{"id":17755,"date":"2018-09-06T11:28:27","date_gmt":"2018-09-06T03:28:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17755"},"modified":"2022-07-20T16:03:11","modified_gmt":"2022-07-20T08:03:11","slug":"%e6%8a%97%e5%b0%8f%e9%bc%a0mhc-ii-sprd-sba-clone-nimr-4-%e8%b2%a8%e8%99%9f1895-13","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17755","title":{"rendered":"\u6297\u5c0f\u9f20MHC II-SPRD | SBA Clone NIMR-4 \u8ca8\u865f1895-13"},"content":{"rendered":"

\u6297\u5c0f\u9f20<\/strong>MHC II-SPRD<\/strong> \u00a0| <\/strong>\u8ca8\u865f<\/strong>1895-13\u00a0 \u00a0<\/strong>\u00a0<\/strong><\/a><\/span><\/p>\n

Rat Anti-Mouse MHC Class II-SPRD (Spectral Red\u00ae is a PE\/CY5 tandem conjugate)<\/strong><\/p>\n

\"Rat<\/p>\n

BALB\/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-SPRD (SB Cat. No. 1895-13) and Rat Anti-Mouse CD3\u03b5-PE (SB Cat. No. 1535-09).<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u55ae\u514b\u9686\u6297\u9ad4NIMR-4\u8207MHC II\u985e\u6297\u539f\u4e0a\u7684\u975e\u591a\u614b\u6027I-A\u7de8\u78bc\u8868\u4f4d\u7279\u7570\u6027\u53cd\u61c9\u3002 II\u985e\u6297\u539f\u4e3b\u8981\u5728\u6297\u539f\u5448\u905e\u7d30\u80de\u4e0a\u8868\u9054\uff0c\u5305\u62ecB\u6dcb\u5df4\u7d30\u80de\uff0c\u5de8\u566c\u7d30\u80de\uff0c\u6a39\u7a81\u7d30\u80de\u548c\u67d0\u4e9b\u4e0a\u76ae\u7d30\u80de\u3002Southernbiotech\u63d0\u4f9b\u9ad8\u54c1\u8ceaRat Anti-Mouse MHC Class II-SPRD\u6297\u9ad4\uff0c\u5c0dMouse MHC Class II\u5177\u6709\u5c08\u4e00\u6027\uff0cIsotype\u70baRat IgG2b<\/sub>-SPRD<\/a>\u3002\u6297\u9ad4\u7d14\u5316\u5f8c\u5177\u6709SPRD (Spectral Red\u00ae is a PE\/CY5 tandem conjugate) \u6a19\u8a8c\u3002\u6297\u9ad4\u6eb6\u6db2\u70baSupplied in PBS\/NaN3 and a stabilizing agent\u3002\u8caf\u5b58\u65bc2-8\u00b0C\u30020.1 mg\/mL, 1ml\u5305\u88dd\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nNIMR-4<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nRat IgG2b<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nRat IgG2b<\/sub>-SPRD<\/a><\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nMouse MHC Class II<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nI-A antigen<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nThe monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nSPRD (R-phycoerythrin\/Cyanine 5)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nSupplied in PBS\/NaN3<\/sub>\u00a0and a stabilizing agent<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.1 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a04-13<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a01<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a01,2<\/sup>
\nSeparation \u2013 Reported in literature\u00a03<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

