6c\u3002\u75c5\u6bd2\u4e4b\u6297\u9178\u9e7c\u80fd\u529b\u5f37\uff0c\u53ef\u5b58\u6d3b\u65bc\u9178\u9e7c\u503c4\u81f313\u3002\u8c6c\u662f\u552f\u4e00\u6703\u88ab ASFV \u611f\u67d3\u7684\u7d93\u6fdf\u52d5\u7269\u3002\u5be6\u9a57\u5ba4\u8a3a\u65b7\u65b9\u6cd5\u6709\uff1b\u75c5\u6bd2\u6297\u539f\u5075\u6e2c(ASFV antigen detection)\u3001\u75c5\u6bd2\u5206\u96e2(Virus Isolation)\u3001\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u53cd\u61c9(ELISA)\u3001\u75c5\u6bd2\u6838\u9178\u6aa2\u6e2c(DNA detection)\u3001PCR or qPCR\u5b9a\u5e8f(Sequencing)\u3001\u75c5\u6bd2\u6297\u9ad4\u6aa2\u6e2c(ASFV antibody detection)\u3002\u66f4\u591a\u8cc7\u6599\u4f86\u6e90\u5bb6\u755c\u885b\u751f\u8a66\u9a57\u6240<\/a><\/span><\/p>\n

\"\"<\/p>\n

African swine fever virus (ASFV) causes an economically important disease of pigs. It is a large complex DNA virus and is the only member of the\u00a0Asfarviridae<\/em>\u00a0family, genus\u00a0Asfivirus.<\/em>\u00a0ASFV has several layers that surround\u00a0a dense core containing its DNA genome. The image shows virus particles at different stages of assembly. (reference from\u00a0https:\/\/www.pirbright.ac.uk\/asfv)<\/p>\n

IDScreen\u00ae<\/u>\u975e\u6d32\u8c6c\u761f<\/u>\u6aa2\u6e2c\u5957\u7d44<\/u> (<\/u>\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>| Indirect ELISA\u8ca8\u865fASFS-5P\uff0c\u591a\u6297\u539f\u9593\u63a5ELISA\u8a66\u5291\u76d2\uff0c\u7528\u65bc\u6aa2\u6e2c\u8c6c\u8840\u6e05\uff0c\u8840\u6f3f\u6216\u8840\u6db2\u6ffe\u7d19\u6a23\u54c1\u4e2dASF\u75c5\u6bd2P32\uff0cP62\u548cP72\u7684\u6297\u9ad4\u3002\u57fa\u65bc3\u7a2e\u91cd\u7d44\u6297\u539f\u7684\u552f\u4e00\u5546\u696dELISA\uff1aP32\uff0cP62\u548cP72\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\uff0c\u660e\u78ba\u5206\u96e2\u967d\u6027\u548c\u9670\u6027\u7d50\u679c\u3002\u53ef\u4ee5\u572896\u5b54\u6df1\u5b54\u677f\u4e2d\u6e2c\u8a66\u8840\u6db2\u6ffe\u7d19\u6a23\u54c1\uff0c\u4f7f\u6a23\u54c1\u8655\u7406\u66f4\u5feb\u4e26\u4e14\u66f4\u4e0d\u5bb9\u6613\u6df7\u6dc6\u3002<\/p>\n\n\n\n\n\n\n\n
Method<\/strong><\/td>\nIndirect ELISA<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nSwine (domestic pigs and wild boar)<\/td>\n<\/tr>\n
Specimens<\/strong><\/td>\nSerum, plasma, meat juice or blood filter paper
\nsamples<\/td>\n<\/tr>\n
Coated antigen<\/strong><\/td>\nMix of P32, P62 and P72 recombinant proteins<\/td>\n<\/tr>\n
Conjugate<\/strong><\/td>\nAnti-multi-species-HRP concentrated conjugate (10X)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100<\/a>\u00a0|\u00a0Motic AE31E\u53ef\u5347\u7d1a\u5012\u7acb\u5f0f\u986f\u5fae\u93e1<\/a>\u00a0 |\u00a0PD-30 \u624b\u6301\u5f0f ATP \u5fae\u751f\u7269\u51b7\u5149\u5100<\/a><\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

ASFV\u975e\u6d32\u8c6c\u761f\u6aa2\u6e2c\u5957\u7d44 |\u8ca8\u865fASFS-5P Multi-antigen indirect ELISA ki […]<\/p>\n","protected":false},"author":3,"featured_media":8631,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[2443],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18533"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18533"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18533\/revisions"}],"predecessor-version":[{"id":18534,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18533\/revisions\/18534"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8631"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18533"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18533"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}