{"id":18073,"date":"2018-10-25T11:36:41","date_gmt":"2018-10-25T03:36:41","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18073"},"modified":"2022-07-20T15:20:11","modified_gmt":"2022-07-20T07:20:11","slug":"%e7%92%b0%e6%b0%a7%e5%90%88%e9%85%b6%ef%bc%88cox%ef%bc%89%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e8%9e%a2%e5%85%89%ef%bc%89-%e8%b2%a8%e8%99%9fk549-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=18073","title":{"rendered":"\u74b0\u6c27\u5408\u9176\uff08COX\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09 | \u8ca8\u865fK549-100"},"content":{"rendered":"

\u74b0\u6c27\u5408\u9176\uff08COX\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>K549-100<\/a><\/span><\/p>\n

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1aCyclooxygenase (COX)<\/strong>\u74b0\u6c27\u5408\u9176\uff08COX\uff09\uff0c\u4e5f\u7a31\u70ba\u524d\u5217\u817a\u7d20 – \u5167\u904e\u6c27\u5316\u7269\u5408\u9176\uff08prostaglandin-endoperoxide synthase, PTGS\uff0cEC 1.14.99.1\uff09\uff0c\u662f\u4e00\u7a2e\u8ca0\u8cac\u5f62\u6210\u7a31\u70baprostanoids\u524d\u5217\u817a\u7d20\u7684\u91cd\u8981\u751f\u7269\u4ecb\u8cea\u7684\u9176\uff0c\u5305\u62ec\u524d\u5217\u817a\u7d20prostaglandins\uff0cprostaglandins\u524d\u5217\u74b0\u7d20\u548cthromboxane\u8840\u6813\u7d20\u3002 COX\u662f\u5f9earachidonic acid\u82b1\u751f\u56db\u70ef\u9178\u5230\u524d\u5217\u817a\u7d20\u7684\u751f\u7269\u5408\u6210\u9014\u5f91\u4e2d\u7684\u4e2d\u5fc3\u9176\u3002\u6709\u5169\u7a2e\u5df2\u77e5\u7684\u540c\u5de5\u9176\uff1aCOX-1\u548cCOX-2\u3002 COX-1\u5728\u8a31\u591a\u7d44\u7e54\u4e2d\u7d44\u6210\u578b\u8868\u9054\uff0c\u4e26\u4e14\u662fgastric mucosa\u80c3\u7c98\u819c\u548ckidney\u814e\u81df\u4e2d\u7684\u4e3b\u8981\u5f62\u5f0f\u3002\u5728\u5927\u591a\u6578\u7d30\u80de\u4e2d\uff0cCOX-2\u5728\u6b63\u5e38\u689d\u4ef6\u4e0b\u4e0d\u8868\u9054\uff0c\u4f46\u5728inflammation\u708e\u75c7\u671f\u9593\u89c0\u5bdf\u5230\u5347\u9ad8\u7684\u6c34\u5e73\u3002\u975e\u753e\u9ad4\u6297\u708e\u85e5\uff08NSAID\uff09\u5c0dCOX\u7684\u85e5\u7406\u5b78\u6291\u5236\u53ef\u4ee5\u7de9\u89e3\u708e\u75c7\u548c\u75bc\u75db\u7684\u75c7\u72c0\u3002 BioVision\u7684COX\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u9ad8\u901a\u91cf\u7684\u9069\u61c9\u6027\u65b9\u6cd5\uff0c\u7528\u65bc\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\u6216\u7d14\u5316\/\u7c97\u9176\u88fd\u5291\u4e2dCOX\u7684\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ecCOX-1\u548cCOX-2\u7279\u7570\u6027\u6291\u5236\u5291\uff0c\u4ee5\u5340\u5206COX-1\u548cCOX-2\u4ee5\u53ca\u53ef\u80fd\u5b58\u5728\u65bc\u6a23\u54c1\u4e2d\u7684\u5176\u4ed6\u904e\u6c27\u5316\u7269\u9176\u7684\u6d3b\u6027\u3002\u6aa2\u51fa\u9650\uff1a6\u03bcU\/ mg\u3002\u6b64\u7522\u54c1\u53ef\u6e2c\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff0c\u6216\u662f\u7d93\u7531\u548c\u7d14\u5316\/\u7c97\u9176\u88fd\u5291\u4e2d\u4e4bCOX\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u6a23\u54c1\u985e\u578b\uff1a<\/strong>
\n\u7c98\u9644\u548c\u61f8\u6d6e\u7d30\u80de (Adherent and suspension cells) \u3001\u52d5\u7269\u7d44\u7e54 (Animal tissues )\uff0c\u5982\u5927\u9f20\u809d\u81df (rat liver)\u3001\u7d14\u5316\u9176 (Purified enzyme)<\/p>\n

