{"id":17983,"date":"2018-09-29T15:50:47","date_gmt":"2018-09-29T07:50:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17983"},"modified":"2022-07-20T15:30:33","modified_gmt":"2022-07-20T07:30:33","slug":"goat-anti-human-ig-alexa-fluor-647-southernbiotech%e8%b2%a8%e8%99%9f2010-31","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17983","title":{"rendered":"Goat Anti-Human Ig-Alexa Fluor\u00ae 647 | SouthernBiotech\u8ca8\u865f2010-31"},"content":{"rendered":"

Goat Anti-Human Ig-Alexa Fluor\u00ae 647 \u00a0| <\/strong>\u8ca8\u865f<\/strong>2010-31 \u00a0\u00a0<\/strong>\u898f\u683c<\/strong>1.0 mL \u00a0(1.0 mg \/ mL)<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Goat Anti-Human IgG\uff0c\u4f86\u6e90\u70baPooled antisera from goats hyperimmunized with human IgG, IgM, and IgA\u3002\u7279\u7570\u6027\u8fa8\u8996of human IgG, IgM, and IgA s\u4e4b\u91cd\u93c8heavy \u3001 \u8207\u8f15\u93c8light chains\u3002\u6b64\u6297\u9ad4Affinity chromatography on immunoglobulins covalently linked to agarose\u7d14\u5316, \u5177AF647 (Alexa Fluor\u00ae 647)\u6a19\u8a8c\u3002\u53ef\u61c9\u7528\u65bc\u5404\u7a2e\u514d\u75ab\u5206\u6790\u7684\u8a66\u9a57\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with the heavy and light chains of human IgG, IgM, and IgA<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with human IgG, IgM, and IgA<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nNone; may react with immunoglobulins from other species<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on pooled human immunoglobulins covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nGoat IgG-Alexa Fluor\u00ae<\/sup>\u00a0647<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nAF647 (Alexa Fluor\u00ae<\/sup>\u00a0647)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nSupplied in PBS\/NaN3<\/sub><\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA\u00a01-8<\/sup>
\nFLISA
\nFlow Cytometry\u00a015,16,18<\/sup><\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

Immunocytochemistry\u00a09<\/sup>
\nWestern Blot\u00a010-13<\/sup>
\nMultiplex\u00a014<\/sup>
\nStimulation\u00a015-17<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

    \n
  1. Kouzmitcheva GA, Petrenko VA, Smith GP. Identifying diagnostic peptides for Lyme disease through epitope discovery. Clin Diagn Lab Immunol. 2001;8:150-60. (ELISA)<\/li>\n
  2. Pancook JD, Beuerlein G, Pecht G, Tang Y, Nie Y, Wu H, et al. In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens. Hybrid Hybridomics. 2001;20:383-96. (ELISA)<\/li>\n
  3. Weitkamp J, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, et al. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods. 2003;275:223-37. (ELISA)<\/li>\n
  4. Ohdan H, Zhou W, Tanaka Y, Irei T, Fuchimoto Y, Egawa H, et al. Evidence of immune tolerance to blood group antigens in a case of ABO-incompatible pediatric liver transplantation. Am J Transplant. 2007;7:2190-4. (ELISA)<\/li>\n
  5. Williams JV, Weitkamp J, Blum DL, LaFleur BJ, Crowe JE Jr. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol. 2009;47:407-14. (ELISA)<\/li>\n
  6. Fan Q, Longnecker R. The Ig-like V-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion. J Virol. 2010;84:8664-72. (ELISA)<\/li>\n
  7. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (ELISA)<\/li>\n
  8. Mart\u00ed GP, Mohn KG, Cox RJ, Brokstad KA. The influence of tonsillectomy on total serum antibody levels. Scand J Immunol. 2014;80:377-9. (ELISA)<\/li>\n
  9. Moody MA, Yates NL, Amos JD, Drinker MS, Eudailey JA, Gurley TC, et al. HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages. J Virol. 2012;86:7496-507. (ICC)<\/li>\n
  10. Moritz DR, Gheyselinck J, Klemenz R. Expression analysis of the soluble and membrane-associated forms of the interleukin-1 receptor-related Tl protein in primary mast cells and fibroblasts. Hybridoma. 1998;17:107-16. (WB)<\/li>\n
  11. Davenport V, Guthrie T, Findlow J, Borrow R, Williams NA, Heyderman RS. Evidence for naturally acquired T cell-mediated mucosal immunity to Neisseria meningitidis. J Immunol. 2003;171:4263-70. (WB)<\/li>\n
  12. Botelho-Nevers E, Gouriet F, Lepidi H, Couvret A, Amphoux B, Dessi P, et al. Chronic nasal infection caused by Klebsiella rhinoscleromatis or Klebsiella ozaenae: two forgotten infectious diseases. Int J Infect Dis. 2007;11:423-9. (WB)<\/li>\n
  13. Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013;190:1591-602. (WB)<\/li>\n
  14. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)<\/li>\n
  15. Fruehling S, Lee SK, Herrold R, Frech B, Laux G, Kremmer E, et al. Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. J Virol. 1996;70:6216-26. (FC, Stim)<\/li>\n
  16. Fruehling S, Swart R, Dolwick KM, Kremmer E, Longnecker R. Tyrosine 112 of latent membrane protein 2A is essential for protein tyrosine kinase loading and regulation of Epstein-Barr virus latency. J Virol. 1998;72:7796-806. (FC, Stim)<\/li>\n
  17. Koethe S, Zander L, K\u00f6ster S, Annan A, Ebenfelt A, Spencer J, et al. Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011;90:5-19. (Stim)<\/li>\n
  18. Wiehr S, B\u00fchler P, Gierschner D, Wolf P, Rolle A, Kesenheimer C, et al. Pharmacokinetics and PET imaging properties of two recombinant anti-PSMA antibody fragments in comparison to their parental antibody. Prostate. 2014;74:743-55. (FC)<\/li>\n<\/ol>\n

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