{"id":17450,"date":"2018-08-13T17:53:04","date_gmt":"2018-08-13T09:53:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17450"},"modified":"2023-04-11T14:36:47","modified_gmt":"2023-04-11T06:36:47","slug":"%e5%89%8d%e8%84%82%e8%82%aa%e7%b4%b0%e8%83%9e%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-preadipocyte-isolation-kit%e8%b2%a8%e8%99%9fk583-5","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17450","title":{"rendered":"\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | Preadipocyte Isolation Kit\u8ca8\u865fK583-5 (Abcam ab196988)"},"content":{"rendered":"

\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K583-5<\/a>\u00a0(Abcam ab196988)\u00a0<\/span><\/p>\n

Preadipocyte Isolation Kit (\u5305\u62ecCell Strainer\u3001Collagenase\u3001Red Blood Cell Lysis Buffer\u3001PBS)<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u8102\u80aa\u7d44\u7e54\u662f\u758f\u9b06\u7684\u7d50\u7de0\u7d44\u7e54\uff0c\u5728\u52d5\u7269\u9ad4\u5167\u7a4d\u805a\uff0c\u4e3b\u8981\u7528\u65bc\u5132\u5b58\u80fd\u91cf\uff0c\u4fdd\u6eab\u548c\u9ad4\u6eab\u8abf\u7bc0\u3002\u5b83\u4e3b\u8981\u7531\u8102\u80aa\u7d30\u80de\u7d44\u6210\uff0c\u4f46\u4e5f\u542b\u6709\u7531\u524d\u8102\u80aa\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\uff0c\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u548c\u514d\u75ab\u7d30\u80de\u7d44\u6210\u7684\u57fa\u8cea\u8840\u7ba1\u90e8\u5206\u3002\u6700\u8fd1\u7684\u7814\u7a76\u767c\u73fe\u4e86\u8102\u80aa\u7d44\u7e54\u5728\u5404\u7a2e\u6fc0\u7d20\u7684\u7522\u751f\u548c\u5206\u6ccc\u4e2d\u4ee5\u53ca\u4f5c\u70ba\u6d3b\u8e8d\u7684\u5167\u5206\u6ccc\u5668\u5b98\u7684\u52d5\u614b\u4f5c\u7528\u3002\u8102\u80aa\u7684\u904e\u5ea6\u7a4d\u7d2f\u88ab\u7a31\u70ba\u80a5\u80d6\u75c7\u3002\u9632\u6b62\u8102\u80aa\u904e\u5ea6\u7a4d\u7d2f\u7684\u65b9\u6cd5\u662f\u4e00\u500b\u6df1\u5165\u7814\u7a76\u7684\u9818\u57df\u3002\u9664\u80a5\u80d6\u5916\uff0c\u5df2\u767c\u73fe\u8102\u80aa\u7d44\u7e54\u53c3\u8207\u5404\u7a2e\u751f\u7406\u904e\u7a0b\uff0c\u5305\u62ec\u751f\u6b96\uff0c\u8840\u7ba1\u751f\u6210\uff0c\u708e\u75c7\uff0c\u764c\u75c7\u548c\u8840\u7ba1\u5167\u7a69\u614b\u3002<\/p>\n

