{"id":18438,"date":"2018-12-20T17:51:10","date_gmt":"2018-12-20T09:51:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18438"},"modified":"2022-07-20T15:05:52","modified_gmt":"2022-07-20T07:05:52","slug":"anti-ley-br96-human-igg1-kappa-absoluteantibody%e8%b2%a8%e8%99%9fab00492-10-0","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18438","title":{"rendered":"Anti-LeY [BR96], Human IgG1, Kappa | absoluteantibody\u8ca8\u865fAb00492-10.0"},"content":{"rendered":"<p><strong>Fc Silent\u2122 Antibodies: Turning down the background noise<\/strong> <strong>\u53ea\u8981<\/strong><strong>\u8a0a<\/strong><strong>\u865f\u4e0d\u8981\u96dc<\/strong><strong>\u8a0a (Engineered Fc Domain)<\/strong><\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=18438\"><strong>Anti-LeY [BR96], Human IgG1, Kappa | <\/strong><strong>\u8ca8\u865f<\/strong><strong>Ab00492-10.0<\/strong><\/a><\/span><strong><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=18438\">\u00a0<\/a><\/span>\u00a0 1 mg\/ml.<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/absoluteantibody.com\/wp-content\/uploads\/2018\/09\/Logo-crop_image-angled-365x372.png\" alt=\"\" width=\"197\" height=\"200\" \/><\/p>\n<p>LeY(Lewis Y.) tetrasaccharide\u56db\u7cd6\u662f\u9078\u64c7\u6027\u764c\u7d30\u80de\u8868\u9762\u8868\u9054\u7684carbohydrate antigen\u78b3\u6c34\u5316\u5408\u7269\u6297\u539f\uff0c\u5305\u62eccolon\u7d50\u8178\u764c\uff0cbreast\u4e73\u817a\u764c\uff0covary\u5375\u5de2\u764c\u548clung\u80ba\u764c\u3002\u7814\u7a76\u8868\u660e\uff0c\u55ae\u514b\u9686\u6297\u9ad4\u5c0d\u6297\u539f\u967d\u6027\u816b\u7624\u7d30\u80de\u5177\u6709toxic effects\u6bd2\u6027\u4f5c\u7528\uff0c\u5728\u6fc3\u5ea6\u4f4e\u81f30.1\u03bcg\/ ml\u6642\u9032\u884cADCC\uff08\u6297\u9ad4\u4f9d\u8cf4\u7684\u7d30\u80de\u4ecb\u5c0e\u7684\u7d30\u80de\u6bd2\u6027\u4f5c\u7528\uff0cantibody-dependent cell-mediated cytotoxicity\uff0c<strong>ADCC<\/strong>\uff09\u3002\u6297\u9ad4B396\u5728\u67d0\u4e9b\u764c\u7d30\u80de\u7cfb\u4e2d\u4ee50.1-5.0\u03bcg\/ ml\u7684\u6fc3\u5ea6\u9032\u884c\u88dc\u9ad4\u4f9d\u8cf4\u6027\u7d30\u80de\u6bd2\u6027\u3002\u55ae\u514b\u9686\u6297\u9ad4\u9084\u5177\u6709\u88ab\u6297\u539f\u967d\u6027\u816b\u7624\u7d30\u80de\u5167\u5316\u7684\u80fd\u529b\uff0c\u56e0\u6b64\u53ef\u7528\u65bc\u5c07\u6297\u764c\u85e5\u7269\u905e\u9001\u81f3\u7279\u5b9a\u816b\u7624\u7d30\u80de\u3002Lewis Y.\u6297\u9ad4\u5176\u514d\u75ab\u539f\u7531\u4eba\u4e73\u817a\u764c\u8f49\u79fb\u5efa\u7acb\u7684H3396\u57f9\u990a\u7cfb\uff0c\u7531Protein A affinity\u65b9\u5f0f\u7d14\u5316\u65bcPBS with 0.02% Proclin 300.\u7de9\u885dBuffer\u3002\u7279\u7570\u6027\uff1a\u6297\u9ad4\u4ee50.1\u03bcM\u7684Ka\u7d50\u5408Le\uff08Y\uff09\u3002\u8caf\u5b58\u689d\u4ef6tore at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/absoluteantibody.com\/wp-content\/uploads\/2018\/08\/IMG00384Ab00492-1.1IFfull.png\" alt=\"Binding Curve\" width=\"496\" height=\"500\" \/><\/p>\n<p><b>Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 (Ab00492)\u00a0<\/b>Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix\u2122 coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (Ab00492-1.1) at 10 \u00b5g\/ml for 1h followed by Alexa Fluor\u00ae\u00a0488 secondary antibody (2 \u00b5g\/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00492-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor\u00ae\u00a0488 secondary antibody.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fc Silent\u2122 Antibodies: Turning down the background nois [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6981,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2454,2455,2456],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18438"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18438"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18438\/revisions"}],"predecessor-version":[{"id":18441,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18438\/revisions\/18441"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6981"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18438"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18438"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18438"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}