{"id":17762,"date":"2018-09-06T13:31:28","date_gmt":"2018-09-06T05:31:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17762"},"modified":"2022-09-20T13:55:40","modified_gmt":"2022-09-20T05:55:40","slug":"5x-protein-loading-buffer-%e8%9b%8b%e7%99%bd%e8%b3%aa%e7%b7%a9%e8%a1%9d%e6%b6%b2-%e8%b2%a8%e8%99%9fg062","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17762","title":{"rendered":"5X Protein Loading Buffer \u86cb\u767d\u8cea\u7de9\u885d\u6db2 | \u8ca8\u865fG031"},"content":{"rendered":"

5X Protein Loading Buffer \u86cb\u767d\u8cea\u7de9\u885d\u6db2 | \u8ca8\u865fG03<\/a>1<\/span><\/p>\n

abm\u76845X\u86cb\u767d\u8ceaLoading\u7de9\u885d\u6db2\u3002 \u5c07\u4e00\u4efd\u6a23\u54c1\u7de9\u885d\u6db2\u8207\u56db\u4efd\u86cb\u767d\u8cea\u6a23\u54c1\u6df7\u5408\uff0c\u52a0\u71b1\u8b8a\u6027\uff0c\u7136\u5f8c\u5c07\u6a23\u54c1\u52a0\u8f09\u5230\u51dd\u81a0\u5b54\u4e2d\u3002 \u901a\u904e\u76e3\u6e2c\u85cd\u8272\u67d3\u6599\u524d\u6cbf\u7684\u4f4d\u7f6e\u4f86\u53ef\u8996\u5316\u96fb\u6cf3\u7684\u9032\u5c55\u3002\u53ef\u7528\u65bc\u5404\u7a2e\u8b8a\u6027\u51dd\u81a0\uff0c\u4e26\u8207coomassie dye\u3001silver staining\u9280\u67d3\u6280\u8853\u3001\u4ee5\u53caWestern blotting\u86cb\u767d\u8cea\u5370\u8de1\u7a0b\u5e8f\u76f8\u5bb9\u30025X loading buffer also acts as a reducing agent for denaturing proteins.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nQuantity<\/strong><\/td>\nCat No.<\/strong><\/td>\n<\/tr>\n
2-Mercaptoethanol<\/a><\/td>\n100 ml<\/td>\nCH044<\/td>\n<\/tr>\n
2-Mercaptoethanol<\/a><\/td>\n200ml (2 x 100ml)<\/td>\nCH045<\/td>\n<\/tr>\n
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs<\/a><\/td>\n50 Tabs<\/td>\nCH027<\/td>\n<\/tr>\n
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs<\/a><\/td>\n100 Tabs<\/td>\nCH028<\/td>\n<\/tr>\n
Actinomycin D<\/a><\/td>\n5.0 mg<\/td>\nCH001<\/td>\n<\/tr>\n
Agarose<\/a><\/td>\n100 g<\/td>\nG060-1<\/td>\n<\/tr>\n
Agarose<\/a><\/td>\n500 g<\/td>\nG060-2<\/td>\n<\/tr>\n
Agarose II, Low Gelling Temperature<\/a><\/td>\n25 g<\/td>\nCH002<\/td>\n<\/tr>\n
Agarose II, Low Gelling Temperature<\/a><\/td>\n100 g<\/td>\nCH003<\/td>\n<\/tr>\n
Albumin, Bovine 20mg\/ml solution<\/a><\/td>\n1.0 ml<\/td>\nCH006<\/td>\n<\/tr>\n
Albumin, Bovine 20mg\/ml solution<\/a><\/td>\n5.0 ml<\/td>\nCH007<\/td>\n<\/tr>\n
Albumin, Bovine pH 5.1 +\/-0.2, Salt Free Form<\/a><\/td>\n25 g<\/td>\nCH004<\/td>\n<\/tr>\n
Albumin, Bovine pH 5.1 +\/-0.2, Salt Free Form<\/a><\/td>\n100 g<\/td>\nCH005<\/td>\n<\/tr>\n
Ammonium Persulfate (APS)<\/a><\/td>\n25 g<\/td>\nCH008<\/td>\n<\/tr>\n
Ammonium Persulfate (APS)<\/a><\/td>\n100 g<\/td>\nCH009<\/td>\n<\/tr>\n
Aprotinin<\/a><\/td>\n10 mg<\/td>\nCH010<\/td>\n<\/tr>\n
Aprotinin<\/a><\/td>\n50 mg<\/td>\nCH011<\/td>\n<\/tr>\n
B-Galactosidase<\/a><\/td>\n1.0 kU<\/td>\nCH036<\/td>\n<\/tr>\n
B-Galactosidase<\/a><\/td>\n5.0 kU<\/td>\nCH037<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate<\/a><\/td>\n100 mg<\/td>\nCH012<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate<\/a><\/td>\n500 mg<\/td>\nCH013<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate Toluidine Salt<\/a><\/td>\n1.0 g<\/td>\nCH014<\/td>\n<\/tr>\n
BCIP\/NBT<\/a><\/td>\n100 ml<\/td>\nCH015<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n50 g<\/td>\nCH016<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n100 g<\/td>\nCH017<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n500 g<\/td>\nCH018<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n5.0 g<\/td>\nG114<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n25 g<\/td>\nCH019<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n50 g<\/td>\nCH020<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n100 g<\/td>\nCH021<\/td>\n<\/tr>\n
Bromophenol Blue, Sodium Salt<\/a><\/td>\n50 g<\/td>\nCH022<\/td>\n<\/tr>\n
