{"id":17668,"date":"2018-08-28T15:36:17","date_gmt":"2018-08-28T07:36:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17668"},"modified":"2022-07-20T16:04:36","modified_gmt":"2022-07-20T08:04:36","slug":"aldoc-adenovirus-human-abmgood%e8%b2%a8%e8%99%9f068583a","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17668","title":{"rendered":"ALDOC Adenovirus (Human)  | ABMGOOD\u8ca8\u865f068583A"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17668\"><strong>ALDOC Adenovirus (Human)<\/strong><strong> \u00a0| <\/strong><strong>\u8ca8\u865f068583A<\/strong><\/a><\/span><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u817a\u75c5\u6bd2\u5177\u6709\u975e\u5e38\u5ee3\u6cdb\u7684\u5bbf\u4e3b\u7bc4\u570d; \u5b83\u53ef\u4ee5\u611f\u67d3\u4eba\u548c\u5176\u4ed6\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7cfb\u6216\u539f\u4ee3\u7d30\u80de\uff0c\u5305\u62ec\u5206\u88c2\u548c\u975e\u5206\u88c2\u7d30\u80de\u3002 \u5be6\u969b\u4e0a\u5f88\u5c11\u6709\u7d30\u80de\u4fc2\u4e0d\u80fd\u88ab\u611f\u67d3\u3002 \u5df2\u7d93\u986f\u793a\u9020\u8840\u7d30\u80de\u4f86\u6e90\u7684\u7d30\u80de\u5c0d\u817a\u75c5\u6bd2\u611f\u67d3\u66f4\u5177\u6297\u6027\uff0c\u56e0\u6b64\u53ef\u80fd\u9700\u8981\u5927\u91cf\u75c5\u6bd2\u4ee5\u9054\u5230\u8db3\u5920\u7684\u611f\u67d3\u6c34\u5e73\uff0c\u56e0\u70ba\u90a3\u4e9b\u7d30\u80de\u50c5\u5728\u7d30\u80de\u819c\u4e0a\u8868\u9054\u6709\u9650\u7684CAR\u53d7\u9ad4\u3002\u817a\u75c5\u6bd2\u662fabmgood\u817a\u75c5\u6bd2\u8868\u9054\u7cfb\u7d71\u7522\u54c1\u4e4b\u4e00\uff0c\u53ef\u4ee5\u76f4\u63a5\u7528\u65bc\u5728\u591a\u7a2e\u5bbf\u4e3b\u7d30\u80de\u4e2d\u77ac\u6642\u904e\u8868\u9054\u611f\u8208\u8da3\u7684\u57fa\u56e0\u3002 \u8a72\u817a\u75c5\u6bd2\u53ef\u901a\u904e\u8f49\u5c0eHEK293\u7d30\u80de\u7528\u65bc\u64f4\u589e\u66f4\u591a\u7684\u817a\u75c5\u6bd2\u3002 \u70ba\u4e86\u63d0\u9ad8\u8f49\u5c0e\u6548\u7387\uff0c\u5efa\u8b70\u5728\u8f49\u5c0e\u6642\u4f7f\u75281\uff1a100\u7684ViralPlus\uff08G698\uff09\u3002\u9776\u7d30\u80de\u4e2d\u817a\u75c5\u6bd2\u611f\u67d3\u7684\u6548\u7387\u6aa2\u67e5\uff0c\u61c9\u7528\u4e00\u4e9b-Gal\u6216GFP\u6a19\u8a18\u817a\u75c5\u6bd2\uff0c\u5982Ad-CMV-\u03b2-Gal\u6216Ad-GFP\uff0c\u8a55\u4f30\u9776\u7d30\u80de\u7684\u611f\u67d3\u6548\u7387\u3002<span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"https:\/\/www.abmgood.com\/CMV%20Null-Adenovirus-000047A.html\">\u7a7aVector\u75c5\u6bd2\u6db2 (Adeno CMV-null) Cat. No. 000047A<\/a><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/premadeAdeno\/images\/Adenovirus-Procedure.jpg\" alt=\"\" width=\"801\" height=\"258\" \/><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>Vector<\/strong><\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/ALDOC-Adenovirus-068583A.html\">pAdenoG<\/a><\/td>\n<\/tr>\n<tr>\n<td><strong>Vector Size<\/strong><\/td>\n<td>32908bp<\/td>\n<\/tr>\n<tr>\n<td><strong>Promoter<\/strong><\/td>\n<td>CMV<\/td>\n<\/tr>\n<tr>\n<td><strong>Insert<\/strong><\/td>\n<td>ALDOC<\/td>\n<\/tr>\n<tr>\n<td><strong>Insert Size<\/strong><\/td>\n<td>1095 bp<\/td>\n<\/tr>\n<tr>\n<td><strong>Organism<\/strong><\/td>\n<td>Human<\/td>\n<\/tr>\n<tr>\n<td><strong>Accession #<\/strong><\/td>\n<td><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/BC065565\">BC065565<\/a><\/td>\n<\/tr>\n<tr>\n<td><strong>Chromosome<\/strong><\/td>\n<td>17<\/td>\n<\/tr>\n<tr>\n<td><strong>Tags<\/strong><\/td>\n<td>No Tags<\/td>\n<\/tr>\n<tr>\n<td><strong>Gene Type<\/strong><\/td>\n<td>Mutants can be constructed via custom services. Please refer to our\u00a0<a href=\"https:\/\/www.abmgood.com\/Custom-Recombinant-Adenovirus-Service.html\">custom service page<\/a>\u00a0or contact our\u00a0<a href=\"mailto:technical@abmGood.com\">technical support\u00a0<\/a>for more info.