{"id":18234,"date":"2018-11-19T11:31:27","date_gmt":"2018-11-19T03:31:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18234"},"modified":"2024-12-04T14:34:47","modified_gmt":"2024-12-04T06:34:47","slug":"caspglow%e8%9e%a2%e5%85%89%e7%b4%a0%e6%b4%bb%e6%80%a7caspase-3%e6%9f%93%e8%89%b2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk183-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18234","title":{"rendered":"CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-3\u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK183-100"},"content":{"rendered":"

CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-3\u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK183-100<\/a><\/span><\/p>\n

CaspGLOW\u2122 Fluorescein Active Caspase-3 Staining Kit-Detects activated Caspase-3 by FACS, FL or plate reader<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1aCaspases<\/strong>\u534a\u80f1\u5929\u51ac\u9176\u7684\u6fc0\u6d3b\u5728\u7d30\u80de\u51cb\u4ea1\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-3\u67d3\u8272\u8a66\u5291\u76d2\uff0c\u70ba\u6d3b\u7d30\u80de\u4e2d\u6d3b\u5316\u7684caspase-3\u7684\u9748\u654f\u6aa2\u6e2c\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u65b9\u6cd5\uff0c\u53ef\u61c9\u7528\u65bcFACS\u3001FL\u3001\u6216\u8005Plate reader\u3002 \u8a72\u6e2c\u5b9a\u4f7f\u7528\u8207FITC\uff08FITC-DEVD-FMK\uff09\u7db4\u5408\u7684\u534a\u80f1\u5929\u51ac\u9176-3\u6291\u5236\u5291DEVD-FMK\u4f5c\u70ba\u6a19\u8a18\u7269\u3002 FITC-DEVD-FMK\u662f\u7d30\u80de\u53ef\u6ef2\u900f\u7684\uff0c\u7121\u6bd2\u7684\uff0c\u4e26\u4e14\u5728\u51cb\u4ea1\u7d30\u80de\u4e2d\u4e0d\u53ef\u9006\u5730\u7d50\u5408\u6d3b\u5316\u7684\u80f1\u5929\u86cb\u767d\u9176-3 caspase-3\u3002<\/p>\n\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nFluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nSensitive detection of activated caspase-3 in living cells.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes only 1-2 hours\u2022 Fast and convenient\u2022 The FITC label allows for direct detection of the activated caspases in apoptotic cells<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 FITC-DEVD-FMK
\n\u2022 Wash Buffer
\n\u2022 Z-VAD-FMK<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

HSU, F. T et al., (2017) Regorafenib Induces Apoptosis and Inhibits Metastatic Potential of Human Bladder Carcinoma Cells, Anticancer Research, Jul.2017, 37(9), 4919-4926<\/p>\n

Chiang IT et al., (2017) Hyperforin Inhibits Cell Growth by Inducing Intrinsic and Extrinsic Apoptotic Pathways in Hepatocellular Carcinoma Cells, Anticancer Res, 2017, 37(1)161-167<\/p>\n

Hung et al., Homocysteine facilitates LOX-1 activation and endothelial death through the PKC\u03b2 and SIRT1\/HSF1 mechanism: relevance to human hyperhomocysteinaemia.\u00a0 Clin. Sci., Sep 2015; 129: 477 – 487.<\/p>\n

Azad et al., Major Pathways of Polymyxin-Induced Apoptosis in Rat Kidney Proximal Tubular Cells. Antimicrob. Agents Chemother., Apr 2015; 59: 2136 – 2143.<\/p>\n

Wei et al., Involvement of general control nonderepressible kinase 2 in cancer cell apoptosis by posttranslational mechanisms. Mol. Biol. Cell, Mar 2015; 26: 1044 – 1057.<\/p>\n

Tiwari et al., MicroRNA-125a Reduces Proliferation and Invasion of Oral Squamous Cell Carcinoma Cells by Targeting Estrogen-related Receptor \u03b1: IMPLICATIONS FOR CANCER THERAPEUTICS. J. Biol. Chem., Nov 2014; 289: 32276 – 32290.<\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

CaspGLOW\u2122\u87a2\u5149\u7d20\u6d3b\u6027Caspase-3\u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK183-100 CaspGLOW\u2122 Flu […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[128],"tags":[2492],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18234"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18234"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18234\/revisions"}],"predecessor-version":[{"id":50466,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18234\/revisions\/50466"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18234"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18234"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18234"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}