{"id":18299,"date":"2018-12-07T16:50:04","date_gmt":"2018-12-07T08:50:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18299"},"modified":"2022-07-20T15:15:14","modified_gmt":"2022-07-20T07:15:14","slug":"cd44-set-sirnashrnarnai-lentivector-human-abm-%e8%b2%a8%e8%99%9fi000054","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18299","title":{"rendered":"CD44-set siRNA\/shRNA\/RNAi Lentivector (Human) | ABM \u8ca8\u865fi000054"},"content":{"rendered":"

CD44-set siRNA\/shRNA\/RNAi Lentivector (Human)\u8f09\u9ad4 | \u8ca8\u865fi000054<\/a><\/span> 4 x 500 ng<\/p>\n

NM_000610<\/a> <\/span>\u8f09\u9ad4\u8cc7\u6599piLenti-siRNA-GFP<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>CD44-set siRNA\/shRNA\/RNAi Lentivector\u8868\u73fe\u8f09\u9ad4\uff1b \u7d93\u7531\u7d14\u5316\u5f8c\u4e4bplasmid DNA\u53ef\u76f4\u63a5\u975e\u75c5\u6bd2\u8cea\u7c92\u8f49\u67d3\u7acb\u5373\u8868\u9054; \u6216\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\uff0c\u7528\u65bc\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u8868\u9054\u3002<\/p>\n

\"\"\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Application<\/strong><\/td>\n\n

\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n

\u00b7\u00a0 Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n

Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_000610<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npiLenti-siRNA-GFP<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\n\n

CD44-V1-697: 5′–ACACAACCTCTGGTCCTATAAGGACACCC–3′<\/p>\n

CD44-V6-2159: 5′–GGTGTAACACCTACACCATTATCTTGGAA–3′<\/p>\n

CD44-V6-1160: 5′–TGAGGGATATCGCCAAACA–3′<\/p>\n

CD44-Scramble: 5′–AAGAACCCAATTAAGCGCAAG–3′<\/td>\n<\/tr>\n

Negative Control<\/strong><\/td>\nFor negative scramble control with neomycin use Catalog\u00a0LV015-N<\/a><\/td>\n<\/tr>\n
Sequencing Primers<\/strong><\/td>\nTACGTCCAAGGTCGGGCAGGAAGA<\/td>\n<\/tr>\n
Bacterial Selection<\/strong><\/td>\nKanamycin<\/td>\n<\/tr>\n
Mammalian Selection<\/strong><\/td>\nPuromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n-20\u00b0C or below<\/td>\n<\/tr>\n
Shelf Life<\/strong><\/td>\n1 year (when at -20\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped at ambient temperature<\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nRestriction Enzyme Digest and Sequencing<\/td>\n<\/tr>\n
Caution<\/strong><\/td>\nThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CD44-set siRNA\/shRNA\/RNAi Lentivector (Human)\u8f09\u9ad4 | \u8ca8\u865fi00 […]<\/p>\n","protected":false},"author":3,"featured_media":2030,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[2479],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18299"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18299"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18299\/revisions"}],"predecessor-version":[{"id":18303,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18299\/revisions\/18303"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2030"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18299"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18299"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18299"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}