<\/td>\n<\/tr>\n<tr>\n<td><strong>Titer<\/strong><\/td>\n<td>&gt;1&#215;10<sup>6<\/sup>pfu\/mL<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Buffer<\/strong><\/td>\n<td>DMEM with 2.5% glycerol<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u7522\u54c1\u8a3b\u89e3<\/strong><strong>:<\/strong> \u75c5\u6bd2\u5c07\u7528\u65bc\u9ad4\u5916\u7d30\u80de\u57f9\u990a\uff0c\u4e0d\u9700\u8981\u96d9CsCl\u7d14\u5316\u3002 \u9ad4\u5167\u7814\u7a76\uff08\u5373\u52d5\u7269\u7814\u7a76\uff09\uff0c\u7d14\u5316\u5c0d\u65bc\u53bb\u9664\u6709\u7f3a\u9677\u7684\u9846\u7c92\uff0c\u7d30\u80de\u788e\u7247\u548c\u5c11\u91cf\u57f9\u990a\u57fa\u7d44\u5206\u662f\u5fc5\u9700\u7684\uff0c\u907f\u514d\u6c61\u67d3\u7269\u8a98\u5c0e\u986f\u8457\u7684\u514d\u75ab\u53cd\u61c9\u3002CsCl\u7d14\u5316\u5c07\u75c5\u6bd2\u6fc3\u7e2e\u81f3\u9069\u5408\u9ad4\u5167\u6ce8\u5c04\u7684\u91cf\u3002\u75c5\u6bd2\u9846\u7c92\uff08VPs\uff09\u4ee3\u8868\u75c5\u6bd2\u9846\u7c92\u7684\u7e3d\u6578\uff08\u611f\u67d3\u548c\u611f\u67d3\u7f3a\u4e4f\u7684\u7d44\u5408\uff09\u3002 \u7531\u65bc\u75c5\u6bd2\u88fd\u5291\u7684\u8b8a\u5316\uff0c\u611f\u67d3\u6027\/\u975e\u611f\u67d3\u6027\u7684\u6bd4\u4f8b\u986f\u8457\u8b8a\u5316\uff0c\u56e0\u6b64\uff0cVP\u4e0d\u53cd\u6620\u88fd\u5291\u4e2d\u75c5\u6bd2\u7684\u6fc3\u5ea6\u3002 PFU\uff08\u566c\u6591\u5f62\u6210\u55ae\u4f4d\uff09\u4ee3\u8868\u611f\u67d3\u6027\u6216\u6d3b\u75c5\u6bd2\u7684\u6578\u91cf\u3002 \u5b83\u53cd\u6620\u4e86\u88fd\u5291\u4e2d\u611f\u67d3\u6027\u75c5\u6bd2\u7684\u6fc3\u5ea6\u3002 IFU\uff08\u611f\u67d3\u55ae\u4f4d\uff09\u5728\u751f\u7269\u5b78\u4e0a\u8207PFU\u76f8\u7576\u3002 \u5c0d\u65bc\u5927\u591a\u6578\u75c5\u6bd2\u88fd\u5291\uff0cVP \/ PFU\u6bd4\u4f8b\u70ba20\uff1a1\u81f350\uff1a1\u3002<\/p>\n<table width=\"90%\">\n<tbody>\n<tr>\n<td width=\"32%\"><strong>Service<\/strong><\/td>\n<td width=\"55%\"><strong>Description<\/strong><\/td>\n<td width=\"12%\"><strong>Cat. No.<\/strong><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"4\"><strong>Adenovirus Purification<\/strong><\/td>\n<td><strong>Amplify and purify (2-3 ml of 10<sup>9<\/sup>\u00a0pfu\/ml)<\/strong><\/td>\n<td><strong>C070<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Amplify and purify (2-3 ml of 10<sup>10<\/sup>\u00a0pfu\/ml)<\/strong><\/td>\n<td><strong>C071<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Amplify and purify (5 x 200 \u03bcl of 10<sup>11<\/sup>\u00a0pfu\/ml)<\/strong><\/td>\n<td><strong>C072<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Amplify and purify (5 x 200 \u03bcl of 10<sup>12<\/sup>\u00a0pfu\/ml)<\/strong><\/td>\n<td><strong>C005<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ALDOC Adenovirus (Human) \u00a0| \u8ca8\u865f068583A \u7522\u54c1\u4ecb\u7d39: \u817a\u75c5\u6bd2\u5177\u6709\u975e\u5e38\u5ee3\u6cdb\u7684\u5bbf [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3420,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2595],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17668"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17668"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17668\/revisions"}],"predecessor-version":[{"id":17669,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17668\/revisions\/17669"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3420"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17668"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17668"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17668"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}