\u7528\u6236\u81ea\u884c\u9700\u63d0\u4f9b\u7684\u8a66\u5291\u548c\u8a2d\u5099<\/strong>
\n—96\u5b54\u767d\u8272\u4e0d\u900f\u660e\u677f\uff0c\u5e73\u5e95
\n—\u76e4\u5f0f\u5206\u5149\u5149\u5ea6\u8a08\uff08\u6216\u87a2\u5149\u677f\u8b80\u6578\u5668\uff09
\n—Multi-channel pipette\uff08\u53ef\u8abf\u81f310\u03bcl\uff09
\n—DMSO\uff0c1X PBS\uff0cLysis Buffer \u88c2\u89e3\u7de9\u885d\u6db2\uff08\u4f8b\u59821\u00d7\u542b1\uff05NP40\u7684PBS\uff09\uff0cProtease inhibitor Cocktail\uff08Cat\uff03K272\u6216\u540c\u7b49\u7522\u54c1\uff09<\/p>\n

\u5132\u5b58\u689d\u4ef6 (Storage Conditions ) \u548c\u8a66\u5291\u6e96\u5099 (Reagent Preparation)<\/strong>
\n\u7522\u54c1\u5132\u5b58\u5728-20\u00b0C\uff0c\u907f\u5149\u3002\u00a0COX-1 Positive Control (K549-100-6) \uff1a\u752820\u03bcl\u7121\u83ccddH2O (sterile ddH2O)\u91cd\u69cb\u3002 \u5206\u88dd\u4e26\u5132\u5b58\u5728-80\u00b0C\u3002 \u907f\u514d\u53cd\u8986\u51cd\u878d\u3002 \u5728\u5169\u500b\u6708\u5167\u4f7f\u7528\u5b8c\u3002 \u5c0d\u65bc\u77ed\u671f\u5132\u5b58\uff08\u7d042\u9031\uff09\u967d\u6027\u5c0d\u7167\u53ef\u4ee5\u5132\u5b58\u5728-20\u00b0C (\u77ed\u671f\u5132\u5b58) \u3002 Positive Control\u5728\u4f7f\u7528\u6642\u4fdd\u6301\u5728\u51b0\u4e0a\u4f7f\u7528\uff0c \u7a69\u5b9a\u6027\u81f3\u5c11~30\u5206\u9418\u3002 \u6ce8\u610f\uff1a\u4e0d\u8981\u5c07\u9176\u9577\u6642\u9593\u4fdd\u5b58\u5728\u51b0\u4e0a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK549-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of COX activity in various biological samples and purified\/crude enzyme preparations<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient assay to measure both COX-1 and COX-2 activity
\n\u2022 High-throughput adaptable
\n\u2022 Includes COX-1 inhibitor, SC560 & COX-2 Inhibitor, Celecoxib<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 COX Assay Buffer
\n\u2022 COX Probe (in DMSO)
\n\u2022 COX Cofactor (in DMSO)
\n\u2022 Arachidonic Acid
\n\u2022 NaOH
\n\u2022 COX-1, Ovine
\n\u2022 SC560, COX-1 inhibitor (in DMSO)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6a23\u54c1\u88fd\u5099\u6ce8\u610f\u4e8b\u9805<\/strong><\/p>\n

