{"id":17924,"date":"2018-09-18T16:34:16","date_gmt":"2018-09-18T08:34:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17924"},"modified":"2022-07-20T15:55:16","modified_gmt":"2022-07-20T07:55:16","slug":"bcl6-lentiviral-vector%e6%85%a2%e7%97%85%e6%af%92%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9flv808039","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17924","title":{"rendered":"Bcl6 Lentiviral Vector\u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865fLV808039"},"content":{"rendered":"

Bcl6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)\u00a0<\/strong>\u6162\u75c5\u6bd2\u8f09\u9ad4<\/strong> | <\/strong>\u8ca8\u865f<\/strong>LV808039<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>Bcl6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) <\/u>\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4\u3002BCL6\u8868\u9054\u5c07\u7531\u5177\u6709GFP\u5831\u544a\u57fa\u56e0\u7684CMV\u555f\u52d5\u5b50\u9a45\u52d5\u3002 \u63d2\u5165\u7269\u7684\u5169\u5074\u662f\u4e26\u4e14\u53ef\u4ee5\u4f7f\u7528NheI\u548cBamHI\u5207\u9664\uff0c\u53ea\u8981\u63d2\u5165\u7269\u4e0d\u542b\u4efb\u4f55\u5167\u90e8NheI\u6216BamHI\u4f4d\u9ede\u5373\u53ef\u3002Bcl6<\/u>\u57fa\u56e0 (Accession Number NM_001130845<\/a>) \u67b6\u69cb\u65bcpLenti-GIII-CMV-GFP-2A-Puro<\/a>\u8868\u73fe\u8f09\u9ad4\uff0c\u7531CMV\u555f\u52d5\u5b50\u9a45\u52d5\uff0c \u5177GFP Reporter\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u9032\u884c\u8f49\u67d3\u7528\u65bc\u5373\u6642\u8868\u9054\uff0c\u6216\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\u7528\u65bc\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u8868\u9054\u3002\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u5efa\u8b70\u4f7f\u7528ABMGOOD<\/strong>\u63d0\u4f9b\u4e4b\u8f49\u67d3\u8a66\u5291<\/strong> Cat#\u00a0G074, Lentifectin\u2122<\/a> \u53ca\u512a\u5316\u4e4b\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217LV003<\/a>\u3002\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1\u5305\u62ecLentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\/\u00a0Lentivirus <\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\u4e26\u4ee5\u00a0Lentivirus<\/u>\u75c5\u6bd2<\/u>RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0\u7d14\u5316\u5f8c\u4e4b\u75c5\u6bd2\u529b\u50f9\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nThe BCL6 expression will be driven by a CMV promoter, with a GFP reporter. Inserts are flanked by and can be excised using NheI and BamHI as long as inserts do not contain any internal NheI or BamHI sites.<\/td>\n<\/tr>\n
Procedure Overview<\/strong><\/td>\n<\/td>\n<\/tr>\n
Product Application<\/strong><\/td>\n\n

\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n

\u00b7\u00a0 Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n

Gene Insert<\/strong><\/td>\nBcl6<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_001130845<\/a>1,2,3,4,5<\/sup><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-GIII-CMV-GFP-2A-Puro<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n8829bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n2121 bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Tags<\/strong><\/td>\nGFP<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nCMV<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nLentiviral Plasmid<\/td>\n<\/tr>\n
Viral Packaging<\/strong><\/td>\nabm<\/strong>\u00a0recommends using Cat#\u00a0G074, Lentifectin\u2122<\/a>\u00a0in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat#\u00a0LV003, 2nd Generation Packaging Mix<\/a>. Alternatively, you can also use Cat#\u00a0LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6162\u75c5\u6bd2\u6280\u8853\u4e4b\u9818\u5c0e\u8005abm\uff0c\u958b\u767c\u4e86\u4e00\u5957\u5168\u9762\u7684\u4eba\u985e\u3001\u5c0f\u9f20\u548c\u5927\u9f20\u57fa\u56e0\u6587\u5eab\uff0cClone\u5230\u6162\u75c5\u6bd2\u8f09\u9ad4\u6216\u88fd\u6210\u5373\u7528\u578b\u6162\u75c5\u6bd2\uff0c\u53ef\u7528\u65bc\u64cd\u7e31\u611f\u8208\u8da3\u57fa\u56e0\u65bc\u5bbf\u4e3b\u7d30\u80de\u8868\u9054\u3002\u70ba\u4e86\u4fc3\u9032\u6211\u5011\u7684\u6162\u75c5\u6bd2\u8f09\u9ad4\u7684\u61c9\u7528\u548c\u591a\u529f\u80fd\u6027\uff0cABM\u6162\u75c5\u6bd2\u8868\u9054\u7cfb\u7d71\u5177\u6709\u8a31\u591a\u7d44\u6210\u578b\u555f\u52d5\u5b50\uff0c\u6a19\u7c64\u5e8f\u5217\u548c\u5831\u5c0e\u57fa\u56e0\u4e4b\u7d44\u5408\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Bcl6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-G […]<\/p>\n","protected":false},"author":3,"featured_media":1089,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[116],"tags":[2548],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17924"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17924"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17924\/revisions"}],"predecessor-version":[{"id":17926,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17924\/revisions\/17926"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1089"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17924"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17924"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17924"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}