Bromophenol Blue, Sodium Salt<\/a><\/td>\n100 g<\/td>\nCH023<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n10 g<\/td>\nCH024<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n25 g<\/td>\nCH025<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n50 g<\/td>\nCH026<\/td>\n<\/tr>\n
Cumate<\/a><\/td>\n500 \u03bcl<\/td>\nCH065<\/td>\n<\/tr>\n
DNA Loading Dye<\/a><\/td>\n6X; 3 x 1.0 ml<\/td>\nG030<\/td>\n<\/tr>\n
DNA Loading Green Dye<\/a><\/td>\n6X; 3 x 1.0 ml<\/td>\nG924<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n5.0 g<\/td>\nCH029<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n10 g<\/td>\nCH030<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n25 g<\/td>\nCH031<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n500 g<\/td>\nCH032<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n1.0 kg<\/td>\nCH033<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n2.5 kg<\/td>\nCH034<\/td>\n<\/tr>\n
Glycerol<\/a><\/td>\n500 ml<\/td>\nG115<\/td>\n<\/tr>\n
Glycerol<\/a><\/td>\n100 ml<\/td>\nCH038<\/td>\n<\/tr>\n
Glycogen<\/a><\/td>\n20 mg\/ml; 1.0 ml<\/td>\nG024<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n25 g<\/td>\nCH039<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n100 g<\/td>\nCH040<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n500 g<\/td>\nCH041<\/td>\n<\/tr>\n
IPTG<\/a><\/td>\n1.0 g<\/td>\nG116<\/td>\n<\/tr>\n
LB Agar<\/a><\/td>\n500 g<\/td>\nG247<\/td>\n<\/tr>\n
LB Broth<\/a><\/td>\n500 g<\/td>\nG246<\/td>\n<\/tr>\n
Neutral Red<\/a><\/td>\n5 x 1.0ml<\/td>\nG035<\/td>\n<\/tr>\n
Nitroblue Tetrazolium Chloride<\/a><\/td>\n500 mg<\/td>\nCH046<\/td>\n<\/tr>\n
Nitroblue Tetrazolium Chloride<\/a><\/td>\n1.0 g<\/td>\nCH047<\/td>\n<\/tr>\n
Oligo(dT)18 Primer<\/a><\/td>\n1.0OD, 500 \u03bcl, 10 \u03bcM<\/td>\nG140<\/td>\n<\/tr>\n
Pepstatin<\/a><\/td>\n5.0 mg<\/td>\nCH048<\/td>\n<\/tr>\n
Polybrene<\/a><\/td>\n1.0 ml; 0.8 mg\/ml<\/td>\nG062<\/td>\n<\/tr>\n
Proteinase K<\/a><\/td>\n1.0 ml<\/td>\nG029<\/td>\n<\/tr>\n
Random Primer (6N)<\/a><\/td>\n1.0OD, 500 \u03bcl, 10 \u03bcM<\/td>\nG139<\/td>\n<\/tr>\n
RNase A Solution<\/a><\/td>\n1.0 ml; 10 mg\/ml<\/td>\nG118<\/td>\n<\/tr>\n
Sodium Azide<\/a><\/td>\n250 g<\/td>\nCH052<\/td>\n<\/tr>\n
Tetracycline, Hydrochloride<\/a><\/td>\n25 g<\/td>\nCH054<\/td>\n<\/tr>\n
Tetracycline, Hydrochloride<\/a><\/td>\n100 g<\/td>\nCH055<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n500 g<\/td>\nCH058<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n1kg (2 x 500g [CH058])<\/td>\nCH059<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n5kg (10 x 500g [CH058])<\/td>\nCH060<\/td>\n<\/tr>\n
Triton X-100, T-Octylphenoxypolyethoxyethanol<\/a><\/td>\n1L (2 x 500ml)<\/td>\nCH061<\/td>\n<\/tr>\n
Triton X-100, T-Octylphenoxypolyethoxyethanol<\/a><\/td>\n4.0 L<\/td>\nCH062<\/td>\n<\/tr>\n
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate<\/a><\/td>\n1L (2 x 500ml)<\/td>\nCH063<\/td>\n<\/tr>\n
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate<\/a><\/td>\n4.0 L<\/td>\nCH064<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

5X Protein Loading Buffer \u86cb\u767d\u8cea\u7de9\u885d\u6db2 | \u8ca8\u865fG031 abm\u76845X\u86cb\u767d\u8ceaLoad […]<\/p>\n","protected":false},"author":3,"featured_media":1090,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[2580],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17762"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17762"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17762\/revisions"}],"predecessor-version":[{"id":17977,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17762\/revisions\/17977"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1090"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17762"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17762"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17762"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}