\u65b0\u9bae\u6a23\u54c1\u7e3d\u662f\u512a\u65bc\u51b7\u51cd\u6a23\u54c1\u3002 \u82e5\u4f7f\u7528\u51b7\u51cd\u6a23\u54c1\uff0c\u5efa\u8b70\u5206\u96e2\u5f8c\u7acb\u5373\u51b7\u51cd\uff0c\u4e0d\u8981\u591a\u6b21\u51cd\u878d\uff08\u5efa\u8b70\u5728\u51b7\u51cd\u524d\u5c07\u6a23\u54c1\u7b49\u5206\uff09, \u51b7\u51cd\u6642\u9593\u4e0d\u5b9c\u904e\u9577\u3002\u5efa\u8b70\u4f7f\u7528\u704c\u6ce8\u7d44\u7e54\u6a23\u672c\u4f86\u88fd\u5099\u7d44\u7e54\u52fb\u6f3f\u3002\u5c0d\u65bc\u672a\u77e5\u6a23\u54c1\uff0c\u6211\u5011\u5efa\u8b70\u9032\u884c\u8a66\u9a57\u6027\u5be6\u9a57\u4e26\u6e2c\u8a66\u5e7e\u500b\u5291\u91cf\u4ee5\u78ba\u4fdd\u8b80\u6578\u5728\u6a19\u6e96\u66f2\u7dda\u7bc4\u570d\u3002<\/p>\n

\u64cd\u4f5c\u65b9\u6cd5-<\/strong>\u6b63\u78ba\u7248\u672c\u8acb\u4f9d\u7167\u539f\u5ee0\u64cd\u4f5c\u6307\u793a, \u4ee5\u4e0b\u65b9\u6cd5\u50c5\u63d0\u4f9b\u53c3\u8003\u3002<\/p>\n

\u4e00\u3001\u6a23\u54c1\u88fd\u5099<\/strong><\/p>\n

    \n
  1. (A) \u7d30\u80de\u88c2\u89e3\u6db2 (cell lysate) \u88fd\u5099:<\/strong> \u75281\u500d\u4e4b10ml <\/span>PBS\u6d17\u6ecc\u7d30\u80de\uff08~2-6 X 106<\/sup>\uff09\u4e00\u6b21\u3002\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u65bc1 ml<\/span><\/span> PBS\uff081X\uff09\u4e2d\uff0c\u4e26\u5c07\u7d30\u80de\u8f49\u79fb\u52301.5ml<\/span>\u5fae\u91cf\u96e2\u5fc3\u7ba1\u3002 \u4ee5500\u00d7g\u96e2\u5fc33\u5206\u9418\u5f8c\u3002 \u68c4\u53bb\u4e0a\u6e05\u6db2 (supernatant) \u5f8c\uff0c\u5c07\u7d30\u80de\u6c89\u6fb1\u7269\u90e8\u5206 (cell pellet) \u91cd\u61f8\u65bc0.2-0.5ml<\/span>\u4e4b\u88c2\u89e3\u6db2\u7de9\u885d\u6db2 (Lysis buffer)\u4e2d\u542b\u6709\u86cb\u767d\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\u7de9\u885d\u6db2 (\u00a0protease inhibitor cocktail )\u3002 Vortex\u4e26\u51b0\u4e0a\u53cd\u61c95\u5206\u9418\u3002<\/li>\n
  2. (B) \u7d44\u7e54\u52fb\u6f3f\u88fd\u5099 (\u00a0tissue homogenate):\u00a0<\/strong>\u75281\u500dPBS\uff081X\uff09\u6e05\u6d17\u7d44\u7e54\uff08~50-100mg wet\uff09\u4e09\u6b21\u3002 \u52a0\u51650.2-0.5ml<\/span>\u542b\u86cb\u767d\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\u7684\u88c2\u89e3\u7de9\u885d\u6db2\uff08\u672a\u63d0\u4f9b\uff09\u4e26\u65bc\u51b0\u4e0a\u5feb\u901f\u5747\u8cea\u5316\u7d44\u7e54\u3002 \u5c07\u7d30\u80de\u88c2\u89e3\u7269 (A) \u6216\u7d44\u7e54\u52fb\u6f3f(A) \u572812,000\u00d7g\uff0c4\u2103\u4e0b\u96e2\u5fc33\u5206\u9418\u3002 \u6536\u96c6\u4e0a\u6e05\u6db2\u4e26\u4fdd\u6301\u5728\u51b0\u4e0a\u3002\u65b0\u9bae\u6a23\u54c1\u7e3d\u662f\u512a\u65bc\u51b7\u51cd\u6a23\u54c1\u3002 \u82e5\u4f7f\u7528\u51b7\u51cd\u6a23\u54c1\uff0c\u5efa\u8b70\u5206\u96e2\u5f8c\u7acb\u5373\u5206\u88dd\u51b7\u51cd\uff0c\u4e0d\u8981\u591a\u6b21\u51cd\u878d\uff08\u5efa\u8b70\u5728\u51b7\u51cd\u524d\u5c07\u6a23\u54c1\u7b49\u5206\uff09\u51b7\u51cd\u6642\u9593\u4e0d\u5b9c\u904e\u9577\u3002\u5efa\u8b70\u4f7f\u7528\u704c\u6ce8\u7d44\u7e54\u6a23\u672c\u4f86\u88fd\u5099\u7d44\u7e54\u52fb\u6f3f (\u6700\u4f73\u5316)\u3002\u5c0d\u65bc\u672a\u77e5\u6a23\u54c1\uff0c\u6211\u5011\u5efa\u8b70\u9032\u884c\u8a66\u9a57\u6027\u5be6\u9a57\u4e26\u6e2c\u8a66\u5e7e\u500b\u5291\u91cf\u4ee5\u78ba\u4fdd\u8b80\u6578\u5728\u6a19\u6e96\u66f2\u7dda\u7bc4\u570d\u3002<\/li>\n<\/ol>\n