    \n
  1. Andrew EM, Parkhouse RM. Immune induction of Ia antigens in activated T cells and in kidney epithelial cells in mice. Immunology. 1986;58:603-6. (IHC-FS, ICC)<\/li>\n
  2. Zaliauskiene L, Kang S, Sparks K, Zinn KR, Schwiebert LM, Weaver CT, et al. Enhancement of MHC class II-restricted responses by receptor-mediated uptake of peptide antigens. J Immunol. 2002;169:2337-45. (ICC)<\/li>\n
  3. Lindsey JW. Dexamethasone-induced Ras-related protein 1 is a potential regulatory protein in B lymphocytes. Int Immunol. 2007;19:583-90. (Sep)<\/li>\n
  4. Caspar-Bauguil S, Cousin B, Galinier A, Segafredo C, Nibbelink M, Andr\u00e9 M, et al. Adipose tissues as an ancestral immune organ: site-specific change in obesity. FEBS. 2005;579:3487-92. (FC)<\/li>\n
  5. Bauer C, Bauernfeind F, Sterzik A, Orban M, Schnurr M, Lehr HA, et al. Dendritic cell-based vaccination combined with gemcitabine increases survival in a murine pancreatic carcinoma model. Gut. 2007;56:1275-82. (FC)<\/li>\n
  6. Krishnamoorthy N, Oriss TB, Paglia M, Fei M, Yarlagadda M, Vanhaesebroeck B, et al. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat Med. 2008;14:565-73. (FC)<\/li>\n
  7. Gu T, Zhu Y, Chen C, Li M, Chen Y, Yu G, et al. Fine-tuned expression of programmed death 1 ligands in mature dendritic cells stimulated by CD40 ligand is critical for the induction of an efficient tumor specific immune response. Cell Mol Immunol. 2008;5:33-9. (FC)<\/li>\n
  8. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Fr\u00f8ki\u00e6r H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC)<\/li>\n
  9. Jacobsen JT, Lunde E, Sundvold-Gjerstad V, Munthe LA, Bogen B. The cellular mechanism by which complementary Id+ and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunol Cell Biol. 2010;88:515-22. (FC)<\/li>\n
  10. Arora M, Poe SL, Oriss TB, Krishnamoorthy N, Yarlagadda M, Wenzel SE, et al. TLR4\/MyD88-induced CD11b+Gr-1intF4\/80+ non-migratory myeloid cells suppress Th2 effector function in the lung. Mucosal Immunol. 2010;3:578-93. (FC)<\/li>\n
  11. Duewell P, Kisser U, Heckelsmiller K, Hoves S, Stoitzner P, Koernig S, et al. ISCOMATRIX adjuvant combines immune activation with antigen delivery to dendritic cells in vivo leading to effective cross-priming of CD8+ T cells. J Immunol. 2011;187:55-63. (FC)<\/li>\n
  12. Szyszko EA, Skarstein K, Jonsson R, Brokstad KA. Distinct phenotypes of plasma cells in spleen and bone marrow of autoimmune NOD.B10.H2b mice. Autoimmunity. 2011;44:415-26. (FC)<\/li>\n
  13. Khare A, Krishnamoorthy N, Oriss TB, Fei M, Ray P, Ray A. Cutting edge: inhaled antigen upregulates retinaldehyde dehydrogenase in lung CD103+ but not plasmacytoid dendritic cells to induce Foxp3 de novo in CD4+ T cells and promote airway tolerance. J Immunol. 2013;191:25-29. (FC)<\/li>\n<\/ol>\n

    Spectral Red\u00ae is a registered trademark of Southern Biotechnology Associates, Inc.<\/p>\n

    Spectral Red\u00ae is a PE\/CY5 tandem conjugate<\/p>\n

    Cy\u00ae is a registered trademark of GE Healthcare<\/p>\n

    \u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u6297\u9ad4<\/strong><\/p>\n

    \u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
    1895-01<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-UNLB<\/strong><\/td>\nNIMR-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    1895-02<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-FITC<\/strong><\/td>\nNIMR-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    1895-08<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-BIOT<\/strong><\/td>\nNIMR-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    1895-09<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-PE<\/strong><\/td>\nNIMR-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    1895-09L<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-PE<\/strong><\/td>\nNIMR-4<\/td>\n0.2 mg<\/td>\n<\/tr>\n
    1895-11<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-APC<\/strong><\/td>\nNIMR-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    1895-13<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-SPRD<\/strong><\/td>\nNIMR-4<\/td>\n0.1 mg<\/td>\n<\/tr>\n
    1895-14<\/strong><\/a><\/td>\nRat Anti-Mouse MHC Class II-LE\/AF<\/strong><\/td>\nNIMR-4<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Isotype control <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a> | <\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a> | <\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA <\/strong>\u5957\u7d44<\/strong><\/a> | <\/strong>anti-rabbit <\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u6297\u5c0f\u9f20MHC II-SPRD \u00a0| \u8ca8\u865f1895-13\u00a0 \u00a0\u00a0 Rat Anti-Mouse MHC Clas […]<\/p>\n","protected":false},"author":3,"featured_media":4542,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[2583],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17755"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17755"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17755\/revisions"}],"predecessor-version":[{"id":20373,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17755\/revisions\/20373"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4542"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17755"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17755"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17755"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}