{"id":18304,"date":"2018-12-09T11:51:14","date_gmt":"2018-12-09T03:51:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18304"},"modified":"2024-06-18T18:35:54","modified_gmt":"2024-06-18T10:35:54","slug":"%e4%ba%9e%e7%a1%9d%e9%85%b8%e9%b9%bd%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-griess-reagent-nitrite-assay-kit%e8%b2%a8%e8%99%9fk544-200","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18304","title":{"rendered":"\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2 (Griess Reagent) | Nitrite Assay Kit\u8ca8\u865fk544-200 (abcam ab234044)"},"content":{"rendered":"

\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2 (Griess Reagent) | <\/strong>\u8ca8\u865f<\/strong>k544-200\u00a0<\/a><\/span> (abcam ab234044)\u00a0 \u4e9e\u785d\u9178\u9e7d\uff08Nitrite; NO2 – \uff09\u7d2f\u7a4d\u91cf\u6e2c\u5b9a\u5957\u7d44-Griess Reagent<\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

 <\/p>\n

\u5728\u5e7e\u4e4e\u6bcf\u500b\u751f\u7269\u9ad4\u4e2d\u90fd\u767c\u73fe\u4e86Nitrogen-based ions\u6c2e\u57fa\u96e2\u5b50\uff0cNitrogen-based ions\u4e9e\u785d\u9178\u9e7d\u548cnitrate\u785d\u9178\u9e7d\u3002\u6b64\u5916\uff0c\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\u767c\u73fe\u5167\u6e90\u4e9e\u785d\u9178\u9e7d\u6c34\u5e73\uff0c\u4e5f\u53ef\u5f9edietary sources\u81b3\u98df\u4f86\u6e90\u7372\u5f97\u3002\u5728\u4eba\u985e\u4e2d\uff0cnitrit\u4e9e\u785d\u9178\u9e7d (nitrite) \u9032\u4e00\u6b65\u4ee3\u8b1d\u70ba\u4e00\u6c27\u5316\u6c2e(Nitric Oxide)\u548c\u5176\u4ed6\u6d3b\u6027\u6c2e\u7269\u8cea\uff08reactive nitrogen species, nitrogen oxides\u6c2e\u6c27\u5316\u7269\uff09\u3002\u785d\u9178\u9e7d – \u4e9e\u785d\u9178\u9e7d –\u00a0 NO\u751f\u5316\u9014\u5f91\u56e0\u5176\u53c3\u8207\u7d30\u80de\u4fe1\u865f\u50b3\u5c0e(cell signaling)\uff0c\u7f3a\u6c27\u4f9d\u8cf4\u6027\u53cd\u61c9(hypoxia-dependent response )\u548c\u8840\u6d41\u8abf\u7bc0\u800c\u773e\u6240\u5468\u77e5\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\u7dda\u7c92\u9ad4\u4e2d\u4e9e\u785d\u9178\u9e7d\u9084\u539f\u70ba\u6c2e\u6c27(Nitrogen Oxygen )\u3002\u5177\u9ad4\u800c\u8a00\uff0c\u808c\u7d05\u86cb\u767d(myoglobin)\u548c\u9ec3\u560c\u5464\u6c27\u5316\u9084\u539f\u9176(xanthine oxidoreductase)\u53ef\u5728\u4f4e\u6c27\u689d\u4ef6\u4e0b\u7522\u751fNO\uff0c\u5f9e\u800c\u5c0e\u81f4\u7dda\u7c92\u9ad4\u547c\u5438\u3002BioVision\u7684\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2\u4f7f\u7528Griess Reagent\uff0c\u9019\u662f\u4e00\u7a2e\u7528\u65bc\u4f30\u7b97\u4e9e\u785d\u9178\u9e7d\u7684\u5e38\u7528\u6e2c\u91cf\u65b9\u5f0f\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u4f7f\u7528Griess\u8a66\u5291I\u5c07\u4e9e\u785d\u9178\u9e7d\u9084\u539f\u70ba\u6c2e\u6c27\u5316\u7269\u3002\u7136\u5f8c\uff0c\u6c2e\u6c27\u5316\u7269\u8207Griess Reagent II\u53cd\u61c9\u5f62\u6210\u7a69\u5b9a\u7684\u7522\u7269\uff0c\u5176\u53ef\u901a\u904e\u5176\u5728OD 540nm\u8655\u7684\u5438\u5149\u5ea6\u4f86\u6aa2\u6e2c\u3002\u4e00\u500b\u6b65\u9a5f\u5feb\u901f\u7c21\u55ae\u5206\u6790\uff0c\u6aa2\u6e2c\u4f4e\u81f31 nmol \/\u5b54\u7684\u4e9e\u785d\u9178\u9e7d\u6c34\u5e73\u3002<\/p>\n

\"K544\"<\/a><\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k544 \u03bb= 540 nm\uff09<\/span><\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n\n\n\n\n\n\n\n\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (540 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- BioVision\u2019s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method- Absorbance (540 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nNitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple two-step procedure; takes only ~1-1.5 hours
\n\u2022 Fast and convenient
\n\u2022 BioVision\u2019s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 Griess Reagent R1
\n\u2022 Griess Reagent R2
\n\u2022 Nitrite Standard<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2 (Griess Reagent) | \u8ca8\u865fk544-200\u00a0 (abcam ab23404 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[183],"tags":[2478],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18304"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18304"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18304\/revisions"}],"predecessor-version":[{"id":18307,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18304\/revisions\/18307"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18304"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}