    \u6ce8\u610f\u4e8b\u9805: \u5957\u7d44\u672a\u63d0\u4f9bLysis buffer (\u4f8b\u59821\u00d7\u542b1\uff05NP40\u7684PBS) \u53caprotease inhibitor cocktail\u3002<\/p>\n

    \u4e8c\u3001\u6a19\u6e96\u66f2\u7dda (Resorufin Standard, K549-100-7) \u88fd\u5099<\/strong><\/p>\n

      \n
    1. \u5c07Resorufin \u6a19\u6e96\u54c1\u00a0\u7a00\u91cb\u81f310\u03bcM\uff0810 pmol \/\u03bcl\uff09: \u53d62\u03bcl <\/span>Resorufin \u6a19\u6e96\u54c1 (5 mM, in DMSO)\u81f3 998\u03bcl <\/span><\/span>COX\u5206\u6790\u7de9\u885d\u6db2\u4e2d\uff0c\u5c07Resorufin \u6a19\u6e96\u54c1\u7a00\u91cb\u81f310\u03bcM<\/span><\/span>\uff0810 pmol \/\u03bcl)\uff0c\u53d6\u4e0a\u9762\u914d\u88fd\u597d\u4e4b\u00a010\u03bcM Resorufin\u00a0\u6a19\u6e96\u54c1 (10uM)\u00a050\u03bcl<\/span><\/span> \u65bc450\u03bcl<\/span><\/span>COX\u5206\u6790\u7de9\u885d\u6db2\u4e2d\uff0c\u9032\u4e00\u6b65\u7a00\u91cb\u81f31\u03bcM<\/span>\uff081 pmol \/\u03bcl\uff09\u4e4bResorufin\u00a0\u6a19\u6e96\u54c1\u3002\u5c070,4,8,12,16\u548c20\u03bcl\u76841 pmol \/\u03bclSororufin\u6a19\u6e96\u54c1\u52a0\u5165\u523096\u5b54\u677f\u4e2d\u7684\u4e00\u7cfb\u5217\u5b54\u4e2d\uff0c\u4ee5\u7522\u751f0,4,8,12,16\u548c20
      \npmol \/ well\u4e4bResorufin \u6a19\u6e96\u54c1\u3002 \u4f7f\u7528COX Assay Buffer\u5c07\u9ad4\u7a4d\u8abf\u7bc0\u81f3100\u03bcl\/\u5b54 (100ul, 96ul, 92ul, 88ul, 84ul, 80ul)<\/span>\u3002\u7a00\u91cb\u5f8c\u4e4b\u6a19\u6e96\u54c1\u4e0d\u5efa\u8b70\u518d\u6b21\u4e0b\u500b\u5be6\u9a57\u4f7f\u7528\u3002<\/li>\n<\/ol>\n

