{"id":18216,"date":"2018-11-16T15:48:48","date_gmt":"2018-11-16T07:48:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18216"},"modified":"2025-08-21T10:03:58","modified_gmt":"2025-08-21T02:03:58","slug":"hydroxyproline%e7%be%a5%e8%84%af%e6%b0%a8%e9%85%b8-%e8%b2%a8%e8%99%9fk226-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18216","title":{"rendered":"Hydroxyproline\u7fa5\u812f\u6c28\u9178 | \u8ca8\u865fK226-100 (ABCAM ab222941)"},"content":{"rendered":"

Hydroxyproline\u7fa5\u812f\u6c28\u9178 | <\/strong>\u8ca8\u865f<\/strong>K226-100 (ABCAM ab222941)<\/a><\/span><\/p>\n

This kit does not use PCA to detect and quantify Hydroxyproline<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Hydroxyproline\u7fa5\u812f\u6c28\u9178\uff08\uff082S\uff0c4R\uff09-4-\u7fa5\u57fa\u812f\u6c28\u9178\uff0cHyp\uff09\u662f\u7531\u812f\u6c28\u9170-4-\u7fa5\u5316\u9176\u5f62\u6210\u7684nonproteinogenic amino acid\u975e\u86cb\u767d\u539f\u6c28\u57fa\u9178\uff0c\u86cb\u767d\u8cea\u5408\u6210\u5f8c\u50ac\u5316\u591a\u80bd\u812f\u6c28\u9178\u6b98\u57fa\u7684\u5f8c\u8f49\u8b6f\u4fee\u98fe\u3002\u5728\u52d5\u7269\u4e2d\uff0chydroxyproline\u7fa5\u812f\u6c28\u9178\u5e7e\u4e4e\u5168\u90e8\u5b58\u5728\u65bc\u81a0\u539f\u86cb\u767d\u4e2d\uff0c\u5176\u542b\u6709\u7d0412-14\uff05\u7684\u7fa5\u812f\u6c28\u9178\uff08\u539f\u7e96\u7dad\u86cb\u767d\u5f48\u6027\u86cb\u767d\u9084\u542b\u6709\u5c11\u91cf\u7684\u7fa5\u812f\u6c28\u9178\uff09\u3002\u56e0\u6b64\uff0c\u7d44\u7e54\u6c34\u89e3\u7522\u7269\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u70ba\u76f4\u63a5\u6e2c\u91cf\u81a0\u539f\u86cb\u767d\u91cf\u542b\u91cf\u3002\u81a0\u539f\u86cb\u767d\u662f\u54fa\u4e73\u52d5\u7269\u4e2d\u6700\u8c50\u5bcc\u7684\u86cb\u767d\u8cea\uff0c\u8a31\u591a\u75be\u75c5\u88ab\u8a8d\u70ba\u6703\u5f71\u97ff\u81a0\u539f\u86cb\u767d\u7684\u8f49\u63dbcollagen turnover\uff0c\u5305\u62ectumor invasion\u816b\u7624\u4fb5\u8972\u548cmetastasis\u8f49\u79fb\uff0crheumatoid arthritis\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e\uff0cCardiopulmonary fibrosis\u5fc3\u80ba\u7e96\u7dad\u5316\u548cmuscular dystrophy\u808c\u71df\u990a\u4e0d\u826f\u3002\u6e2c\u91cfHydroxyproline\u7684\u50b3\u7d71\u65b9\u6cd5\u57fa\u65bcHydroxyproline\u6c27\u5316\u6210pyrrole intermediate\u5421\u54af\u4e2d\u9593\u9ad4\uff0c\u7136\u5f8c\u8207\u6eb6\u89e3\u5728\u6fc3\u9ad8\u6c2f\u9178\uff08HClO4\uff09\u4e2d\u7684Ehrlich\u8a66\u5291\u53cd\u61c9\u3002Perchloric acid\u9ad8\u6c2f\u9178\u662f\u4e00\u7a2e\u6709\u6bd2\u7269\u8cea\uff0c\u5177\u6709\u6bd2\u6027\u548c\u9ad8\u53cd\u61c9\u6027\uff0c\u9700\u8981\u7279\u6b8a\u8655\u7406\u548c\u5ee2\u7269\u8655\u7406\u5354\u8b70\u3002 BioVision\u7684Hydroxyproline Assay Kit II\uff08\u4e0d\u542b\u9ad8\u6c2f\u9178\u9e7d\uff09\u4f7f\u7528\u5c08\u6709\u7684\u9178\u6027\u986f\u5f71\u5291\u6eb6\u6db2\uff0c\u7121\u9700\u4f7f\u7528\u6709\u5bb3\u7684\u9ad8\u6c2f\u9178\u9e7d\u5373\u53ef\u6e96\u78ba\u6e2c\u91cf\u7d44\u7e54\u548c\u86cb\u767d\u8cea\/\u80bd\u9176\u6c34\u89e3\u7522\u7269\u4e2d\u7684Hydroxyprolin\u3002\u9019\u662f\u4e00\u7a2e\u5feb\u901f\u65b9\u4fbf\u7684\u65b9\u6848\uff0c\u7522\u751f\u984f\u8272\u9bae\u8c54\u7684\u9aee\u8272\u5718\uff0c\u5728560 nm\u8655\u5177\u6709\u5438\u6536\u5cf0\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u4ee5\u572896\u5b54\u683c\u5f0f\u4e2d\u6aa2\u6e2c\u6bcf\u5b54\u6700\u5c110.05\u03bcg\u7fa5\u812f\u6c28\u9178\uff0c\u4e26\u7522\u751f\u8207\u50b3\u7d71\u7684\u9ad8\u6c2f\u9178\u57fa\u65b9\u6cd5\u5e7e\u4e4e\u76f8\u540c\u7684\u6bd4\u8272\u7d50\u679c\u3002<\/p>\n

<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK226-100 (ABCAM ab222941)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 Reactions<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (560 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- The assay is useful over the range of 0.1-1 \u03bcg.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD = 560 nm).<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nEstimation of hydroxyproline concentration in various biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~60 minutes
\n\u2022 Convenient and easy to use
\n\u2022 Faster than Perchlorate-Based Assay
\n\u2022 PCA-free<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Oxidation Buffer
\n\u2022 Chloramine T Concentrate
\n\u2022 Developer Solution
\n\u2022 DMAB Concentrate (in DMSO)
\n\u2022 Hydroxyproline Standard (1 mg\/ml)
\n\u2022 Microplate Sealing Film<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Wang, M., Et al., (2017) , Increasing Dietary Medium-Chain Fatty Acid Ratio Mitigates High-fat Diet-Induced Non-Alcoholic Steatohepatitis by Regulating Autophagy,\u00a0Sci. Rep., 2017, 7: 13999<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Hydroxyproline\u7fa5\u812f\u6c28\u9178 | \u8ca8\u865fK226-100 (ABCAM ab222941) This k […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2493],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18216"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18216"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18216\/revisions"}],"predecessor-version":[{"id":52401,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18216\/revisions\/52401"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18216"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18216"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18216"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}