{"id":18291,"date":"2018-12-06T10:28:50","date_gmt":"2018-12-06T02:28:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18291"},"modified":"2022-07-20T15:15:32","modified_gmt":"2022-07-20T07:15:32","slug":"%e5%94%be%e6%b6%b2%e9%85%b8%ef%bc%88nana%ef%bc%89%e6%af%94%e8%89%b2%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk566-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18291","title":{"rendered":"\u553e\u6db2\u9178\u6aa2\u6e2c | Sialic Acid (NANA) Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK566-100"},"content":{"rendered":"

\u553e\u6db2\u9178\uff08NANA\uff09\u6bd4\u8272\/<\/em><\/strong>\u87a2<\/em><\/strong>\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2<\/em><\/strong> | <\/strong>\u8ca8\u865f<\/strong>K566-100<\/a><\/span><\/p>\n

Sialic Acid (NANA) Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u6240\u6709\u810a\u690e\u52d5\u7269\u7d30\u80de\u7684\u8868\u9762\u90fd\u88dd\u98fe\u8457\u5bc6\u96c6\u800c\u8907\u96dc\u7684\u7cd6\u93c8 (sugar chains)\uff0c\u9019\u4e9b\u7cd6\u93c8\u4e3b\u8981\u9644\u8457\u5728\u86cb\u767d\u8cea\u548c\u8102\u8cea\u4e0a\u3002\u5927\u591a\u6578\u53ef\u6eb6\u6027\u5206\u6ccc\u86cb\u767d (\u00a0secreted proteins) \u4e5f\u7528\u9019\u7a2e\u805a\u91a3\u985e (glycans)\u4f3c\u5730decorated\u3002\u553e\u6db2\u9178 (Sialic acid, NANA)\u662f\u795e\u7d93\u6c28\u9178(neuraminic acid)\u7684N-\u6216O-\u53d6\u4ee3\u884d\u751f\u7269 (\u00a0N- or O-substituted derivatives) \u7684\u901a\u7a31\uff0c\u795e\u7d93\u6c28\u9178 (neuraminic acid) \u662f\u5177\u6709nine-carbon backbone 9-\u78b3\u9aa8\u67b6\u7684\u55ae\u91a3 (monosaccharide ) \u3002 \u5b83\u4e5f\u662f\u8a72\u7d44\u4e2d\u6700\u5e38\u898b\u6210\u54e1\u7684\u540d\u7a31\uff0c N-\u4e59\u9170\u795e\u7d93\u6c28\u9178 (N-acetylneuraminic acid)\u3002 \u553e\u6db2\u9178 (Sialic acids )\u5ee3\u6cdb\u5206\u4f48\u5728\u52d5\u7269\u7d44\u7e54\u4e2d\uff0c\u4e26\u4e14\u5728\u8f03\u5c0f\u5206\u4f48\u5728\u5f9e\u690d\u7269(plants) \u3001\u771f\u83cc (fungi) \u5230\u9175\u6bcd (yeasts) \u548c\u7d30\u83cc (bacteria) \u7684\u5176\u4ed6\u7269\u7a2e\u4e2d\uff0c\u4e3b\u8981\u5728\u7cd6\u86cb\u767d(glycoproteins)\u548c\u795e\u7d93\u7bc0\u82f7\u8102(gangliosides) \u4e2d\u3002 \u9451\u65bc\u5b83\u5011\u7684\u4f4d\u7f6e\u548c\u666e\u904d\u5206\u4f48\uff0c\u553e\u6db2\u9178\u53ef\u4ee5\u4ecb\u5c0e\u6216\u8abf\u7bc0\u5404\u7a2e\u751f\u7406\u548c\u75c5\u7406\u904e\u7a0b\u3002\u00a0\u6700\u8fd1\u5df2\u7d93\u8868\u660e\uff0c\u553e\u6db2\u9178(sialic acid )\u6c34\u5e73\u53ef\u80fd\u8207\u767c\u80b2(developmental) \u548c\u75c5\u7406\u968e\u6bb5(pathological stages)\u6709\u95dc\u3002 BioVision\u7684\u553e\u6db2\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 (Sialic Acid Assay Kit, K566) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u6e38\u96e2\u553e\u6db2\u9178 (free Sialic Acid)\u3002 \u8a72\u8a66\u5291\u76d2\u5229\u7528\u9176\u5076\u806f\u53cd\u61c9\uff0c\u5176\u4e2d\u6e38\u96e2\u7684\u553e\u6db2\u9178\u88ab\u6c27\u5316\uff0c\u5c0e\u81f4Oxi-Red\u63a2\u91dd\u7522\u751f\u87a2\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u548c\u5438\u5149\u5ea6\uff08O.D. = 570nm\uff09\u3002 \u8a72\u8a66\u5291\u76d2\u6e2c\u91cf\u553e\u6db2\u9178\u7684\u7dda\u6027\u7bc4\u570d\u70ba0.1\u81f310 nmol\uff0c\u6aa2\u6e2c\u9748\u654f\u5ea6\u70ba~1\u03bcM\u6fc3\u5ea6\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK566-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 \u00b5M concentration.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~40 minutes
\n\u2022 Fast and convenient
\n\u2022 The kit utilizes an enzyme coupled reaction in which free sialic acid is oxidized resulting in development of the Oxi-Red probe to give color<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Sialic Acid Assay Buffer
\n\u2022 Sialic Acid Probe in DMSO
\n\u2022 Sialic Acid Converting Enzyme
\n\u2022 Sialic Acid Development Mix
\n\u2022 Sialic Acid Standard (10 \u00b5mol)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

6<\/strong>\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/strong><\/a>\u00a0|\u00a0<\/strong>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u553e\u6db2\u9178\uff08NANA\uff09\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK566-100 Sialic Acid (NANA) Col […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2481],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18291"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18291"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18291\/revisions"}],"predecessor-version":[{"id":20607,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18291\/revisions\/20607"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18291"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18291"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18291"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}