      \u4e09\u3001 COX Activity\u53cd\u61c9\u6db2\u88fd\u5099\u00a0<\/strong><\/p>\n

        \n
      1. COX Cofactor\u00a0 200\u500d\u7a00\u91cb\u4f7f\u7528\uff1a\u5728\u4f7f\u7528\u7522\u54c1\u4e4b\u524d\uff0c\u5c07COX Cofactor ( 200\u500d\u7a00\u91cb\u4f7f\u7528)\u3002\u52a02\u03bclCOX\u8f14\u56e0\u5b50 (K549-100-3) \u65bc398\u03bclCOX\u5206\u6790\u7de9\u885d\u6db2\u4e2d (K549-100-1 ) (COX Cofactor\u00a0200\u500d\u7a00\u91cb)\u6df7\u5408\u5747\u52fb\u3002<\/li>\n
      2. \u82b1\u751f\u56db\u70ef\u9178\u6eb6\u6db2 (Arachidonic Acid solution)\u88fd\u5099: \u5728\u4f7f\u7528\u524d\uff0c\u53d65\u03bclNaOH (K549-100-5 ) \u82075\u03bcl \u82b1\u751f\u56db\u70ef\u9178 (Arachidonic Acid, K549-100-4 ) \u88fd\u5099\u6210\u82b1\u751f\u56db\u70ef\u9178\u6eb6\u6db2 (Arachidonic Acid solution)\u3002 \u77ed\u66abVortex\u6df7\u5408\u3002\u53d6\u4e0a\u9762\u914d\u88fd\u597d\u4e4b\u7a00\u91cb\u82b1\u751f\u56db\u70ef\u9178\/ NaOH\u6eb6\u6db2\uff0c\u52a0\u516590\u03bclddH2O\uff0c\u4ee510\u500d\u7a00\u91cb\u4f7f\u7528\uff0c\u77ed\u66abVortex\u6df7\u5408\u3002 (\u6839\u64da\u4f7f\u7528\u91cf\u9700\u589e\u52a0\u914d\u88fd\u91cf) \u3002<\/li>\n
      3. \u5c0d\u65bcPositive Control\u967d\u6027\u5c0d\u7167\u548c\u6bcf\u500b\u6a23\u54c1\uff0c\u6e96\u50992\u500b\u5e73\u884c\u5b54\u3002 \u5411\u5176\u4e2d\u4e00\u500b\u5b54\u4e2d\u52a0\u51652\u03bcl DMSO\uff08\u7528\u65bc\u7e3d\u6d3b\u6027\uff09\u548c\u5176\u4ed6\u5b54\u52a0\u51652\u03bcl COX-1\u6216COX-2\u6291\u5236\u5291\u3002 \u8981\u6e2c\u91cfCOX-1\u6d3b\u6027\uff0c\u6dfb\u52a0COX-1\u6291\u5236\u5291\uff08SC560\uff09\u4e26\u6e2c\u91cfCOX-2\u6d3b\u6027\uff0c\u52a0\u5165COX-2\u6291\u5236\u5291\uff08Celecoxib\uff09\u3002 \u4ee5\u4e0b\u70ba2\u500b\u53cd\u61c9\u6df7\u5408\u7269\u4e4b\u91cf\uff1a<\/li>\n<\/ol>\n

        \"\"<\/p>\n

        \u572896\u5b54\u677f\u7684\u6bcf\u500b\u5e73\u884c\u5b54\u4e2d\u52a0\u516588\u03bcl\u53cd\u61c9\u6df7\u5408\u7269 (Reaction Mix ) \u3002 \u5c0d\u65bc\u967d\u6027\u5c0d\u7167\uff0c\u75282\u03bcl Positive Control \u66ff\u63db\u6a23\u54c1\u53cd\u61c9\u6df7\u5408\u7269\u3002 \u4f7f\u7528pipette \u5728\u6bcf\u500b\u5b54\u4e2d\u52a0\u516510\u03bcl\u7a00\u91cb\u7684\u82b1\u751f\u56db\u70ef\u9178\/ NaOH\u6eb6\u6db2\u540c\u6642\u5f15\u767c\u53cd\u61c9\u3002<\/p>\n

