{"id":18385,"date":"2018-12-17T14:35:55","date_gmt":"2018-12-17T06:35:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18385"},"modified":"2025-08-07T16:26:32","modified_gmt":"2025-08-07T08:26:32","slug":"asparagine%e5%a4%a9%e5%86%ac%e9%85%b0%e8%83%ba%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk736-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18385","title":{"rendered":"Asparagine\u5929\u51ac\u91af\u80fa\u9178\u6aa2\u6e2c | ABCAM\u8ca8\u865fK736-100 (Asparagine Assay Kit (Fluorometric))"},"content":{"rendered":"

Asparagine\u5929\u51ac\u91af\u80fa\u9178<\/em><\/strong><\/a><\/span>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/em><\/strong> | <\/strong>\u8ca8\u865f<\/strong>K736-100 (ABCAM ab273333)<\/a><\/span><\/p>\n

First assay in the market that measures Asparagine in biological Samples<\/em><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Asparagine\u5929\u51ac\u91af\u80fa\u9178\u662f\u86cb\u767d\u8cea\u751f\u7269\u5408\u6210\u4e2d\uff0c\u6240\u6709\u751f\u7269\u9ad4\u4f7f\u7528\u768420\u7a2e\u5929\u7136\u5b58\u5728\u7684\u6c28\u57fa\u9178\u4e4b\u4e00\u3002\u5728\u4eba\u985e\u4e2d\uff0c\u5b83\u88ab\u8a8d\u70ba\u662fnon-essential amino acid\u975e\u5fc5\u9700\u6c28\u57fa\u9178\uff0c\u56e0\u70ba\u5b83\u53ef\u4ee5\u5728\u9ad4\u5167\u7531aspartate\u5929\u51ac\u6c28\u9178\u548cglutamine\u8c37\u6c28\u9170\u80fa\u5408\u6210\u3002\u9664\u4e86\u4f5c\u70baprotein building block\u7684\u4f5c\u7528\u5916\uff0casparagine\u5929\u51ac\u91af\u80fa\u9178\u5c0d\u65bc\u7522\u524d\u548c\u7522\u5f8c\u795e\u7d93\u5143\u767c\u80b2\u81f3\u95dc\u91cd\u8981\u3002\u5148\u5929\u6027\u7f3a\u4e4f\u7684\u5929\u51ac\u91af\u80fa\u9178\u5408\u6210\u9176\uff08EC 6.3.5.4\uff09\u5c0e\u7dfbmicrocephaly\u5c0f\u982d\u7578\u5f62\u3001intractable seizures\u9811\u56fa\u6027\u7672\u7647\u767c\u4f5c\u548c\u56b4\u91cd\u7684neurodevelopmental disabilities\u795e\u7d93\u767c\u80b2\u969c\u7919\u3002\u76f8\u53cd\uff0c\u5929\u51ac\u91af\u80fa\u9178\u6c34\u5e73\u7684\u6d88\u8017\u88ab\u7528\u4f5c\u67d0\u4e9b\u764c\u75c7\u7684\u6cbb\u7642\u7b56\u7565\uff0c\u7279\u5225\u662facute lymphoblastic leukemia\u6025\u6027\u6dcb\u5df4\u7d30\u80de\u767d\u8840\u75c5\uff08ALL\uff09\u548c\u5176\u4ed6\u9020\u8840\u7cfb\u7d71\u764c\u75c7\u3002\u6dcb\u5df4\u6bcd\u7d30\u80de\u4e0d\u80fd\u5f9e\u982d\u5408\u6210\u5929\u51ac\u9170\u80fa\uff0c\u56e0\u6b64\u4f9d\u8cf4\u65bc\u5916\u6e90\u6027\u4f86\u6e90\u3002\u7528\u7d30\u83cc\u9176\u5929\u51ac\u9170\u80fa\u9176\uff08EC 3.5.1.1\uff09\u8655\u7406\u4f7f\u60e1\u6027\u7d30\u80de\u55aa\u5931\u5feb\u901f\u751f\u9577\u3002 BioVision\u7684\u5929\u51ac\u91af\u80fa\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u5c0d\u751f\u7269\u9ad4\u6db2\u548c\u57f9\u990a\u7d30\u80de\u4e2d\u7684L-\u5929\u51ac\u91af\u80fa\u9178\u6c34\u5e73\u9032\u884c\u9ad8\u9748\u654f\u5ea6\u5b9a\u91cf\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cL-\u5929\u51ac\u91af\u80fa\u9178\u88ab\u6c34\u89e3\u4ee5\u7522\u751f\u5929\u51ac\u6c28\u9178\uff0c\u5176\u88ab\u8f49\u5316\u70ba\u4e19\u916e\u9178\u4e26\u96a8\u5f8c\u901a\u904e\u4e00\u7cfb\u5217\u5076\u806f\u7684\u9176\u4fc3\u53cd\u61c9\u8207\u87a2\u5149\u63a2\u91dd\u53cd\u61c9\u4ee5\u5f62\u6210\u7a69\u5b9a\u7684\u87a2\u5149\u5718\uff08Ex \/ Em = 538 \/ 587nm\uff09\u3002\u8a72\u6e2c\u5b9a\u4e0d\u53d7\u5176\u4ed6\u5e38\u898b\u6c28\u57fa\u9178\u7684\u751f\u7406\u6fc3\u5ea6\u7684\u5f71\u97ff\uff0c\u4e26\u4e14\u53ef\u4ee5\u6aa2\u6e2c\u6a23\u54c1\u4e2d\u4f4e\u81f35\u03bcM\u7684Asparagine\u5929\u51ac\u91af\u80fa\u9178\u6c34\u5e73\u3002<\/p>\n

Protocol Summary<\/strong><\/span><\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK736-100 (ab273333)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (Ex\/Em= 535\/587 nm)
\n\u2022 Species reactivity- Eurkayotes and Prokaryotes<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em= 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes and Prokaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of Asparagine in biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 60 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains the necessary reagents for accurate measurement of Asparagine<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Asparagine Assay Buffer
\n\u2022 Probe Solution
\n\u2022 Asparagine Enzyme Mix
\n\u2022 Conversion Enzyme Mix
\n\u2022 Developer Enzyme Mix
\n\u2022 Sample Cleanup Mix
\n\u2022 L-Asparagine Standard<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Materials Supplied<\/span><\/strong><\/span><\/p>\n

<\/p>\n

\u8a66\u5291\u88fd\u5099 (Reagent Preparation)<\/span><\/span><\/pre>\n
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\u6a23\u54c1\u88fd\u5099 (Sample Preparation)<\/span><\/span><\/p>\n
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\u5178\u578b\u6a19\u6e96\u66f2\u7dda\uff0d\u6578\u64da\u50c5\u4f9b\u53c3\u8003\u4e4b\u7528\u3002<\/span><\/span><\/p>\n
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 <\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"
Asparagine\u5929\u51ac\u91af\u80fa\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK736-100 (ABCAM ab273333) Firs […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[2466],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18385"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18385"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18385\/revisions"}],"predecessor-version":[{"id":52191,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18385\/revisions\/52191"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18385"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18385"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18385"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}