{"id":17901,"date":"2018-09-14T10:59:35","date_gmt":"2018-09-14T02:59:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17901"},"modified":"2022-07-20T15:57:58","modified_gmt":"2022-07-20T07:57:58","slug":"plasmidcosmid-%e8%b3%aa%e9%ab%94dna%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9frb94-ypd250","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17901","title":{"rendered":"plasmid\/cosmid  \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44 | T-PRO\u8ca8\u865f#RB94-YPD250"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17901\"><strong>plasmid DNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865fRB94-YPD250<\/strong><\/a><\/span><\/p>\n<p>T-Pro\u8cea\u9ad4\u8a66\u5291\u76d2\u7cfb\u5217\u5546\u54c1\uff0cT-Pro Mini Prep\u7cfb\u7d71\uff08mini Column\uff09\u7d93\u904e\u512a\u5316\u8655\u7406\uff1b\u5feb\u901f\u5206\u96e2plasmid\u8cea\u9ad4DNA \u6216cosmid DNA\uff1b\u9ad4\u7a4d\u70ba1-5ml\u7d30\u83cc\u57f9\u990a\u6db2\u3002\u5927\u91cf\u88fd\u5099\u9078\u64c7T-Pro Midi \/ Maxi\uff0c\u5176\u7522\u91cf\u70ba500\u03bcg-1mg\u3002\u6b64\u8403\u53d6\u8cea\u9ad4DNA\u7684\u65b9\u6cd5\uff0c\u70ba\u4f7f\u7528\u4fee\u98fe\u7684\u9e7c\u6027\u88c2\u89e3\u6cd5(<strong>alkaline lysis method)<\/strong>\uff0c\u63a5\u8457\u9032\u884cRNase\u8655\u7406\u964d\u4f4egDNA\u6216RNA\u6c61\u67d3\uff0c\u4ee5\u7372\u5f97\u6f84\u6e05\u7684\u7d30\u80de\u88c2\u89e3\u7269\u3002<strong>Silica spin\u00a0<\/strong>\u6280\u8853\u8207<strong>chaotrophic salt<\/strong><strong>\u4e4b<\/strong>\u7d50\u5408\uff0c\u63d0\u4f9b\u4e86\u53ef\u9760\u7684DNA\u7d50\u5408\u548c<strong>elution<\/strong>\u7cfb\u7d71\u3002\u7d14\u5316\u7684DNA\uff0c\u53ef\u76f4\u63a5\u61c9\u7528\u65bc\u4e0b\u6e38\u5206\u751f\u5be6\u9a57: \u7528\u65bcdigestion\u3001 ligestion\u3001PCR\u53ca\u5b9a\u5e8f\u53cd\u61c9\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/11\/S__7675950-300x225.jpg\" alt=\"S__7675950\" \/><\/p>\n<p><strong style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7101\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2017\/09\/tag6-e1505358740644-300x106.jpg\" alt=\"\u76f8\u95dc\u8a66\u5291\" width=\"95\" height=\"36\" \/><\/a><\/strong>\u00a0<strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=18163\">WPA\u83cc\u6db2\u5bc6\u5ea6\u6e2c\u91cf\u5100\u5668<\/a>|\u00a0<\/span><\/strong>\u00a0<strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=safeview-%E6%96%B0%E5%9E%8B%E6%A0%B8%E9%85%B8%E5%AE%89%E5%85%A8%E6%9F%93%E5%8A%91\">\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=18156\">Agarose<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=2671\">\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=451\">\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17901\">\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=100\">CMOS\u76f8\u6a5f<\/a>\u00a0|<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6196\">Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|\u00a0<a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=3880\">\u684c\u4e0a\u578b\u6ec5\u83cc\u91dc<\/a><\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>plasmid DNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865fRB94-YPD250 T-Pro\u8cea\u9ad4\u8a66\u5291\u76d2\u7cfb\u5217\u5546\u54c1\uff0cT-Pro Mi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":7902,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[2555],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17901"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17901"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17901\/revisions"}],"predecessor-version":[{"id":18185,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17901\/revisions\/18185"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7902"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17901"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17901"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17901"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}