        \u6ce8\u610f\u4e8b\u9805:\u00a0<\/span> \u7a00\u91cb\u5f8c\u7684COX\u8f14\u56e0\u5b50 (\u00a0Diluted COX Cofactor) \u5728\u5ba4\u6eab\u4e0b\u7a69\u5b9a1\u5c0f\u6642\u3002 \u4e0d\u5efa\u8b70\u5132\u5b58\u7a00\u91cb\u7684COX\u8f14\u52a9\u56e0\u5b50\u5f8c\u518d\u4f7f\u7528\u3002\u7a00\u91cb\u7684\u82b1\u751f\u56db\u70ef\u9178\/ NaOH\u6eb6\u6db2\u5728\u51b0\u4e0a\u7a69\u5b9a\u81f3\u5c111\u5c0f\u6642\u3002 \u4e0d\u5efa\u8b70\u5132\u5b58\u7a00\u91cb\u7684\u82b1\u751f\u56db\u70ef\u9178
        \n\u9178\/ NaOH\u6eb6\u6db2\u5f8c\u518d\u4f7f\u7528\u3002<\/p>\n

        \u56db\u3001 \u6e2c\u91cf<\/strong><\/p>\n

        \u52a0\u5165\u82b1\u751f\u56db\u70ef\u9178 (Arachidonic Acid) \u5f8c\uff0c\u7acb\u5373\u4ee5\u52d5\u529b\u5b78\u6a21\u5f0f\u6e2c\u91cf\u87a2\u5149\uff08Ex \/ Em = 535\/587 nm\uff09\u6bcf15\u79d2\u3002 \u6301\u7e8c30\u5206\u9418
        \n\u6ce8\u610f\uff1a\u53cd\u61c9\u6642\u9593\u53d6\u6c7a\u65bc\u6a23\u54c1\u7684COX\u6d3b\u6027\u3002 \u5efa\u8b70\u5728\u52d5\u529b\u5b78\u6a21\u5f0f\u4e0b\u6e2c\u91cf\u87a2\u5149\u4e26\u5728\u7dda\u6027\u7bc4\u570d\u5167\u9078\u64c7\u5169\u7a2e\u6642\u9593\u9ede\uff08T1\u548cT2\uff09\uff0c\u4ee5\u8a08\u7b97\u6a23\u54c1\uff08RFUS\uff09\u548c\u542b\u6291\u5236\u5291\u6a23\u54c1\uff08RFUI\uff09\u7684COX\u6d3b\u6027\u3002\u8a72\u6a19\u6e96\u66f2\u7dda\u53ef\u4ee5\u5728\u7d42\u9ede\u6a21\u5f0f\u4e0b\u8b80\u53d6\uff08\u5373\u5728\u53cd\u61c9\u6642\u9593\u7d50\u675f\u6642\uff09\u3002<\/p>\n

        \u4e94\u3001\u8a08\u7b97\uff1a\u5f9e\u6240\u6709\u6a19\u6e96\u8b80\u6578\u4e2d\u6e1b\u53bb0\u6a19\u6e96\u8b80\u6578\u3002 \u7e6a\u88fdResorufin\u6a19\u6e96\u66f2\u7dda\u3002 \u8a08\u7b97\u6a23\u672c\u7684COX\u6d3b\u6027
        \n\u0394RFU=\uff08RFUS2-RFUS1\uff09 – \uff08RFUI2-RFUI1\uff09\u3002 \u5c07\u0394RFU\u61c9\u7528\u65bcResorufin\u6a19\u6e96\u66f2\u7dda\uff0c\u5f97\u5230B pmol\u7684Resorufin\u5728\u53cd\u61c9\u6642\u9593\u5167\u7531\u5404\u81ea\u7684COX\u540c\u5de5\u9176\u7522\u751f\uff08\u0394T= T2-T1\uff09\u3002<\/p>\n

        \"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

        \u74b0\u6c27\u5408\u9176\uff08COX\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09\u00a0|\u00a0\u8ca8\u865fK549-100 Cyclooxygenase (COX) […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2515],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18073"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18073"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18073\/revisions"}],"predecessor-version":[{"id":19621,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18073\/revisions\/19621"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18073"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18073"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18073"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}