{"id":17480,"date":"2018-08-16T11:55:59","date_gmt":"2018-08-16T03:55:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17480"},"modified":"2023-03-13T12:34:26","modified_gmt":"2023-03-13T04:34:26","slug":"%e8%9b%8b%e7%99%bd%e8%b3%aa%e7%be%b0%e5%9f%ba%e5%90%ab%e9%87%8f%e6%aa%a2%e6%b8%ac-protein-carbonyl-content-assay-kit-fluorometric%e8%b2%a8%e8%99%9fk563-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17480","title":{"rendered":"\u86cb\u767d\u8cea\u7fb0\u57fa\u542b\u91cf\u6aa2\u6e2c | Protein Carbonyl Content Assay Kit (Fluorometric)\u8ca8\u865fK563-100 (ABCAM,ab235631)"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=17480\">\u86cb\u767d\u8cea\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K563-100<\/a>\u00a0\u00a0(ABCAM,ab235631)<\/span><\/p>\n<p><strong><em>Protein Carbonyl Content Assay Kit (Fluorometric)<\/em><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/5\/k563.jpg\" alt=\"\" \/><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u9577\u671f\u5347\u9ad8\u7684\u8840\u7cd6\u6c34\u5e73\u6703\u7522\u751f\u7cd6\u7684\u5fae\u74b0\u5883\uff0c\u8207\u975e\u9176\u4fc3\u52a0\u5408\u7269\u7684\u6c28\u57fa\u9178\u5171\u50f9\u53cd\u61c9\uff0c\u767c\u751fProtein carbonylation\u86cb\u767d\u8cea\u7fb0\u57fa\u5316\u3002Oxidative stress\u6c27\u5316\u61c9\u6fc0ROS\u7684\u904e\u91cf\u7522\u751f\uff0c\u4e5f\u53ef\u4ee5\u7522\u751fProtein carbonylation\u86cb\u767d\u8cea\u7fb0\u57fa\u5316\u5408\u7269\u3002\u96a8\u8457\u6642\u9593\u7684\u63a8\u79fb\uff0c\u9019\u5169\u500b\u904e\u7a0b\u6703\u7522\u751f\u86cb\u767d\u8cea\u7684\u9ad8\u7d1a\u7cd6\u57fa\u5316\u7d42\u7522\u7269\uff08AGEs\uff09\u3002\u86cb\u767d\u8cea\u53cd\u61c9\u6027AGEs\u7684\u4e3b\u8981\u529f\u80fd\u7d50\u679c\u662f\u9176\u6d3b\u6027\u964d\u4f4e\u3002\u53ef\u6eb6\u6027AGE\u9084\u53ef\u4ee5\u7d50\u5408\u4f75\u6fc0\u6d3b\u665a\u671f\u7cd6\u57fa\u5316\u7d42\u7522\u7269\uff08advanced glycation end-products , RAGE\uff09\u7684\u53d7\u9ad4\u3002Receptor-dependent outcomes\u53d7\u9ad4\u4f9d\u8cf4\u6027\u7d50\u679c\u5c0e\u81f4tissue dysfunction,\u7d44\u7e54\u529f\u80fd\u969c\u7919\u3001oxidative stress\u6c27\u5316\u61c9\u6fc0\u548cactivation of monocytes\u55ae\u6838\u7d30\u80de\u6d3b\u5316\u3001endothelial cells\u5167\u76ae\u7d30\u80de\u548cmesangial cells\u7cfb\u819c\u7d30\u80de\u7684\u6d3b\u5316\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/5\/k563-b.jpg\" alt=\"\" width=\"353\" height=\"356\" \/><\/p>\n<p>\u5728Alzheimer\u2019s Disease\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5\uff08AD\uff09\u4e2d\uff0c\u7fb0\u57fa\u5316\u5408\u7269\u662f\u5927\u8166\u4e2d\u6c27\u5316\u61c9\u6fc0\u7684\u6a19\u8a8c\u7269\uff0c\u4e26\u4e14\u5728\u8840\u6e05\u548c\u8840\u6f3f\u4e2d\u8207\u8a8d\u77e5\u56b4\u91cd\u7a0b\u5ea6\u6210\u6bd4\u4f8b\u5730\u5347\u9ad8\u3002 BioVision\u7684\u86cb\u767d\u8cea\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\u4f7f\u7528Fluoroscein-5-Thiosemicarbazide (FTC)\u87a2\u5149\u63a2\u91dd\uff08FTC\uff09\uff0c\u87a2\u5149\u63a2\u91dd\u8207\u86cb\u767d\u8cea\u4e0a\u7684\u6c27\u5316\u6b98\u57fa\uff08\u5373\u534a\u80f1\u6c28\u9178\uff0c\u8cf4\u6c28\u9178\uff0c\u7cbe\u6c28\u9178\uff0c\u7d44\u6c28\u9178\u548c\u5929\u51ac\u6c28\u9178\uff09\u5171\u50f9\u53cd\u61c9\u3002\u901a\u904e\u7522\u751f\u7a69\u5b9a\u7684\u87a2\u5149\u4fe1\u865f\uff08Ex \/ Em 485 \/535nm\uff09\u78ba\u5b9a\u86cb\u767d\u8cea\u7fb0\u57fa\u542b\u91cf\uff0c\u4e26\u8207\u5728BCA\u6e2c\u5b9a\u4e2d\u6e2c\u5b9a\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u9032\u884c\u6bd4\u8f03\uff0c\u4ee5\u5b9a\u91cf\u7fb0\u57fa\/ mg\u86cb\u767d\u8cea\u7684nmoles\u3002\u8a72\u8a66\u5291\u76d2\u64cd\u4f5c\u7c21\u55ae\uff0c\u4e0d\u9700\u8981\u523a\u6fc0\u6027\u5316\u5b78\u7269\u8cea\uff0c\u53ef\u4ee5\u5b9a\u91cf\u8840\u6e05\u6216\u8840\u6f3f\u4e2d\u7684\u7fb0\u57fa\u5316\u5408\u7269\uff0c\u4e26\u4e14\u6bd4\u540c\u7b49\u6bd4\u8272\u6e2c\u5b9a\u6cd5\u7522\u751f\u66f4\u53ef\u9760\u548c\u53ef\u91cd\u8907\u7684\u7d50\u679c\u3002\u5b83\u53ef\u4ee5\u6aa2\u6e2c\u86cb\u767d\u8cea\u6fc3\u5ea6\u4f4e\u81f31\u6beb\u514b\/\u6beb\u5347\u7684\u6a23\u54c1\u4e2d\u7684\u7fb0\u57fa\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/5\/k563-c.jpg\" alt=\"\" width=\"390\" height=\"285\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K563-100\u00a0\u00a0(ABCAM,ab235631)<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Summary<\/strong><\/td>\n<td>\u2022 Detection method- Fluorometric (Ex\/Em 485\/535 nm)<br \/>\n\u2022 Species reactivity- Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorometric (Ex\/Em 485\/535 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Quantification of carbonyl content as a putative biomarker for research in disease areas such as diabetes, multiple sclerosis, and Alzheimer\u2019s diseas<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>Simple procedure; validated with human serum samples containing pmol of carbonylated protein<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Protein Carbonyl Assay Buffer<br \/>\n\u2022 100% TCA Solution<br \/>\n\u2022 10% Streptozocin Solution<br \/>\n\u2022 6 M Guanidine Solution<br \/>\n\u2022 Sample Dilution Buffer<br \/>\n\u2022 FTC (10 mM in DMSO)<br \/>\n\u2022 Positive Control Protein (10 mg\/ml)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u86cb\u767d\u8cea\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK563-100\u00a0\u00a0(ABCAM,ab235631) Protein Car [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2619],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17480"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17480"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17480\/revisions"}],"predecessor-version":[{"id":40005,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17480\/revisions\/40005"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17480"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17480"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17480"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}