{"id":17453,"date":"2018-08-13T17:59:58","date_gmt":"2018-08-13T09:59:58","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17453"},"modified":"2022-07-20T16:11:45","modified_gmt":"2022-07-20T08:11:45","slug":"%e5%89%8d%e8%84%82%e8%82%aa%e7%b4%b0%e8%83%9e%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk583-5","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17453","title":{"rendered":"\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2 | Lipolysis (Adipocyte) Colorimetric\/Fluorometric Assay Kit \u8ca8\u865fK581-5"},"content":{"rendered":"

\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K581-5<\/a><\/span><\/p>\n

Lipolysis (Adipocyte) Colorimetric\/Fluorometric Assay Kit <\/em><\/strong>Only kit on the market to isolate adipocytes from adipose tissue & measure lipolysis<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8102\u80aa\u5206\u89e3\u662f\u7518\u6cb9\u4e09\u916f\u7d30\u80de\u5167\u6c34\u89e3\u6210\u7518\u6cb9\u548c\u6e38\u96e2\u8102\u80aa\u9178\uff0c\u7136\u5f8c\u91cb\u653e\u5230\u8840\u6d41\u6216\u57f9\u990a\u57fa\u4e2d\u3002\u8102\u89e3\u4f5c\u7528\u57fa\u672c\u4e0a\u5728\u6240\u6709\u7d30\u80de\u4e2d\u767c\u751f\uff0c\u4f46\u5728\u767d\u8272\u548c\u68d5\u8272\u8102\u80aa\u7d44\u7e54\u4e2d\u6700\u8c50\u5bcc\u3002\u8102\u80aa\u5206\u89e3\u7684\u7f3a\u9677\u5c0e\u81f4\u7d30\u80de\u5167\u8102\u8cea\u7a4d\u7d2f\u589e\u52a0\uff0c\u5c0e\u81f4\u7570\u5e38\u7684\u7d30\u80de\u751f\u7406\u5b78\uff0c\u9ad8\u8102\u8840\u75c7\u548c\u80f0\u5cf6\u7d20\u62b5\u6297\u3002\u6fc0\u7d20\u6216\u5152\u8336\u915a\u80fa\u53ef\u8a98\u5c0e\u8102\u80aa\u5206\u89e3\uff0c\u5176\u8207\u03b2-\u814e\u4e0a\u817a\u7d20\u80fd\u53d7\u9ad4\u7d50\u5408\uff0c\u5c0e\u81f4\u817a\u82f7\u9178\u74b0\u5316\u9176\u6d3b\u5316\uff0c\u5f9eATP\u5f62\u6210cAMP\u3002 cAMP\u4f5c\u70ba\u6fc0\u6d3b\u6fc0\u7d20\u654f\u611f\u6027\u8102\u80aa\u9176\u7684\u7b2c\u4e8c\u4fe1\u4f7f\uff0c\u5176\u6c34\u89e3\u7518\u6cb9\u4e09\u916f\u3002\u8a72\u9014\u5f91\u53d7\u80f0\u5cf6\u7d20\u6291\u5236\u3002 BioVision\u7684\u8102\u80aa\u7d30\u80de\u8102\u80aa\u5206\u89e3\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u76e3\u6e2c\u8102\u80aa\u5206\u89e3\u7684\u7c21\u4fbf\u65b9\u6cd5\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u5f9e\u591a\u90545g\u7684\u5c0f\u9f20\u6216\u5927\u9f20\u8102\u80aa\u7d44\u7e54\u4e2d\u5206\u96e2\u8102\u80aa\u7d30\u80de\u6240\u9700\u7684\u6240\u6709\u8a66\u5291\uff0c\u4e26\u5728\u8a98\u5c0e\u8102\u89e3\u5f8c\u6e2c\u91cf\u4f86\u81ea\u539f\u4ee3\u8102\u80aa\u7d30\u80de\u7684\u7518\u6cb9\u91cb\u653e\u3002\u5b83\u9084\u5305\u62ec\u5408\u6210\u7684\u5152\u8336\u915a\u80fa\uff0c\u7570\u4e19\u814e\u4e0a\u817a\u7d20\uff0c\u4ee5\u523a\u6fc0cAMP\u4ecb\u5c0e\u7684\u9014\u5f91\u3002\u4fe1\u865f\u5f37\u5ea6\u8207\u5b58\u5728\u7684\u7518\u6cb9\u91cf\u6210\u6b63\u6bd4\u3002\u8a72\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u4ee5\u5728\u6bd4\u8272\u6e2c\u5b9a\u4e2d\u6aa2\u6e2c\u5c0f\u65bc200pmol\u7684\u7518\u6cb9\uff0c\u87a2\u5149\u6e2c\u5b9a\u4e2d\u6aa2\u6e2c\u5c0f\u65bc20pmol\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK581-5<\/td>\n<\/tr>\n
Size<\/strong><\/td>\nFor 5 g Tissue<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 570 nm) and Fluorescence (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIsolation of adipocytes from rat\/mouse tissue
\nMeasurement of lipolysis by adipocytes
\nScreening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient method to isolate adipocytes from adipose tissue & measure lipolysis.
\n\u2022 The assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Collagenase (0.2%)
\n\u2022 Collagenase Stop Buffer
\n\u2022 Adipocyte Wash Buffer
\n\u2022 Adipocyte Lipolysis Buffer
\n\u2022 Glycerol Assay Buffer
\n\u2022 Glycerol Probe (in DMSO, anhydrous)
\n\u2022 Glycerol Enzyme Mix (Lyophilized)
\n\u2022 Glycerol Standard (100 mM)
\n\u2022 Isoproterenol (10 mM)
\n\u2022 Cell strainer (100 \u00b5m)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865fK581-5 Lipolysis (Adipocyte) Colorimetri […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[466,124],"tags":[2623],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17453"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17453"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17453\/revisions"}],"predecessor-version":[{"id":25671,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17453\/revisions\/25671"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17453"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17453"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}