{"id":17868,"date":"2018-09-10T14:27:33","date_gmt":"2018-09-10T06:27:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17868"},"modified":"2022-10-27T16:05:21","modified_gmt":"2022-10-27T08:05:21","slug":"%e9%b9%bc%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6%ef%bc%88alp%ef%bc%89%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9fk412-500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17868","title":{"rendered":"\u9e7c\u6027\u78f7\u9178\u9176\uff08ALP\uff09\u6d3b\u6027\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK412-500(ABCAM ab83369)"},"content":{"rendered":"

\u9e7c\u6027\u78f7\u9178\u9176\uff08ALP\uff09\u6d3b\u6027\u5206\u6790\u5957\u7d44\uff08\u03bbmax= 405nm\uff09 | <\/strong>\u8ca8\u865f<\/strong>K412-500(ABCAM ab83369)<\/a><\/span><\/p>\n

Alkaline Phosphatase Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u9e7c\u6027\u78f7\u9178\u9176\uff08Alkaline Phosphatase, ALP\uff09\u50ac\u5316\u9e7c\u6027\u7de9\u885d\u6db2\u4e2d\u78f7\u9178\u916f\u7684\u6c34\u89e3\uff0c\u7522\u751f\u6709\u6a5f\u81ea\u7531\u57fa\u548c\u7121\u6a5f\u78f7\u9178\u9e7d\u3002 ALP\u9e7c\u6027\u78f7\u9178\u9176\u6c34\u5e73\u548c\u6d3b\u6027\u7684\u8b8a\u5316\u8207\u809d\u81df\u548c\u9aa8\u9abc\u4e2d\u7684\u5404\u7a2e\u75be\u75c5\u72c0\u614b\u76f8\u95dc\u3002 BioVision\u7684\u9e7c\u6027\u78f7\u9178\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\uff0c\u7c21\u55ae\uff0c\u76f4\u63a5\u548cHTS\u6bd4\u8272\u5206\u6790\uff0c\u65e8\u5728\u6e2c\u91cf\u8840\u6e05\u548c\u751f\u7269\u6a23\u54c1\u4e2d\u7684ALP\u6d3b\u6027\u3002 \u5b83\u542b\u670910 substrate tablets\uff0c\u65b9\u4fbf\u591a\u7a2e\u7528\u9014\u3002 \u8a72\u8a66\u5291\u76d2\u4f7f\u7528\u78f7\u9178\u5c0d\u785d\u57fa\u82ef\u916f\uff08pNPP\uff09\u4f5c\u70ba\u78f7\u9178\u9176\u5e95\u7269\uff0c\u7576\u901a\u904eALP\u53bb\u78f7\u9178\u5316\u6642\uff0c\u5176\u8b8a\u9ec3\uff08\u03bbmax= 405nm\uff09\u3002 \u8a66\u5291\u76d2\u6aa2\u6e2c\u9748\u654f\u5ea610-250\u03bcU\u7684ALP\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK412-500<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n500 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (405 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Kit can detect 10-250 \u00b5U ALP in samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 3 hours
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive and stable
\n\u2022 The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 ALP Assay Buffer
\n\u2022 pNPP (10 TAB)
\n\u2022 ALP Enzyme
\n\u2022 Stop Solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
Mohamed Aboul Ezz et al., (2017) The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure,\u00a0Reproductive Biology, 2017, 17: 42\u201350<\/a><\/div>\n<\/div>\n
\n
Lee, Sung-Il et al. (2017) Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva\u2011derived stem cells and osteoprecursor cells,\u00a0Exp Ther Med. 2017 Jun;13(6):3467-3473.<\/a><\/div>\n<\/div>\n
\n
Dalia, Ali et al. (2016) Epigenetic Library Screen Identifies Abexinostat as Novel Regulator of Adipocytic and Osteoblastic Differentiation of Human Skeletal (Mesenchymal) Stem Cells.\u00a0Stem Cells Transl Med. 2016 Aug;5(8):1036-47.<\/a><\/div>\n<\/div>\n
\n
Chen et al., PDGFB-based stem cell gene therapy increases bone strength in the mouse.\u00a0PNAS,\u00a0Jul 2015; 112: E3893 – E3900.<\/a><\/div>\n<\/div>\n
\n
Dambatta et al., In vitro degradation and cell viability assessment of Zn\u20133Mg alloy for biodegradable bone implants.\u00a0Journal of Engineering in Medicine, May 2015; 229: 335 – 342.<\/a><\/div>\n<\/div>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u9e7c\u6027\u78f7\u9178\u9176\uff08ALP\uff09\u6d3b\u6027\u5206\u6790\u5957\u7d44\uff08\u03bbmax= 405nm\uff09 | \u8ca8\u865fK412-500(ABCAM ab8336 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2565],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17868"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17868"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17868\/revisions"}],"predecessor-version":[{"id":35491,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17868\/revisions\/35491"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17868"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17868"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17868"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}