BioVision\u7684\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2<\/a>(Preadipocyte Isolation Kit, K583) \u63d0\u4f9b\u4e86\u5f9e\u8102\u80aa\u7d44\u7e54(adipose tissue)\u4e2d\u5206\u96e2\u57fa\u8cea\u8840\u7ba1\u7d44\u5206(\u00a0stromal vascular fraction)\u7684\u8a66\u5291\u548c\u5de5\u5177\u3002\u5c07\u5f97\u5230\u7684\u57fa\u8cea\u8840\u7ba1\u90e8\u5206\u5728\u7d44\u7e54\u57f9\u990a\u677f(tissue culture plate)\u4e0a\u57f9\u990a\uff0c\u5176\u4e2d\u524d\u8102\u80aa\u7d30\u80de\u7c98\u9644(\u00a0preadipocytes adhere)\u3002\u7576\u7528\u8a98\u5c0e\u5206\u5316\u7684\u7d44\u5206\uff08Cat\u3002\uff03K579,3T3-L1\u5206\u5316\u8a66\u5291\u76d2\uff09\u8655\u7406\u6642\uff0c\u524d\u8102\u80aa\u7d30\u80de\u4fdd\u7559\u589e\u6b96(proliferate )\u548c\u5206\u5316\u6210\u8102\u80aa\u7d30\u80de(\u00a0differentiate into adipocytes)\u7684\u80fd\u529b\u3002\u524d\u8102\u80aa\u7d30\u80de(preadipocytes)\u53ef\u7528\u65bc\u7814\u7a76\u8102\u80aa(adipogenesis)\u751f\u6210\u904e\u7a0b\uff0c\u5206\u5316\u7684\u8102\u80aa\u7d30\u80de(adipocytes)\u53ef\u7528\u65bc\u7814\u7a76\u8102\u80aa\u5206\u89e3(lipolysis)\uff0c\u5167\u5206\u6ccc\u6d3b\u52d5(endocrine activity)\uff0c\u7d30\u80de\u4fe1\u865f\u50b3\u5c0e(cell-signaling )\u548c\u4ee3\u8b1d\u529f\u80fd\u969c\u7919(metabolic dysfunction)\u3002<\/p>\n

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\u6cb9\u7d05 O \u8102\u80aa\u67d3\u8272\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<\/span>\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u00a0<\/a>|<\/span>\u00a0\u8102\u80aa\u7d30\u80de\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a>\u00a0|<\/span>\u00a0\u55ae\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/span><\/strong><\/span><\/p>\n

\"\"<\/p>\n

\u793a\u4f8b\u6578\u64da\u00a0<\/strong><\/span>\u4f7f\u7528 3T3-L1 \u5206\u5316\u8a66\u5291\u76d2\u5f9e\u524d\u8102\u80aa\u7d30\u80de\u5206\u5316 7 \u5929\u540e\u7684\u8102\u80aa\u7d30\u80de\u3002\u53ef\u898b\u7684\u8102\u6ef4\u8b49\u5be6\u4e86\u524d\u8102\u80aa\u7d30\u80de\u7684\u5206\u96e2\u3002<\/div>\n

\u5f15\u7528\u6587\u737b<\/span> <\/strong>Singh SP \u00a0et al.<\/em>\u00a0Kavain Reduces Porphyromonas gingivalis-Induced Adipocyte Inflammation: Role of PGC-1a Signaling.\u00a0J Immunol<\/em>\u00a0N\/A:N\/A (2018).<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK583-5 (Abcam ab196988)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\nFor 5 g Tissue<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIsolation of preadipocytes from adipose tissue<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient method to isolate preadipocytes from adipose tissue.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Collagenase (0.2%)
\n\u2022 Collagenase Stop Buffer
\n\u2022 Adipocyte Wash Buffer
\n\u2022 Adipocyte Lipolysis Buffer
\n\u2022 Glycerol Assay Buffer
\n\u2022 Glycerol Probe (in DMSO, anhydrous)
\n\u2022 Glycerol Enzyme Mix (Lyophilized)
\n\u2022 Glycerol Standard (100 mM)
\n\u2022 Isoproterenol (10 mM)
\n\u2022 Cell strainer (100 \u00b5m)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong>\u00a0\u00a0\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7518\u6cb9\u4e09\u916f\uff08TG\uff09\u87a2\u5149\u6aa2\u6e2c<\/a>-384well |\u00a0\u4e09\u9178\u7518\u6cb9\u916f\u5448\u8272\/\u87a2\u5149\u5206\u6790<\/a>\u00a0|\u00a0\u809d\u8102\u80aa\u8b8a\u6027\u5206\u6790\u8a66\u5291\u76d2<\/strong><\/a><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u524d\u8102\u80aa\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865fK583-5\u00a0(Abcam ab196988)\u00a0 Preadipocyte Is […]<\/p>\n","protected":false},"author":3,"featured_media":17451,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2624],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17450"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17450"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17450\/revisions"}],"predecessor-version":[{"id":40964,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17450\/revisions\/40964"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17451"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17450"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}